Microscopic anatomy of liver disease Flashcards
The image below shows normal liver microanatomy. What do each of the numbers labelled relate to?
1 - hepatocytes
2 - bile canaliculi
3 - bile duct
4 - branch of hepatic vein
5 - portal triad
6 - hepatic artery
7 - sinusoids
8 - central vein
What are the sinusoids of the liver?
- vascular spaces between hepatic plates
- blood flows from portal triad to central vein
What are the bile cannaliculi of the liver?
- sit between hepatocyes as these produce bile
- bile oves from central vein to portal triad
What are hepatic nodules?
- hexagon shapes of liver organisation
- central vein is in the middle
Acinar zones 1,2,3 are zones within the hepatic lodules. What is the relevence of the acinar zones?
- receiving blood progressively poorer in oxygen and nutrients close to central vein
- zone 3 will recieve blood that has been extracted of O2 and nutrients from zones 1 and 2
Acinar zones 1,2,3 are zones within the hepatic lodules. The acinar zones receive blood progressively poorer in oxygen and nutrients close to central vein. For example, zone 3 will recieve blood that has been extracted of O2 and nutrients from zones 1 and 2. Out of the 3 zones which is likley to suffer from ischemia and become damaged?
- zone 3
In the liver histology section image below, what do the colour arrows depict?
- red = hepatic artery
- green = bile duct
- blue = portal bein
In the liver histology section image below, what do the darker spots around the sinusoids depict?
- inflammatory cells
What is the space of Disse?
- a location in the liver between a hepatocyte and a sinusoid
- contains blood plasma
- can only been seen in pathophysiology
The sinusoidals of the liver are lined by a specific type or endothelial cell, what is this cell and what is the importance of this?
- fenestrated (like a sink hole with little holes)
- allows transfer of nutrients and plasma between hepatocytes and blood vessels
What are the immune cells of the liver?
- kupffer cells (macrophages)
Stellate cells of the liver, sit in the space of disse and are important for storage. What do they store?
- vitamin A
Hepatocytes contain a fine granuler eosinophilic cytoplasm. What colour does this stain on H&E staining?
- protein stain pink
- nucleus stains blue
Do hepatocytes contain glycogen?
- yes and can be stained for
- important for energy and blood glucose of liver
The image below is H&E stain of hepatocytes. What do the numbers 1, 2 and 3 denote?
1 - sinusoids
2 - nuclei of hepaticytes
3 - kupffer cells