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Biology > Monoclonal/ELISA > Flashcards

Monoclonal/ELISA Flashcards

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1
Q

What is a monoclonal antibody?

A

Antibodies with the same tertiary structure

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2
Q

How does a monoclonal antibody work?

A

Produced from cloned plasma cells
Only has one shape of variable region so is only complementary to one specific antigen

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3
Q

What is a polyclonal antibody?

A

a collection of many different types of
antibodies.
MANY shapes of variable region
Therefore they (collectively) are
complementary to MANY antigens

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4
Q

What does ELISA stand for?

A

Enzyme-linked Immunosorbent Assay

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5
Q

ELISA test model answer

A
  1. First antibody binds/complementary to the antigen of interest
  2. Second antibody with an enzyme is added
  3. Second antibody binds to first antibody/antigen
  4. Unbound antibody is washed away
  5. Solution added and colour change seen
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