Exam IV Flashcards
Which of the following is not true of bacterial replication?
A. replication is bi directional from the replication fork
B. To maintain short generation times, multiple origins of replication are present
C. The next round of replication can occur before the previous round has been completed
D. there is both a leading and lagging strand
B.
Which is true of plasmids?
A. they are usually linear
B. Their replication is tied to chromosome replication
C. they are usually less than 5% of the chromosome in size
D. contains genes of enzymes used in glycolysis
C
Which of the following is not a similarity between the replicative DNA polymerase and the RNA polymerase used in transcription?
A. both require nucleoside triphosphates
B. both polymerize in the 5’ to 3’ direction
C. both require templates
D. both require primers to initiate new strand synthesis
D.
Which is not used in regulation at the post-translational level by covalent modification?
A. Adenosine Diphosphate
B. Hydroxyl groups
C. Phosphate
D. Methyl groups
B.
T/F helicase plays a role in both DNA replication and transcription
False
T/F to inhibit bacterial transcription, Rho binds to the rut site within the RNA polymerase to physically detach it from the DNA
False
T/F In bacteria there tends to be one main sigma factor used for transcription but alternative factors are usually required for special circumstances
True
T/F all 64 codons of the genetic code encode for amino acids
False
T/F 5S rRNA is found in the large ribosomal subunit of both prokaryotes and eukaryotes
True
T/F Just as in eukaryotes, prokaryotes add sugars to their proteins within the golgi apparatus
False
T/F polysomes/polyribosomes are only observed in prokaryotes
False
T/F regulatory mechanisms that work after translation is completed are focused on regulating the activity of the enzyme rather than the amount present
True
T/F for the attenuation mechanism to regulate transcription it is imperative that the leader sequence is rich in adenine and uracil bases
False
to complete the replication of a bacterial chromosome, _________ proteins bind to _________ sites.
T/F these sites are found next to the origin of replication
tus
ter
False
Plasmids can replicate separately from the chromosome and often use the __________ method for replication
rolling circle
T/F Archaea use similar promoter sequences to bacteria
False
At the replication fork in Bacterial DNA replication, what keeps the strands from reverting to their more stable double stranded structure?
single-stranded binding proteins
What is the role of DnaA proteins in bacterial DNA replication
activates initiation of replication
How many RNA polymerases in each domain?
Bacteria and Archaea: 1
Eukarya: 3
T/F while the first tRNA binds to the P-site, all subsequent tRNAs will enter into the A site
True
T/F tRNAs are physically placed into position with the help of EF-Ts
false
What is the role of EF-G?
ribosome translocation
Name the antibiotic: targets the 50S ribosomal subunit
chloraphenicol, macrolides
T/F attenuation only occurs in prokaryotes. Explain
true
cannot happen in eukaryotes because transcription occurs in the nucleus
also attenuation requires polycistronic mRNA
