Micro EX 1: Microbial Growth Flashcards
pH formula
pH= -log[H]
most bacteria thrive between what range
6-8
most fungi thrive between what range
5-6
Describe acidophiles
likes acid
Define Ketoacidosis
ketosis in body increases; prone to fungal growth
ex: yeast infection
Human normal temperature in Celsius
37 degrees C
everything a minimum and maximum temperature; if it was below min? if was above max?
below minimum: dont die
above maximum: die
Define Psycho
likes the cold
Psychophiles
10-15 degrees C
Psychotrophs
0-30 degrees C
Mesophiles (normal flora & pathogens)
10-50 degrees C
Thermophiles (soil bacteria & compost)
40-70 degrees C
Hyperthermophiles (hot springs, ocean vents, volcanoes)
65-110 degrees C
What are the 3 Physical Growth Requirements?
pH, Osmotic Pressure, Temperature
Osmotic Pressure: lots of particles less water
Hypertonic
Examples of hypertonic
NaCl (salt water) & honey
Osmotic Pressure: equal amounts of particles & water
Isotonic
Define Plasmolysis
they shrink but not cell wall
What thrives in Hypertonic solution?
Halophiles & Facultative Halophiles
What can live w/ or w/o salt water?
Facultative Halophiles
Examples of Isotonic
0.9% NaCl & 5% glucose
Osmotic Pressure: little particles more water
Hypotonic
In hypotonic solution what prevents the bacteria from bursting? Name 1 example
cell wall
Ex: Myoplasma
what are the 3 chemical requirements for microbial growth
Carbon, Oxygen, Nitrogen
What makes their own food from CO2?
autotrophs
What are the 2 types of autotrophs?
photoautrophs & chemoautotrophs
Which autotrophs uses sunlight for energy?
photoautrophs
Which autotrophs uses chemical reactions for energy?
chemoautrophs
What need organic molecules (carbon) for food?
Heterotrohphs
Define Nitrogen fixed bacteria
N-H or N-O to make usable compounds by plants
Define Nodules in Roots of Legumes
symbiotic Nitrogen fixing bactera; Nitrogen gas converted into ammonia
Define Crop Rotation
replace different nutrients back into soil
Define Fertilization Production
crops–> ammonia nitrates–> animals–> denitrification–> atmospheric nitrogen fixation–> nitrogen fixing bacteria–> ammonia nitrates
Describe Obligate Aerobe
needs O2
Describe Micro Aerophilic
needs small amounts of O2
Describe Facultative Anaerobe
thrive w/ or w/o O2
Describe Obligate Anaerobe
don’t need O2
Example of Obligate Aerobe
Bacillus
Example of Micro Aerophilic
Helicobacter pylori
Example of Facultative Anaerobe
E. coli
Example of Obligate Anaerobe
Clostridium
What are the 4 stages of Bacterial Growth Curve?
- Lag Phase
- Log Phase
- Stationary Phase
- Death Phase
Bacterial Growth Curve: exponential growth in population
Log Phase
Bacterial Growth Curve: preparation for population growth
Lag Phase
Bacterial Growth Curve: population decreasing logarithmically
Death Phase
Bacterial Growth Curve: period of equilibrium for microbial death balance for production of new cells
Stationary Phase
Describe Culture
actual microbes; pure culture: 1 organism growing only
Describe Media
where bacteria live on; comes in liquid(broth) or solid(agar)
Describe the origin of Agar
derived from red algae
Define Agar
thickening agent
What type of Media do we use in lab? Name 2 examples
Complex Media; tryptic soy agar & nutrient broth
What type of Media do we not use in lab & use for high dollar research?
Chemically defined Media
Define Fastidous Microbes
hard to grow; needs lots of organic foods
Example of a Fastidous Microbe
Neisseria gonorrhoeae
List the Prokaryotic Structures
Capsule, cell wall, cytoplasmic membrane, ribosome, pili, flagella, & Nucleoid