8. Nonfermenting GNRs Flashcards
1
Q
yellow pigment (4)
A
E. meningoseptica
S. maltophila
B. cepacia
P. stutzeri
MEN MAUL STU and CEP
2
Q
blue-pink on MAC
A
A. baumanii
3
Q
wrinkled colony
A
P. stutzeri
B. pseudomallei
4
Q
Grape odor
A
P. aeruginosa
5
Q
Nonmotile Nonferms (4)
A
A. baumanii
Moraxella
E. meningoseptica
B. mallei
MORE MEN with MALlets and BOWS are nonmotile
6
Q
Ox = (2)
A
A. baumanii
S. maltophila
7
Q
H2S +
A
S. putrifaciens
8
Q
Lysine + (2)
A
B. cepacia
S. maltophila
9
Q
DNase +
A
S. maltophila
10
Q
has pyocyanin
A
P. aeruginosa
11
Q
has pyoverdine
A
P. aeruginosa
P. fluorescens
12
Q
Indole +
A
E. meningoseptica
13
Q
Ammonia odor
A
S. maltophila
14
Q
Dirt odor (2)
A
B. cepacia
B. pseudomallei
15
Q
Assacharolytic
A
Moraxella
16
Q
A. baumanii GS
A
GNCB
17
Q
Moraxella GS
A
GNCB
18
Q
Distinguish Pseudomonas based on temperature
A
Paer will grow at 42°, and Pflu will not
Paer will not grow at 4°, and Pflu will
19
Q
A. baumanii SBA
A
“G
gray
smooth wet”
20
Q
B. cepacia SBA
A
yellow
21
Q
B. mallei SBA
A
wet/gray
22
Q
B. pseudomallei SBA
A
“cream/tan
wrinkled”
23
Q
E. meningoseptica SBA
A
yellow
24
Q
P. aeruginosa SBA
A
“B/G
dry or mucoid
pigmented
scaled”
25
P. fluorescens SBA
"med
wet/gray"
26
P. stutzeri SBA
"dry/wrinkled
yellow"
27
S. putrifaciens SBA
"orange/tan
mucoid"
28
S. maltophila SBA
yellow
29
May oxidize lactose to give false + on MAC
A. baumanii
B. cepacia
B. pseudomallei
30
Ox + nonferm GNRs (5)
Burkholderia
Elizabethkingia meningoseptica
Moraxella osloensis
Pseudomonas
Shewenella putrifaciens
31
Ox variable
Burkholderia cepacia
32
Test unique to E. meningoseptica
Indole
33
Tests unique to S. putrifaciens
H2S
Ornithine
34
Test unique to S. maltophila
DNase
35
OF G/L/M
+/+/+
(2)
A. baumanii
B. pseudomallei
36
OF G/L/M
+/V/V
(1)
B. cepacia
37
OF G/L/M
+/=/+
(4)
E. meningoseptica
S. maltophila
P. aeruginosa
P. stutzeri
38
OF G/L/M
=/=/=
(2)
Moraxella
S. putrifaciens
39
OF G/L/M
+/=/=
(1)
P. fluorescens
40
Nitrate + (4)
Elizabethkingia meningoseptica
Moraxella osloensis
Pseudomonas aeruginosa
Pseudomonas stutzeri
MEN with MORE Nitrate eat PEARS with STU
41
Arginine + nonferms (4)
Burkholderia mallei
Burkholderia pseudomallei
Pseudomonas aeruginosa
Pseudomonas fluorescens
42
Ornithine + (1)
S. putrifaciens
43
aerobic oxidation of CHO pathway name
Entner-Duodoroff pathway
44
Clues that an organism may be a nonferm GNR (6)
1) no CHO ferm (TSI, KIA)
2) Ox +
3) Poor growth on MAC
4) Odors, colors, morphologies
5) Antibiotic resistance
6) No positive rxns in tests used for Enterobacteriaceae
45
OF tubes ingredients
0.2% peptones
1% CHO
bromothymol blue
46
most common 3 nonferm isolates
P. aeruginosa, A. baumannii, S. maltophilia
47
motility test temperature for nonferms
25°
48
Brownian movement
natural movement of organisms on a wet mount
not motility
49
2 additional pigments that nonferms can have
pyomelanin
pyorubin
50
color of control tube for nonferm decarboxylase reactions
dirty gray
no glucose ferm, not yellow
51
decarb tests for nonferm need to be incubated ----- days to confirm a true =
5
52
yellow, water-soluble pigment
function
pyoverdine
iron acquisition
53
nonferm GNRs generally like what environments?
