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Mercy CLS 2: Microbiology > 8. Nonfermenting GNRs > Flashcards

8. Nonfermenting GNRs Flashcards

1
Q

yellow pigment (4)

A

E. meningoseptica
S. maltophila
B. cepacia
P. stutzeri

MEN MAUL STU and CEP

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2
Q

blue-pink on MAC

A

A. baumanii

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3
Q

wrinkled colony

A

P. stutzeri
B. pseudomallei

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4
Q

Grape odor

A

P. aeruginosa

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5
Q

Nonmotile Nonferms (4)

A

A. baumanii
Moraxella
E. meningoseptica
B. mallei

MORE MEN with MALlets and BOWS are nonmotile

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6
Q

Ox = (2)

A

A. baumanii
S. maltophila

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7
Q

H2S +

A

S. putrifaciens

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8
Q

Lysine + (2)

A

B. cepacia
S. maltophila

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9
Q

DNase +

A

S. maltophila

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10
Q

has pyocyanin

A

P. aeruginosa

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11
Q

has pyoverdine

A

P. aeruginosa
P. fluorescens

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12
Q

Indole +

A

E. meningoseptica

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13
Q

Ammonia odor

A

S. maltophila

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14
Q

Dirt odor (2)

A

B. cepacia
B. pseudomallei

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15
Q

Assacharolytic

A

Moraxella

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16
Q

A. baumanii GS

A

GNCB

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17
Q

Moraxella GS

A

GNCB

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18
Q

Distinguish Pseudomonas based on temperature

A

Paer will grow at 42°, and Pflu will not

Paer will not grow at 4°, and Pflu will

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19
Q

A. baumanii SBA

A

“G
gray
smooth wet”

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20
Q

B. cepacia SBA

A

yellow

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21
Q

B. mallei SBA

A

wet/gray

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22
Q

B. pseudomallei SBA

A

“cream/tan
wrinkled”

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23
Q

E. meningoseptica SBA

A

yellow

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24
Q

P. aeruginosa SBA

A

“B/G
dry or mucoid
pigmented
scaled”

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25
Q

P. fluorescens SBA

A

“med
wet/gray”

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26
Q

P. stutzeri SBA

A

“dry/wrinkled
yellow”

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27
Q

S. putrifaciens SBA

A

“orange/tan
mucoid”

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28
Q

S. maltophila SBA

A

yellow

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29
Q

May oxidize lactose to give false + on MAC

A

A. baumanii
B. cepacia
B. pseudomallei

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30
Q

Ox + nonferm GNRs (5)

A

Burkholderia
Elizabethkingia meningoseptica
Moraxella osloensis
Pseudomonas
Shewenella putrifaciens

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31
Q

Ox variable

A

Burkholderia cepacia

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32
Q

Test unique to E. meningoseptica

A

Indole

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33
Q

Tests unique to S. putrifaciens

A

H2S
Ornithine

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34
Q

Test unique to S. maltophila

A

DNase

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35
Q

OF G/L/M
+/+/+
(2)

A

A. baumanii
B. pseudomallei

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36
Q

OF G/L/M
+/V/V
(1)

A

B. cepacia

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37
Q

OF G/L/M
+/=/+
(4)

A

E. meningoseptica
S. maltophila
P. aeruginosa
P. stutzeri

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38
Q

OF G/L/M
=/=/=
(2)

A

Moraxella
S. putrifaciens

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39
Q

OF G/L/M
+/=/=
(1)

A

P. fluorescens

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40
Q

Nitrate + (4)

A

Elizabethkingia meningoseptica
Moraxella osloensis
Pseudomonas aeruginosa
Pseudomonas stutzeri

MEN with MORE Nitrate eat PEARS with STU

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41
Q

Arginine + nonferms (4)

A

Burkholderia mallei
Burkholderia pseudomallei
Pseudomonas aeruginosa
Pseudomonas fluorescens

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42
Q

Ornithine + (1)

A

S. putrifaciens

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43
Q

aerobic oxidation of CHO pathway name

A

Entner-Duodoroff pathway

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44
Q

Clues that an organism may be a nonferm GNR (6)

A

1) no CHO ferm (TSI, KIA)
2) Ox +
3) Poor growth on MAC
4) Odors, colors, morphologies
5) Antibiotic resistance
6) No positive rxns in tests used for Enterobacteriaceae

45
Q

OF tubes ingredients

A

0.2% peptones
1% CHO
bromothymol blue

46
Q

most common 3 nonferm isolates

A

P. aeruginosa, A. baumannii, S. maltophilia

47
Q

motility test temperature for nonferms

48
Q

Brownian movement

A

natural movement of organisms on a wet mount

not motility

49
Q

2 additional pigments that nonferms can have

A

pyomelanin
pyorubin

50
Q

color of control tube for nonferm decarboxylase reactions

A

dirty gray

no glucose ferm, not yellow

51
Q

decarb tests for nonferm need to be incubated —– days to confirm a true =

52
Q

yellow, water-soluble pigment
function

A

pyoverdine
iron acquisition

53
Q

nonferm GNRs generally like what environments?

