Exam IV

Question 1

Which of the following is not true of bacterial replication?
A. replication is bi directional from the replication fork
B. To maintain short generation times, multiple origins of replication are present
C. The next round of replication can occur before the previous round has been completed
D. there is both a leading and lagging strand

Answer 1

B.

Question 2

Which is true of plasmids?
A. they are usually linear
B. Their replication is tied to chromosome replication
C. they are usually less than 5% of the chromosome in size
D. contains genes of enzymes used in glycolysis

Answer 2

C

Question 3

Which of the following is not a similarity between the replicative DNA polymerase and the RNA polymerase used in transcription?
A. both require nucleoside triphosphates
B. both polymerize in the 5’ to 3’ direction
C. both require templates
D. both require primers to initiate new strand synthesis

Answer 3

D.

Question 4

Which is not used in regulation at the post-translational level by covalent modification?
A. Adenosine Diphosphate
B. Hydroxyl groups
C. Phosphate
D. Methyl groups

Answer 4

B.

Question 5

T/F helicase plays a role in both DNA replication and transcription

Answer 5

False

Question 6

T/F to inhibit bacterial transcription, Rho binds to the rut site within the RNA polymerase to physically detach it from the DNA

Answer 6

False

Question 7

T/F In bacteria there tends to be one main sigma factor used for transcription but alternative factors are usually required for special circumstances

Answer 7

True

Question 8

T/F all 64 codons of the genetic code encode for amino acids

Answer 8

False

Question 9

T/F 5S rRNA is found in the large ribosomal subunit of both prokaryotes and eukaryotes

Answer 9

True

Question 10

T/F Just as in eukaryotes, prokaryotes add sugars to their proteins within the golgi apparatus

Answer 10

False

Question 11

T/F polysomes/polyribosomes are only observed in prokaryotes

Answer 11

False

Question 12

T/F regulatory mechanisms that work after translation is completed are focused on regulating the activity of the enzyme rather than the amount present

Answer 12

True

Question 13

T/F for the attenuation mechanism to regulate transcription it is imperative that the leader sequence is rich in adenine and uracil bases

Answer 13

False

Question 14

to complete the replication of a bacterial chromosome, _________ proteins bind to _________ sites.
T/F these sites are found next to the origin of replication

Answer 14

tus
ter
False

Question 15

Plasmids can replicate separately from the chromosome and often use the __________ method for replication

Answer 15

rolling circle

Question 16

T/F Archaea use similar promoter sequences to bacteria

Answer 16

False

Question 17

At the replication fork in Bacterial DNA replication, what keeps the strands from reverting to their more stable double stranded structure?

Answer 17

single-stranded binding proteins

Question 18

What is the role of DnaA proteins in bacterial DNA replication

Answer 18

activates initiation of replication

Question 19

How many RNA polymerases in each domain?

Answer 19

Bacteria and Archaea: 1
Eukarya: 3

Question 20

T/F while the first tRNA binds to the P-site, all subsequent tRNAs will enter into the A site

Answer 20

True

Question 21

T/F tRNAs are physically placed into position with the help of EF-Ts

Answer 21

false

Question 22

What is the role of EF-G?

Answer 22

ribosome translocation

Question 23

Name the antibiotic: targets the 50S ribosomal subunit

Answer 23

chloraphenicol, macrolides

Question 24

T/F attenuation only occurs in prokaryotes. Explain

Answer 24

true
cannot happen in eukaryotes because transcription occurs in the nucleus
also attenuation requires polycistronic mRNA

Question 25

T/F in feedback inhibition, the end reaction for the synthesis of three similar proteins, ProD, ProP, ProR. This series of reactions are regulated for concert feedback inhibition mechanism. Based on what you learned in class - Dasherase, Prancerase, and rudolphase can be described as ___________________________

Answer 25

isoenzymes