Micro Lab 3 Flashcards
1
Q
Selective Media
A
- allows the growth of some organisms
- inhibits the growth of others
2
Q
differential media
A
- differentiates between closely related organisms or groups
- based on biochem characteristics
3
Q
sheep blood agar
A
- differential
- for bacterial hemolysins
4
Q
TSA
A
- non-selective
- non-differential
5
Q
MacConkey
A
- selective for gram negatives
- differential for ability to ferment lactose
6
Q
how MacConkey selective for gram negatives?
A
- bile salts and crystal violet inhibit growth of gram positive bacteria
7
Q
fermenter of lactose color
A
- pink
8
Q
mannitol salt agar
A
- selective for Staph
- differential for pathogenic staph
- ferment mannitol
9
Q
color of fermented mannitol
A
- yellow
10
Q
triple sugar iron
A
- tests for sugar fermentation, sulfur reduction, and gas production simultanously
11
Q
glucose fermentation TSI
A
- rapid glucose fermentation decreases pH and turn media yellow
- red stant, yellow butt
- K/A
12
Q
fermentation of glucose and lactose/sucrose TSI
A
- yellow slant and butt
- A/A
13
Q
sulfur reducers TSI
A
- results in black precipitate
14
Q
non sugar fermenter TSI
A
- red stand and red butt
- utilized peptones only
- K/K
15
Q
MIO
A
- motility
- ornithine decarboxylase
- indole
