HSV,CMV,EBV,HHV6,HHV8,Adeno,Parvo,JC/BK,HPV,Pox Flashcards
-Cell Culture -HSV -CMV -EBV -HHV6 -HHV8 -Adenovirus -Porvovirus -JC/BK Virus -HPV
Viral culture Cytopathic Effect (CPE):
-Tear shaped cells
Enterovirus
Viral culture Cytopathic Effect (CPE):
-CMV
Focal plaques in HDF
Viral culture Cytopathic Effect (CPE):
-Grape-like clusters
Adenovirus
Viral culture Cytopathic Effect (CPE):
-RSV
Syncytial cells
Viral culture Cytopathic Effect (CPE):
-HSV
Sweeping, globular cells
A positive IgG antibody may indicate what?
Current or Past infection
A positive IgM antibody indicates what?
Current infection
Viral Histology - RSV:
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
- Notes
- NONE
- Yes
Viral Histology - HSV :
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
-Notes (Cowdry type “?”, 3Ms)
- Nuclear
- Yes
- Cowdry type A
- 3Ms (Multinucleated, Molded nuclei, Marginated chromatin)
Viral Histology - Adenovirus:
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
-Notes
- Nuclear
- No
-“Smudge Cells”
Viral Histology - CMV:
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
-Notes
- Nuclear&Cytoplasmic
- No
-“Owl eye” inclusion, NOT multinucleated
Viral Histology - Measles:
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
-Notes
- Nuclear&Cytoplasmic
- Yes
-“Warthin-Finkeldey Giant cells”
Viral Histology - Rabies:
- Nuclear/Cytoplasmic Inclusions
- Syncytia (Y/N)
- Notes
- Cytoplasmic
- No
-Negri bodies
Where does HSV type I go dormant?
Trigeminal Ganglia
Where does HSV type 2 go dormant?
Sacral Gangia
HSV definitive dx method.
Culture
-CPE within 2-3 days
Neonatal CMV features.
- Intracerebral calcifications
- Petechial Rash (coag)
- microcephaly
EBV pathogenesis:
Pharyngeal or genital mucosa
-Infects B-lymphs via C3d receptor (CD21)
What type of lymphocyte is seen in p. smear of EBV. (Atypical lymphocytosis)
-CD8+ T lymphocytes
What is the site of EBV latency?
B-lymphocytes
Uncontrolled activation of T/NK cells following EBV infection, leading to fuliminant primary EBC, that is frequently fatal
Duncan disease (X linked immunoproliferative disease) -SH2D1A (SAP) gene
What is the monospot test based upon?
Frequent presence of heterophile antibodies in EBV infection
IHC stain that performs nearly as well as EBER.
-LMP1
Virus responsible for a significant proprotion of child hood febrile seizures (highly neurotropic; may cause encephalitis)
-HHV6
HHV8 associations. (3)
- Kaposi sarcoma
- Primary Effusion Lymphoma
- Kastleman’s Dz in HIV+
Adenovirus serotypes in most respiratory infections.
1-14 & 21
Adenovirus serotypes ass. w/ hemorrhagic cystitis
11 & 21
Adenovirus serotypes ass. w/ childhood gastritis
40 & 41
Virus associated with Aplastic Anemia.
Parvovirus B19 (Erythrovirus)
Where do JC & BK viruses lay dormant?
Urothelium and Lymphocytes
Reactivation of JC virus features.
- PML - Infects Oligos
- AIDS (MC setting)
- Smudgy nuclear inclusions in oligos
Reactivation of BK virus features.
- Hemorrhagic cystitis and/or
- Polyomavirus nephropathy
- Transplant (MC setting)
- Decoy cells - smudgy nuclear inclusions
HPV type (order of frequency): -Common wart
2, 1, 4
HPV type (order of frequency): -Genital warts (condyloma acuminatum)
6, 11
HPV type (order of frequency): -LSIL
6, 11
HPV type (order of frequency): -HSIL
16, 18, 31, 33, 35
HPV type (order of frequency): -AIS/Cervical Adenoca
18, 16, 45
