Immunochemical Techniques

Question 1

. The strength of binding between an antigen and antibody is related to the
a. Goodness of fit between the epitope and the F(ab)
b. Concentration of antigen and antibody
c. Source of antibody production, because monoclonal antibodies bind
better
d. Specificity of the antibody

Answer 1

a. Goodness of fit between the epitope and the F(ab)

Question 2

In monoclonal antibody production, the specificity of the antibody is
determined by the
a. Sensitized B lymphocytes
b. Myeloma cell line
c. Sensitized T lymphocytes
d. Selective growth medium

Answer 2

a. Sensitized B lymphocytes

Question 3

Which unlabeled immune precipitation method in gel is used to quantitate a
serum protein?
a. Radial immunodiffusion
b. Double diffusion
c. Counterimmunoelectrophoresis
d. Immunofixation electrophoresis

Answer 3

a. Radial immunodiffusion

Question 4

In immunofixation electrophoresis, discrete bands appear at the same
electrophoretic location, one reacted with antihuman IgA (α-chain–
specific) reagent, and the other reacted with antihuman λ reagent. This is
best described as
a. An IgA λ monoclonal protein
b. An IgA λ polyclonal protein
c. IgA biclonal proteins
d. Cross-reactivity

Answer 4

a. An IgA λ monoclonal protein

Question 5

In nephelometry, the antigen–antibody complex formation is enhanced in the
presence of
a. Polyethylene glycol
b. High ionic strength saline solution
c. Normal saline
d. Complement

Answer 5

a. Polyethylene glycol

Question 6

Which homogeneous immunoassay relies on inhibiting the activity of the
enzyme label when bound to antibody reagent to eliminate separating free
labeled from bound labeled reagent?
a. EMIT
b. CEDIA
c. MEIA
d. ELISA

Answer 6

a. EMIT

Question 7

In flow cytometry, the side scatter is related to the
a. Granularity of the cell
b. DNA content of the cell
c. Size of the cell
d. Number of cells in G0 and G1

Answer 7

a. Granularity of the cell

Question 8

You analyze the DNA content on a sample of breast tissue for suspected
malignancy using flow cytometry and get the following results: DI = 2.5
and % cells in S phase = 29%. Based on these results you can conclude
a. These results are likely indicative of a malignant breast tumor
b. This is normal breast tissue
c. These results are consistent with a mostly diploid population
d. The results are not consistent with one another; no information is
gained

Answer 8

a. These results are likely indicative of a malignant breast tumor

Question 9

The nucleic acid technique in which RNA is converted to cDNA, which is
then amplified, is known as
a. RT-PCR
b. PCR
c. RFLP
d. In situ hybridization

Answer 9

a. RT-PCR