Chromatography and Mass Spectrometry

Question 1

Which of the following statements is TRUE?
a. Partition chromatography is most appropriate for identifying analytes
that may be distributed between two liquid phases.
b. Steric exclusion chromatography is best suited for separating analytes
based on their solubility in the mobile solvent.
c. In liquid–solid chromatography, the stationary phase separates analytes
based on size, shape, and polarity.
d. Ion-exchange chromatography has a resin phase that is soluble to
water, and separation of the mixture is based on magnitude and charge
of ionic species.
e. The partition coefficient is measured and compared with standards in
thin-layer chromatography.

Answer 1

a. Partition chromatography is most appropriate for identifying analytes
that may be distributed between two liquid phases.

Question 2

In high-performance thin-layer chromatography (HPTLC), developed bands
are compared with reference standard concentrations. Each band is
measured by:
a. Mass spectrometer
b. Densitometer
c. Ruler
d. Biuret protein assay
e. Two-dimensional electrophoresis

Answer 2

b. Densitometer

Question 3

In which of the following components of a chromatography instrument does
selective separation of a mixture occur?
a. Sample injection port
b. Column
c. Spectrometer
d. Quadrupole
e. Mass analyzer

Answer 3

b. Column

Question 4

True or False? In chromatography, the stationary phase is always of a solid
matrix.

Answer 4

False

Question 5

Mass spectrometry identifies analytes based on:
a. Mass-to-charge ratio
b. Retention factor
c. Density of the band
d. Molecular weight
e. Solubility in the mobile phase

Answer 5

a. Mass-to-charge ratio

Question 6

Drugs of abuse are typically measured by:
a. Thin-layer chromatography
b. Liquid–liquid chromatography
c. Gas–liquid chromatography
d. Steric exclusion chromatography
e. HPLC

Answer 6

c. Gas–liquid chromatography

Question 7

PCR/ESI-TOF has the distinct advantage in pathogen identification because:
a. It requires the patient sample to be cultured and then analysis can be
performed
b. It can be used directly from patient specimens
c. It uses the protein “fingerprint” to identify the pathogen.

Answer 7

b. It can be used directly from patient specimens