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Microbiology > MicroLab Quizzes > Flashcards

MicroLab Quizzes Flashcards

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0
Q

What happens to the light as you move to more powerful objectives?

A

Gets dimmer

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1
Q

What does it mean if objectives are parfocal?

A

Partially focused

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2
Q

What happens to field of view as you move to more powerful objectives?

A

Gets smaller

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3
Q

What is the only thing you should use to clean the ocular or objective lenses?

A

Lens paper

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4
Q

Objective band color when the objective is on scan?

A

Red

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5
Q

When the objective color band is yellow what is the objective on?

A

Low power

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6
Q

Objective band color when the objective is on high power?

A

Blue

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7
Q

Objective band color when the objective is on oil immersion?

A

White

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8
Q

What is the ocular magnification when the objective is on low power?

A

10x

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9
Q

What is the ocular magnification when the objective is on oil immersion?

A

10x

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10
Q

What is the objective magnification when the objective is on scan?

A

4x

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11
Q

What is the objective magnification when the objective is on low power?

A

10x

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12
Q

Which objective is at 400x total magnification?

A

High power

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13
Q

What happens when the objectives you see move to more powerful objectives?

A

Gets larger

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14
Q

Which objective is at 1000x total magnification?

A

Oil immersion

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15
Q

What is used to clean the microscope lenses?

A

lens paper

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16
Q

If you saw something a furry colony, what type of organism is it?

A

mold

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17
Q

True or False: normally petris dishes are placed in the incubator upside down.

A

true

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18
Q

Where are petri dishes suppose to be labeled?

A

on the bottom where the agar is

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19
Q

true or false: normal hand washing removes the bacteria from your skin, so nothing grew on the petri dish

A

false

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20
Q

What temperature is the incubator set as in degrees Celcius?

A

37*

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21
Q

How much inoculum (bacteria) do you use when making a smear from a broth culture?

A

6-8 loopfuls

22
Q

How much inoculum (bacteria) do you use when getting it from a slant?

A

pinpoint

23
Q

List 2 reasons why you fix a smear.

A
  • to keep the bacteria & stain in place
  • Shrink the cell
  • kill bacteria
24
Q

List an example of a basic stain

A

methylene blue

25
Q

What kind of bond forms between the basic stain and the bacteria?

A

ionic bond

26
Q

Give an example of an acidic stain.

A

congo red

27
Q

What does an acidic stain do?

A

stain the background

28
Q

Give an example of a basic stain?

A

maneval stain

29
Q

What does a basic stain do?

A

stain the cell

30
Q

How did the capsule look after the capsule stain procedure?

A

clear/white

31
Q

Why do you never heat fix a capsule stain?

A

it shrinks the cell

32
Q

What did we use in the capsule stain procedure to make the bacteria stick to the slide?

A

sheep serum

33
Q

List 2 bacteria we used for Gram staining, designate their Gram (+/-) and their final color

A

Escherichia coli: Gram (-): pink

Staphylococcus aureus: Gram (+): purple

34
Q

What is the counter stain in the Gram stain procedure?

A

Safranin

35
Q

What is the primary stain in the Gram stain procedure?

A

Crystal violet stain

36
Q

What is the primary stain in the spore stain?

A

malachite green

37
Q

What is the counter stain in the spore stain?

A

safranin

38
Q

In the spore stain, the spore appears as

A

green ovals

39
Q

What color are acid-fast bacteria after staining?

A

reddish-purple

40
Q

What is the primary stain in the acid-fast stain?

A

carbol fuschin

41
Q

What is the counter stain in teh acid-fast stain?

A

methylene blue

42
Q

List a species of bacteria that would be spore-forming.

A

Bacillus subtilis,

43
Q

List a disease cased by a spore-forming bacterium

A

botulism

44
Q

List a species of bacteria that would be acid-fast.

A

Mycobacterium smegmatis

45
Q

List a disease caused by an acid-fast bacterium

A

tuberculosis (TB)

46
Q

What procedure could we perform that would separate mixture of different kinds of bacteria?

A

streak plate isolation

47
Q

What does a colony start out as?

A

1 bacterium that divides multiple times and isolates itself from other bacteria

48
Q

What are 2 problems with cultures when working with unknowns?

A
  1. contamination –>asceptic transfer

2. Over growth & denature

49
Q

When you get your unknown, where will you keep your reserve stock?

A

fridge

50
Q

What does a positive catalase test look like?

A

bubbles

51
Q

What is the product that you are seeing when you have a positive catalase test?

A

O2

52
Q

What is the substrate for catalase?

A

H2O2

53
Q

The catalase test distinguides between 2 genera of Gram (+) bacteria. List them and then designate how they test for catalase.

A

Streptococcus –> catalase (-)

Staphylococcus –> catalase (+)

Microbiology (20 decks)

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