Molecular Biology- DNA replication Flashcards
What are deoxynucleotide triphosphates (dNTPs) ?
A nucleotide containing 3 phosphate groups attached to the 5’ carbon of the deoxyribose sugar of the nucleoside
What are three types of dNTPs?
dATPs, dTTPs, dGTPs
Attachment of dNTPs requires the removal of ________?
2 phosphates (pyrophosphates)
T/F: DNA is synthesized 3’-5’.
F: 5’-3’
The origin of replication in DNA replication is at the construction of the ____________.
replisome
The origin of replication consists of ____ bp and has the highest concentration of ___ and ___.
250, A’s, T’s
Identification of replication is done by _____. How does this happen?
-DnaA proteins
-Up to 40 proteins will bind
-They mark the area and put pressure on H+ bonds so that they can break apart strands more easily
What is the second step of DNA replication?
Helicase (DnaB)
What does helicase (DnaB) do?
uses energy from ATP hydrolysis to pull strands apart
______ helps DnaB load onto strands.
DnaC
__________ relieve supercoiling tension.
Topoisomerases
T/F: DNA polymerase works slow making 10 bases/ sec.
F: fast- 1000 bases/ sec
What things does DNA polymerase require to create more DNA?
-dNTPs
-DNA template
-primer
-free 3’ OH group
_______ adds a primer that is complementary, RNA based, and about 10 bases long.
Primase
DNA polymerase proofreads by finding an error, which causes a pause and then initiates _________ _______.
exonuclease activity (3’-5’)
