Module 12 Flashcards
4 advantages of electrophysiology
Can measure events on μs timescale
Can measure activity of single ion channel
Spatial resolution can be good
Can tell us how channels work
3 types of electrophysiology
Extracellula
Jntracellular
Patch
Eg of extracellular
EEG
ECG
What are the 3 configurations of patch clamp that all recording of single cells
cell attached,
Outside-out
Inside-out
What configuration allows recording of many channels
whole cell
Perforated patch (which could be considered the same thing or diff)
How do u make the patch clamp electrode
glass tube put inside heating element
Then each end pulled apart
Make sure to heat the end so the edges are smooth
What is added to outside of patch clamp electrode to insulate it
thick resin
Approx diameter of patch clamp electrode tip
1 micrometre
Micromanipulator function
converts large hand movements to small scale movements
What type of microscope most commonly used with patch clamp
flurorecesnce jksdhg microscope
How can a micromanipulator be made more specific than a manual one
use one with a hydrolic system
What is most expensive piece of equipment in patch clamp rig
patch amplifier
Amplifies the small currents produced by the patch clamp
What is AD board
analogue to digital converter
How is perforated patch a variation of whole cell recording
Instead of further suction to puncture the memb
Add pore forming antibiotic to pipette
Perforates memb
To form continuous path between pippete electrode thing and the cell
In inside out, why does cell need to be pulled out of bath soln briefly
so ends form seal with electrode instead of sealing together