A

wet, humid

54
Q

pyocyanin function

A

toxin that kills mammalian and bacterial cells

55
Q

Paer virulence factors (10)

A

LPS
Hemolysins
Exotoxins
Cytotoxins
Proteolytic enzymes
Capsule and biofilm
Pili
Leukocidin
Pyocyanin
Intrinsically MDR

56
Q

Paer (does/does not) colonize healthy people

57
Q

risk factors for Paer colonization (6)

A

Burns
Cystic fibrosis (CF)
Ventilator use, tracheostomy
Organ Transplants
Leukemia
Neutropenia
IV drug addiction

58
Q

Paer clinical significance

A

Wound infections
Folliculitis
UTI
LRT infection (CF, COPD)
septicemia
keratitis
osteomyelitis
fingernail bed infections

59
Q

Problematic organisms in CF

A

Paer
Bcep
Smal

60
Q

polysaccharide reponsible for mucoid Paer

61
Q

rapid ID of Paer (5)

A

NLF GNR
Beta
Oxidase +
Fruity odor of grapes/warm corn tortillas
Metallic sheen and/or Blue-green pigment

62
Q

Paer is so MDR, —— is used to tx CF patients with it

A

high dose aerosolized tobramycin
oral azithromycin

63
Q

Pflu optimal growth temp

64
Q

Pflu associated with…

A

transfusion acquired septicemia
spoilage of refrigerated food

65
Q

Only difference between Pflu and P. putida

A

Pflu will grow at 4°

66
Q

Nonfluorescent Pseudomonas

A

P. stutzeri

67
Q

Bioterror agents

A

B. anthracis
B. pseudomallei
B. mallei

68
Q

B. cepacia complex includes —- species

69
Q

started as a plant pathogen

70
Q

Known for pseudo-infections associated with contaminated equipment, disinfectants and soaps

71
Q

Bcep main clinical significance

A

complication of CF

72
Q

Bcep in CF leads to death via…

A

lung failure

73
Q

3 selective agars for Bcep

A

BC agar
OFPBL agar
BCSA

74
Q

other disease that Bcep can complicate

leads to…

A

chronic granulomatous disease (CGD)

recurrent life-threatening bacterial and fungal infections

75
Q

must use 2 methods of ID

A

Bcep in CF pt

76
Q

Differentiate Bcep from Smal as they are both Lysine +

77
Q

Drug of choice for Bcep, which is extremely MDR

78
Q

used to degrade agent orange

79
Q

Found in southeast Asia, causes melioidosis

A

B. pseudomallei

80
Q

Must use antibiotics that penetrate WBC and treat for up to 6 months

Can hide and cause infection decades later

81
Q

Differentiates Bpse from Pstut as they both have wrinkled colonies

A

OF lactose
Arginine

82
Q

Commercial systems can mis-ID ——- as Pseudomonas

A

B. pseudomallei

83
Q

Causes glanders

84
Q

Primarily a respiratory disease of equines (horses, mules, donkeys)

A

Bmal (glanders)

85
Q

4 forms of glanders

A

Acute localized infection
Septicemic illness
Acute pulmonary infection
Chronic suppurative disease

86
Q

High fatality rate
90-95% without treatment
50% with antibiotic therapy

87
Q

Used as biowarfare agent in WW1

88
Q

May only be pathogenic when it acts in combination with other pathogens present

89
Q

Smal clinical significance

A

Pneumonia
Catheter related bacteremia
UTI
Wound infection
Sign that CF pt is deteriorating

90
Q

Septicemia with metastatic abscesses on skin, in lungs and other organs

Vietnamese time bomb

91
Q

Differentiates Smal from Enterobacteriaceae

92
Q

Drugs of choice for Smal

A

SXT
Colistin
Polymixin B

93
Q

Aquatic or marine environment but does not require NaCl for growth

A

Shewanella

94
Q

Strong gross fishlike odor

A

Shewanella

95
Q

Highly pathogenic for premature infants

A

E. meningoseptica

96
Q

Emen is sensitive to…

A

antibiotics regularly used for GPs (vanc, B-lactams, SXT, rifampin)

97
Q

Moraxella will appear more like rods after…

A

exposure to antibiotics

98
Q

M. lacunata clinical significance

A

conjunctivitis and keratitis

99
Q

Differentiates M. lacunata from Mcat

A

Mlac is penicillin susceptible

100
Q

A. baumanii mimics —- on GS

A

Neisseria, Mcat, Staph

101
Q

Differentiates A. baumanii from Neisseria

102
Q

Abau’s favorite CHO

103
Q

Leading cause of ventilator-associated pneumonia

104
Q

traumatic injuries in individuals after natural disasters such tornadoes and tsunamis

105
Q

Abau’s nicknames

A

great impersonator
Iraqibacter
Gram-negative MRSA

106
Q

mini biochemicals for nonferms

A

RapID NF Plus System

107
Q

RapID NF Plus System incubation

108
Q

Nonfermenting GNR genera (7)

A

Acinetobacter
Burkholderia
Elizabethkingia
Moraxella
Pseudomonas
Shewanella
Stenotrophomonas

Mercy CLS 2: Microbiology (16 decks)

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