Module 12 Flashcards

1
Q

4 advantages of electrophysiology

A

Can measure events on μs timescale
Can measure activity of single ion channel
Spatial resolution can be good
Can tell us how channels work

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2
Q

3 types of electrophysiology

A

Extracellula
Jntracellular
Patch

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3
Q

Eg of extracellular

A

EEG
ECG

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4
Q

What are the 3 configurations of patch clamp that all recording of single cells

A

cell attached,
Outside-out
Inside-out

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5
Q

What configuration allows recording of many channels

A

whole cell
Perforated patch (which could be considered the same thing or diff)

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6
Q

How do u make the patch clamp electrode

A

glass tube put inside heating element
Then each end pulled apart
Make sure to heat the end so the edges are smooth

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7
Q

What is added to outside of patch clamp electrode to insulate it

A

thick resin

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8
Q

Approx diameter of patch clamp electrode tip

A

1 micrometre

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9
Q

Micromanipulator function

A

converts large hand movements to small scale movements

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10
Q

What type of microscope most commonly used with patch clamp

A

flurorecesnce jksdhg microscope

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11
Q

How can a micromanipulator be made more specific than a manual one

A

use one with a hydrolic system

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12
Q

What is most expensive piece of equipment in patch clamp rig

A

patch amplifier
Amplifies the small currents produced by the patch clamp

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13
Q

What is AD board

A

analogue to digital converter

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14
Q

How is perforated patch a variation of whole cell recording

A

Instead of further suction to puncture the memb
Add pore forming antibiotic to pipette
Perforates memb
To form continuous path between pippete electrode thing and the cell

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15
Q

In inside out, why does cell need to be pulled out of bath soln briefly

A

so ends form seal with electrode instead of sealing together

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16
Q

Applications of cell attached

A

record currents of 1 or 2 channels under the electrode
Can see response to cell changes by the channel

17
Q

Applications of whole cell recordi g

A

record currents through channels across whole cell
Can see response to drugs added from outside into the cell
Can change solutions quickly, good for drug testing where u need to change conc fast

18
Q

Application of inside out recording

A

can manipulate things on intracellular face
And measure changes in channel

19
Q

Applications of outside out recordings

A

Can manipulate things on extracellular surface
Can change solutions daily quickly, but whole cell still preffered cuz outside in is hard to set up

20
Q

How can an agonist affect rate constants

A

agonist binding will increase k+1 (going forward)
And decrease k-1

21
Q

How do we find the rate constants

A

finding the mean of the closed time and open time

22
Q

What is conductance

A

ability of channel to move ions through pore
Can measure this to investigate properties of Channel pore

23
Q

How to find conductance

A

measure current at diff memb potentials

24
Q

what type of cell is two electrode voltage clamp used on

A

larger cells
usually in Xenopus oocyte experimetns

25
Q

what does each electrode do in a TEVC

A

a voltage electrode that measures the memb potential
and
a current electrode that injects current to maintain the clamp
A whole cell clamp would use a single electrode to do both of these things

26
Q

what type of test can be combined with patch clamp to find info about expression levels of diff channels

A

reverse transcriptase PCR

27
Q

when doing patch clamp on tissue slices, why is positive pressure force added to electrode

A

to get rid of debris and find the cell needed