Fastidious Gram Neg Bacilli Flashcards
1
Q
General characteristic of Fastidious Gram Negative Bacilli?
A
- NO growth on MacConkey
- Slow growth on enriched media
- Pinpoint colonies
2
Q
Fastidious Gram Negative Bacilli - Organisms
A
- Francisella tularensis
- Brucella
- Bordetella
- Steptobacillus maniliformis
- Legionella
- Capnocytophagia canimorsus
- Pasteurella
- Camplylobactor jejuni
- H. pylori
- Haemophilus spp.
- Coxiella
- HACEK group
3
Q
Francisella tularensis:
- Requires
- Oxidase
- Urease
- B-lactamase
- Causes
A
Francisella tularensis: -Requires Sulfhydryl compounds (cysteine/cystine) -Oxidase NEGATIVE -Urease NEGATIVE -B-lactamase POSITIVE Causes Tularemia: -Rabbit fever -Deer Fly fever
4
Q
Brucella:
- Culture medium/condition
- Transmission
- Clinical Sxs (6)
A
Brucella:
- Chocolate agar w/ CO2
- Lifestock/Unpasteurized Dairy
- Fever w/ Lymphadenopathy
- Hepatosplenomegaly
- Malodorous sweat
- Spontaneous abortion
- Hepatitis (Granulomas)
- Endocarditis (Ass w/ Mortality)
5
Q
Bordetella:
- Requires (3)
- Culture media (2)
- Culture condition
- Colony morphology
- Causes
A
- Nicotinic Acid
- Cysteine
- Methionine
- Bordet-Gengou/Regan-Lowe media
- 35C w/ CO2
- Whooping cough
6
Q
Streptobacillus moniliformis:
- Media
- Colony morphology
- Gram Stain
- Causes
A
- Thioglycolate Broth
- Puffball/Cottonball morphology
- Tangles of Gram Variable w/ filamentous rods w/ moniliform swellings
- Rat Bite Fever
7
Q
Legionella:
- Requires
- Agar
- Colony morphology
- Gram stain
- ID Confirmation
- MC Serogroup
A
- L-cysteine
- BCYE Agar (2-5 days)
- Whitesh colonies
- Gram INVISIBLE
- Direct fluorescent Ab/Latex Agglutination
- L. pneumophila (serogroup 1) - Urinary Ag
8
Q
Capnocytophaga canimorsus:
- Agar/Requirements
- Gram stain morph
- Catalase
- Oxidase
- Infection
A
- Chocolate agar w/ CO2
- Thin, spindle-shaped
- Catalase POSITIVE
- Oxidase POSITIVE
- Soft tissue infection (Dog/Cat bite)
9
Q
Pasteurella:
- Oxidase
- Catalase
- Indole
- Causes
A
Pasteurella:
- Oxidase POSITIVE
- Catalase POSITIVE
- Indole POSITIVE (Strong)
- ST infection (dog/cat bite)
10
Q
Campylobacter jejuni:
- Oxidase
- Catalase
- What distinguishes it from other Campy?
- Gram Stain
- Medium/Condition
A
Campylobacter jejuni:
- Oxidase POSITIVE
- Catalase POSITIVE
- Hippurate Hydrolysis POSITIVE
- “Seagull shaped” (tiny, curved)
- Campy BAP at 42C
11
Q
H. pylori:
- Gram stain
- Diagnosis Tests
- Antibody formation
A
- Spiral bacillus
- Urea breath test
- Stool antigen
- Histology
- IgG Anti-H.pylori at 4 weeks
12
Q
Haemophilus spp.
- Does not grow on what?
- Medium
- Colony morphology
A
- NO growth on MacConkey or SBA
- Chocolate agar
- Small colonies
13
Q
H. influenza:
- X-factor
- V-factor
- Hemolysis
A
H. influenza:
- X-factor - POSITIVE
- V-factor - POSITIVE
- Hemolysis - NEGATIVE
14
Q
H. parainfluenza:
- X-factor
- V-factor
- Hemolysis
A
H. parainfluenza:
- X-factor - NEGATIVE
- V-factor - POSITIVE
- Hemolysis - NEGATIVE
15
Q
H. haemolyticus:
- X-factor
- V-factor
- Hemolysis
A
H. haemolyticus:
- X-factor - POSITIVE
- V-factor - POSITIVE
- Hemolysis - POSITIVE
