MIC Flashcards
1
Q
ESBLs hydrolyze…
A
- penicillins
- cephalosporins
- monobactams (aztreonam)
2
Q
sensitive to clavulinic acid
A
ESBL
KPC
3
Q
resistant to clavulinic acid
A
ampC
NDM-1
4
Q
sensitive to EDTA
A
NDM-1
5
Q
ampC hydrolyzes…
A
- penicillins
- cephalosporins
- cephamycins (cefoxitin, cefotetan, cefmetazole)
- monobactams (aztreonam)
6
Q
NDM-1s hydrolyze…
A
- penicillins
- cephalosporins
- cephamycins (cefoxitin, cefotetan, cefmetazole)
- carbapenems
7
Q
KPCs hydrolyze…
A
- penicillins
- cephalosporins
- cephamycins (cefoxitin, cefotetan, cefmetazole)
- carbapenems
- monobactams (aztreonam)
8
Q
BLase sensitive to cephamycins
A
ESBLs
9
Q
BLase sensitive to monobactams (aztreonam)
A
NDM-1s
9
Q
BLase sensitive to carbapenems
A
ESBLs
ampCs
10
Q
BLase sensitive to cefepime (4th gen cephalosporin)
A
ampC
11
Q
B-lactam family of antibiotics affected by BLases (5)
A
penicillins
cephalosporins
cephamycins
carbapenems
monobactams
12
Q
test for carbapenemase
A
Modified carbapenem inactivation method
13
Q
explain modified carbapenem inactivation method
A
- 10-μg meropenem (MEM) disk is incubated for 2 h in a suspension of a carbapenemase-producing microorganism
- Carbapenem in the disk is degraded by the carbapenemase
- Disk is removed from the suspension and placed onto a Mueller-Hinton agar (MHA) plate seeded with a suspension of a carbapenem-susceptible indicator organism (E. coli ATCC 25922)
- Following overnight incubation, the zone of inhibition is measured to determine whether the carbapenem had been hydrolyzed (growth of the indicator organism close to the disk), or is still active (a large zone of inhibition around the disk)
14
Q
KPC/CRE inhibited by…
A
clavulinic acid and tazobactam
