Microscope Flashcards
An optical instrument designed to produce magnified visual or photographic images of small objects
microscope
The first vision instrument
reading stone
when was the reading stone invented
1000AD
An eyeglass maker experimented with multiple lenses which were placed in a tube
Zacharias Janssen and his dad
which century did Zacharias Janssen experimented with multiple lenses which were placed in a tube
16th century
father of microscopy
Anton Van Leeuwenhoek
father of microbiology
Anton Van Leeuwenhoek
First person to see and describe bacteria, protozoa, yeast, and sperm
Anton Van Leeuwenhoek
The first to use the basic microscope with 2 converging lens system
Robert Hooke
book that contains all the principles of the compound microscope
Micrographia
First man to discover the cellular structure of cork
Robert Hooke
who is under 17th century
Anton Van Leeuwenhoek
Robert Hooke
Invented the Phase-Contrast Microscope that is used for the study of colorless and transparent biological materials
Frits Zernike
when did Frits Zernike invented the phase-contrast microscope
1930
Frits Zernike invented the Phase-Contrast Microscope that is used for the study of ______and ______ biological materials
colorless
transparent
Invented the electron microscope
Ernst Ruska and Max Knoll
the electron microscope can magnify up to _______
1 million times
when did Ernst Ruska and Max Knoll invented the electron microscope
1931
identify the type of microscope:
natural light is the source to see the object
simple microscope
identify the type of microscope:
condenser not present and non - adjusting magnification
simple microscope
identify the type of microscope:
most commonly used
compound microscope
what are the 2 lens system used by compound microscope to magnify the image
objective lens
ocular lens
identify the type of microscope:
Can have up to 4 objective lenses of different magnifications
compound microscope
identify the type of microscope:
The image that appears is three-dimensional
dissecting microscope
dissecting microscope is used to study the _______ of the solid specimen
surface
dissecting microscope is also called
stereoscope
stereoscopic microscope
identify the type of microscope:
An instrument that permits the study of internal cell structure without the need to stain
phase- contrast microscope
identify the type of microscope:
used to count platelets
phase-contrast microscope
identify the type of microscope:
contrasting-enhancing technique used to identify substances such as crystals in urine and other body fluid
polarized light microscope
identify the type of microscope:
it uses a special condenser
dark field microscope
identify the type of microscope:
a contrasting-enhancing optical technique
phase- contrast microscope
identify the type of microscope:
dark field microscope is helpful in microbiology in the identification of ______
spirochetes
identify the type of microscope:
Uses a beam of electrons as a source of illumination
electron microscope
identify the type of microscope:
the dark field microscope uses special condenser to create ______
dark background
identify the type of microscope:
used for demonstration of very thin bacteria
dark field microscope
true or false
phase contrast microscope instrument that permits the study of internal cell structure without the need to stain
true
the living specimen in electron microscope is destroyed by the _______
high radiation of electron beam
what are the 2 types of electron microscope
scanning electron microscope (SEM)
transmission electron microscope (TEM)
identify the type of electron microscope:
produce a 3-D image of specimen’s surface features
scanning electron microscope
identify the type of electron microscope:
produce a 2-dimensional image of specimen
transmission electron microscope
transmission electron microscope provides detailed structure of______
internal surface
important parts of a compound microscope:
also known as ocular
eyepiece
eyepiece has a ____ magnification
10x
important parts of a compound microscope:
between the ocular and objective lens
body tube
important parts of a compound microscope:
it is where the microscope is held at
arm
important parts of a compound microscope:
allows for easy rotation from one objective lens to another
nosepiece
important parts of a compound microscope:
for magnification
objective lenses
important parts of a compound microscope:
supports the prepared microscope slide
stage
important parts of a compound microscope:
to hold the specimen in place
stage clips
important parts of a compound microscope:
focus light into the specimen
condenser lens
important parts of a compound microscope:
used to adjust the magnification of objective lens (zoom in and out)
coarse adjustment knob
important parts of a compound microscope:
to adjust the working distance between the stage and objective lenses
fine adjustment knob (for HPO and OIO)
important parts of a compound microscope:
light source
lamp
important parts of a compound microscope:
change the intensity of light
diaphragm
what are the 2 types of diaphragm:
disk diaphragm
iris diaphragm
identify the type of diaphragm:
used in old microscope
disk diaphragm
identify the type of diaphragm:
regulates the amt of light by adjusting the lever
iris diaphragm
what are the types of brightfield illumination
critical illumination
koelher illumination
critical illumination, the light source is focused at the _____
specimen
koehler illumination, the light illumination is focused at the ________
condenser aperture diaphragm
the equation that descries the relationship of the angle of light that the objective collects and the refractive index of the medium between the specimen and the objective
numerical aperture
NA
numerical aperture
it regulates the balance between
contrast and resolution
numerical aperture
what are the 4 magnification
scanning
low power objective
high power objective
oil immersion
magnification color code of LPO
yellow
magnification color code of scanning
red
magnification color code of HPO
blue
magnification color code of OIO
white
true or false
carry the microscope with one hand
false -carry with two hands
true or false
use the fine adjustment when working with the higher objectives
true
When not in use for an extended period of time, the microscope should be …
covered or protected from dust
what should you do before and after using the microscope
inspect the component parts
avoid placing fingers on the ….
lens surfaces
true or false
you are able to use water to clean the lenses
false
important parts of a compound microscope:
to adjust the the stage height
stage adjustment knob
what is the total magnification for scanning electron microscope
20 000x
what is the total magnification for transmission electron microscope
20 million times
compound microscope is also known as
biological microscope
single lens is called
loupe
