Microbiology Flashcards

1
Q

What is the range of size for a prokaryote

A

0.2 μm > 700 μm diameter

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2
Q

What is the range of size for a eukaryote

A

10 μm > 200 μm diameter

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3
Q

What is a protozoa

A

A eukaryote - animal-like
No cell wall or chlorophyll
Ingest particulate food

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4
Q

What is algae

A

A eukaryote - plant-like
Rigid cell wall
Has chlorophyll
May be multicellular

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5
Q

What is fungi

A
Eukaryote
Rigid cell wall
No chlorophyll
Uni- or Multi-cellular
Secrete enzymes that degrade many complex structures
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6
Q

What is bacteria

A

Prokaryote
Rigid cell wall
Unicellular
No chlorophyll but may be photosynthetic
May secrete enzymes that degrade complex structures
Divided into eubacteria and archaea

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7
Q

What are viruses

A

Simple protein-like structure
Metabolically inert
Infect ALL kingdoms

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8
Q

Why is cell morphology important

A

Identifies shape, structure, form, size of cells

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9
Q

What are examples of the selective forces that maybe involved in setting the morphology

A

Optimisation for nutrient uptake (small cells and those with high surface-volume ratio)
Swimming motility in viscous environment or near surfaces (helical or spiral-shaped cells)
Gliding motility (filamentous bacteria)

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10
Q

Do eukaryotes and/or prokaryotes have a nuclei

A

Only eukaryotes

Prokaryotes have nuclear material

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11
Q

Do eukaryotes and/or prokaryotes have membrane bound organelles and compartments

A

Eukaryotes have membrane bound organelles and compartments

Prokaryotes have no membrane bound intracellular compartments

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12
Q

What are the fundamental differences between archaea and eubacteria

A

Distinct 16S rRNA sequences
Cell wall composition
Lipid content of membranes
Transcription and translation processes

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13
Q

Where is cyanobacteria present and how does it gain energy

A

Marine and freshwater microflora

Photosynthesis

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14
Q
Are proteobacteria (purple bacteria) positive/negative
Give examples
A

Gram-negative bacteria
E. coli
Salmonella typhimurium

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15
Q

Examples of gram-positive bacteria

A

Bacillus anthracis

Staphylococcus aureus

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16
Q

When mixed with dyes what colour does gram-positive bacteria turn, and what is the colour after washed

A

Purple

Remains purple

17
Q

What colour is gram-negative bacteria after the purple stain is washed off

A

Does not retain purple colour - becomes pink - counterstain

18
Q

What is a bacterial endospore

A

Dormant structure
Can persist for long periods
Dehydrated - no biochemical reactions occur
Germination and outgrowth lead to new vegetative cell