Molecular Genetics: Part 1: The Search for Inheritable Material Flashcards
What is Bacterial Transformation?
The ability of bacteria to alter their genetic makeup by absorbing foreign DNA molecules from other bacterial cells and incorporating the foreign DNA into their own.
Who discovered Bacterial Transformation and when?
Griffith (1927)
What type of bacteria did Griffith work on?
Diplococcus Pneumoniae
What did Avery, McLeod, and McCarty(1944) do?
They provided evidence that supported Griffith discovery, and it was DNA.
What did Hershey and Chase(1952) do?
They proved that DNA, not protons, was the molecule of inheritance.
How did Hershey and Chase prove what is in the answer of this question?: What did Hershey and Chase(1952) do?
They tagged bacteriophage with radioactive isotopes: 35S and 32P. 32P labeled the DNA and 35S labeled the proton. Only 32P was found.
What did Rosalind Franklin (1950-1953) do?
She continued the work begun by Maurice Wilkin. She carried out the X-Ray Crystallography analysis of DNA that showed DNA to be a helix.
Note: Her work was critical to Watson and Crick.
How was Rosalind’s work critical to Watson and Crick?
Her work helped Watson and Crick develop the famous model of the DNA.
What did Meselson and Stahl(1953) do?
They proved Watson’s and Crick’s hypothesis that DNA replicates in a semiconservative fashion.
