Molecular Theory and Techniques

Question 1

True or False? Molecular arrays typically contain a single probe for high throughput analysis of gene expression or determination of mutations in patient samples.

Answer 1

False

Question 2

Which of the following is LEAST likely to affect the purification of genomic
DNA for analysis in molecular methods?
a. Sample volume required for hybridization
b. Size of the nucleic acid to be isolated
c. Presence of RNA in the sample
d. Presence of protein in the sample
e. Presence of deoxyribose

Answer 2

e. Presence of deoxyribose

Question 3

Why is it important to add RNase to the patient sample when isolating
genomic DNA?
a. RNase serves as an enzymatic tag on DNA probes.
b. RNase will remove excess collagen from the patient sample.
c. RNase will transport the amino acid to the ribosome, where the peptide
chain elongates.
d. RNase clones DNA to RNA.
e. RNase digests RNA in the patient sample that could copurify with
genomic DNA.

Answer 3

e. RNase digests RNA in the patient sample that could copurify with
genomic DNA.

Question 4

All of the following describe a nucleic acid probe except:
a. Short strand of DNA of a known sequence
b. Short strand of RNA of a known sequence
c. Can be cloned in bacteria
d. Can be inserted into a plasmid vector
e. Can be labeled with avidin

Answer 4

e. Can be labeled with avidin

Question 5

Of the following, which is a common example of solid support hybridization?
a. Southern blot
b. Chemiluminescence
c. Hybridization protection assay
d. Digene Hybrid Capture 2
e. In situ hybridization

Answer 5

a. Southern blot
e. In situ hybridization

Question 6

When sequencing DNA, stops DNA synthesis in polymerase chain reaction
amplification.
a. Dideoxynucleotides
b. DNA polymerase
c. Insertion of thymine
d. 3′-OH group
e. Pyrophosphates

Answer 6

a. Dideoxynucleotides

Question 7

One of the most common clinical applications for identifying SNPs in patients
is:
a. SNPs have been useful in optimizing the treatment of HIV.
b. SNPs have been useful in diagnosing metabolic syndrome.
c. SNPs have been used to facilitate transcription-mediated amplification.
d. Currently, there are no clinical applications for the identification of
SNPs as these are silent and have no apparent function.
e. SNPs are added to the patient sample during polymerase amplification
reactions to create the complimentary nucleic acid sequence.

Answer 7

a. SNPs have been useful in optimizing the treatment of HIV.

Question 8

The development of “real-time” PCR was driven by:
a. Learning that Taq polymerase that has 5′- to 3′ exonuclease activity
b. The development of a dot blot hybridization methods
c. The development of chain ligase reaction
d. The ability to volatilize a sample in a nebulizer
e. The development of scorpion primers

Answer 8

a. Learning that Taq polymerase that has 5′- to 3′ exonuclease activity