10. Anaerobes Flashcards
GPRs (5)
Clostridium
Actinomyces
Cutibacterium
Lactobacillus
Bifidobacterium
GNRs (4)
Bacteroides
Fusobacterium
Prevotella
Porphyromonas
GNC (1)
Veillonella
GPCs (4)
Finegoldia
Peptostreptococcus
Peptoniphilus
Streptococcus (micro)
Clostridium
catalase
resistance
cat =
colistin R
no WBC in stain
C. perfringens
C. —– rarely makes spores
perfringens
C. perfringens disease
gas gangrene
necrotizing fasciitis
food poisoning
soft tissue infection
bacteremia (elderly)
gray, flat rhizoid colony that spreads, but doesn’t swarm
C. perfringens
double Beta
C. perfringens
Alpha & Theta toxins
C. perfringens
tennis racket
C. tetani
C. tetani indole
indole +
toxin of C. tetani
tetanospasmin
Beta swarming colony
C. tetani
dx by s/s, not culture
C. tetani
toxins A & B
C. difficile
culture not performed but colony is chartreuse and has horse odor
C. difficile
isolation from blood = colon cancer
C. septicum
Medusa head, swarms
C. septicum
C. septicum indole
indole =
Nonswarming clostridium; end of pregnancy infection
C. sordellii
end of pregnancy
C. sordellii
C. sordellii indole, urease
indole +
urease +
Actinomyces GS
long, thin, branched, beaded GPR
short, clubbed, pleio, palisading GPR
Cutibacterium
C. acnes catalase indole
cat +
indole +
long, thin, chain forming GPR
lactobacillus
ppt alpha/gamma
intrinsicallyl Vanc resistant
Lactobacillus
“Y” dog bone
Bifidobacterium
dental caries
small white colony
Bifidobacterium
Bifidobacterium cat indole nitrate
catalase =
indole =
nitrate =
enzyme made by all anaerobic GNRs
B-lactamase
pleio/CB with or without vacuoles
Bacteroides fragilis
esculin +
B. fragilis
B. fragilis resistance
Pen
Vanc
Kanamycin
Colistin
large gray wet speckled
B. fragilis
B. fragilis infections
below the waist
obligate anaerobes, NOF
Fusobacterium
Fusobacterium resistance
Vanc
fluoresce chartreuse
Fusobacterium
rancid butter, butyric acid
Fusobacterium
Fusobacterium indole
indole +
long thin fusiform rod
F. nucleatum
F. nucleatum infections
above the waist, especially lungs
speckled, breadcrumbs, smooth gray/white
F. nucleatum
Differentiate the Fusobacteriums
F. nucleatum: lipase =
F. necrophorum: lipase +
bizarre pleio
F. necrophorum
F. necrophorum infections
Vincent’s angina/trench mouth
Lemierre’s disease
Prev/Porph GS
GNCB/pleio
tan/black colonies on media with blood
Prev/Porph
red fluorescence
Prev, Porph, Veill
Prevotella melaninogenica resistance
Vanc
Kanamycin
Prev/Porph infections
above the waist
oral flora
Porphyromonas resistance
Kanamycin
Colistin
Differentiate Prev/Porph
Prevotella: grows on LKV
Porphyromons: ng on LKV
GNCCL
Veillonella parvula
Veillonella parvula resistance
Vanc
Veillonella parvula LKV
ng
Veillonella parvula infections
mixed
above/below waist
Veillonella nitrate
nitrate +
GPC resistance pattern
S to Vanc
V to colistin
R to kanamycin
25% anaerobic infections
GPC
most pathogenic anaerobic GPC
Finegoldia magna
Finegoldia infections
endocarditis
joint
respiratory
prostheses
diabetic food wound
larger than average GPC - mimics Staph
Finegoldia magna
Finegoldia magna catalase indole SPS
catalase =
indole =
R to SPS
Peptostreptococcus anaerobius infections
mixed
abdomen, GU, respiratory, oral
elongated cocci in PR/CH
Peptostreptococcus anaerobius
sweet odor, isocapric acid
Peptostreptococcus anaerobius
Peptostreptococcus anaerobius SPS
S to SPS
Peptoniphilus asaccharolyticus infections
below the waist
especially female reproductive system
stain poorly, appear as GNC
Peptoniphilus asaccharolyticus
Peptoniphilus asaccharolyticus indole
indole +
Microaer Strep requirement
10% CO2
Micro Strep formerly…
S. milleri
Micro Strep colony
ppt Beta
butterscotch odor
micro Strep PYR
PYR =
(Group A is PYR +)
brain abscesses
S. intermedius
abdomen, head and neck infections
Micro Strep
antibiotics above diaphragm
clindamycin
antibiotics below diaphragm
metronidazole
does not affect aerobes
antibiotics used for multiple sites
B-lactams/combos
carbapenems
moxifloxacin
Can tolerate exposure to 2-8% oxygen for 30 minutes to a few hours
moderate obligate
moderate obligate groups
Bacteroides fragilis group
Prevotella-Porphyromonas groups
Fusobacterium nucleatum
Clostridium perfringens
2 factors that affect growth of anaerobes
Inhibitory effect of oxygen and its toxic derivatives
Oxidation-reduction potential of media
Eh
Measurement of environment’s ability to lose (oxidize) or gain (reduce) electrons
ideal specimen
whole tissue or aspiration with needle and syringe
redox indicator
Resazurin
clues to presence of anaerobes
1) close to mucosa
2) necrotic/low bloodflow
3) foul odor
4) gas in tissues
5) sulfur granules
6) black discoloration
7) fluorescence
etc
Pmel requirements
vitamin K
hemin
produces vitamin K
Saur
reducing ingredients in RBAP
palladium chloride, L-cysteine HCL and dithiothreitol
main enriched and selective agar
Laked kanamycin-vancomycin agar (LKV)
BBE ingredients
esculin, bile, hemin, vitamin K and gentamicin
ideal anaerobic atmosphere
80-90% nitrogen
5% hydrogen
5-10% carbon dioxide
how do self contained agars work?
Media contains enzymes which reduces oxygen in agar and space above medium
anaerobic generating system chemical reactions
Creates hydrogen and carbon dioxide
Hydrogen combines with oxygen to form water in the presence of a palladium catalyst
Desiccant absorbs the water produced
explain role of charcoal filter in anaerobic chamber
Charcoal filter absorbs hydrogen sulfide and other metabolic by-products which can inactivate the palladium catalyst
when to examine plates
48 hours, 3, 4 and 5 days
how to perform aerotolerance test
Sub to one-quarter section of CHOC and an entire RBAP
read at 48 hours
wound cultures take —- days minimum
5
when to do full ID and susceptibility
Pure culture of one anaerobe or a sterile body site or a clinically significant site
red fluorescence is due to…
except for…
breakdown of hemoglobin to heme
Veillonella
gas-liquid chromatography detects…
Volatile fatty acids
Alcohols
Non-volatile organic acids
grow organism in —— before gas-liquid chromatography
glucose broth
solubilizes end products of glucose metabolism
ether/chloroform
catalase +
C. acnes