wet, humid
54
pyocyanin function
toxin that kills mammalian and bacterial cells
55
Paer virulence factors (10)
LPS
Hemolysins
Exotoxins
Cytotoxins
Proteolytic enzymes
Capsule and biofilm
Pili
Leukocidin
Pyocyanin
Intrinsically MDR
56
Paer (does/does not) colonize healthy people
does not
57
risk factors for Paer colonization (6)
Burns
Cystic fibrosis (CF)
Ventilator use, tracheostomy
Organ Transplants
Leukemia
Neutropenia
IV drug addiction
58
Paer clinical significance
Wound infections
Folliculitis
UTI
LRT infection (CF, COPD)
septicemia
keratitis
osteomyelitis
fingernail bed infections
59
Problematic organisms in CF
Paer
Bcep
Smal
60
polysaccharide reponsible for mucoid Paer
alginate
61
rapid ID of Paer (5)
NLF GNR
Beta
Oxidase +
Fruity odor of grapes/warm corn tortillas
Metallic sheen and/or Blue-green pigment
62
Paer is so MDR, ------ is used to tx CF patients with it
high dose aerosolized tobramycin
oral azithromycin
63
Pflu optimal growth temp
25°
64
Pflu associated with...
transfusion acquired septicemia
spoilage of refrigerated food
65
Only difference between Pflu and P. putida
Pflu will grow at 4°
66
Nonfluorescent Pseudomonas
P. stutzeri
67
Bioterror agents
B. anthracis
B. pseudomallei
B. mallei
68
B. cepacia complex includes ---- species
18
69
started as a plant pathogen
Bcep
70
Known for pseudo-infections associated with contaminated equipment, disinfectants and soaps
Bcep
71
Bcep main clinical significance
complication of CF
72
Bcep in CF leads to death via...
lung failure
73
3 selective agars for Bcep
BC agar
OFPBL agar
BCSA
74
other disease that Bcep can complicate
leads to...
chronic granulomatous disease (CGD)
recurrent life-threatening bacterial and fungal infections
75
must use 2 methods of ID
Bcep in CF pt
76
Differentiate Bcep from Smal as they are both Lysine +
oxidase
77
Drug of choice for Bcep, which is extremely MDR
SXT
78
used to degrade agent orange
Bcep
79
Found in southeast Asia, causes melioidosis
B. pseudomallei
80
Must use antibiotics that penetrate WBC and treat for up to 6 months
Can hide and cause infection decades later
Bpse
81
Differentiates Bpse from Pstut as they both have wrinkled colonies
OF lactose
Arginine
82
Commercial systems can mis-ID ------- as Pseudomonas
B. pseudomallei
83
Causes glanders
Bmal
84
Primarily a respiratory disease of equines (horses, mules, donkeys)
Bmal (glanders)
85
4 forms of glanders
Acute localized infection
Septicemic illness
Acute pulmonary infection
Chronic suppurative disease
86
High fatality rate
90-95% without treatment
50% with antibiotic therapy
B. mallei
87
Used as biowarfare agent in WW1
Bmal
88
May only be pathogenic when it acts in combination with other pathogens present
Smal
89
Smal clinical significance
Pneumonia
Catheter related bacteremia
UTI
Wound infection
Sign that CF pt is deteriorating
90
Septicemia with metastatic abscesses on skin, in lungs and other organs
Vietnamese time bomb
Bpse
91
Differentiates Smal from Enterobacteriaceae
TSI/KIA
92
Drugs of choice for Smal
SXT
Colistin
Polymixin B
93
Aquatic or marine environment but does not require NaCl for growth
Shewanella
94
Strong gross fishlike odor
Shewanella
95
Highly pathogenic for premature infants
E. meningoseptica
96
Emen is sensitive to...
antibiotics regularly used for GPs (vanc, B-lactams, SXT, rifampin)
97
Moraxella will appear more like rods after...
exposure to antibiotics
98
M. lacunata clinical significance
conjunctivitis and keratitis
99
Differentiates M. lacunata from Mcat
Mlac is penicillin susceptible
100
A. baumanii mimics ---- on GS
Neisseria, Mcat, Staph
101
Differentiates A. baumanii from Neisseria
Oxidase
102
Abau's favorite CHO
lactose
103
Leading cause of ventilator-associated pneumonia
Abau
104
traumatic injuries in individuals after natural disasters such tornadoes and tsunamis
Abau
105
Abau's nicknames
great impersonator
Iraqibacter
Gram-negative MRSA
106
mini biochemicals for nonferms
RapID NF Plus System
107
RapID NF Plus System incubation
4 hours
108
Nonfermenting GNR genera (7)
Acinetobacter
Burkholderia
Elizabethkingia
Moraxella
Pseudomonas
Shewanella
Stenotrophomonas
