Comprehensive—Organisms Flashcards
1
Q
Abiotrophia requirements
A
pyridoxal (vitamin B6)
2
Q
Abiotrophia rxns
A
SBA ng
Saur satellite +
E/P/L =/+/+
BE/salt =/=
3
Q
no valid method for susceptibility testing (5)
A
Abiotrophia
Aerococcus
Micrococcus luteus
P. fluorescens
S. saprophyticus
4
Q
“nutritionally variant strep”
A
Abiotrophia
5
Q
A. baumannii disease
A
nosocomial
immunocompromised hospitalized pts
after natural disasters
6
Q
A. baumannii morph
A
Gamma
smooth
gray
wet
7
Q
A. baumannii rxns
A
MAC oxidizer
oxidase =
OF G/L/M +/+/+
motility =
nitrate =
ALO =/=/=
8
Q
A. baumannii SP
A
MDR
9
Q
A. baumannii nicknames
A
great impersonator
GN MRSA
Iraqibacter
10
Q
A. israelii GS
A
GPR
filamentous
branching
11
Q
A. israelii rxns
A
catalase =
12
Q
Aerococcus Gs
A
GPCCL/tetrads
13
Q
Aerococcus morph
A
Alpha
gray
14
Q
Aerococcus rxns
A
catalse =/wk+
BE/salt V/+
15
Q
Aerococcus SP
A
susceptible to penicillin and vancomycin
no valid method for susceptibility testing
16
Q
A. hydrophila sites
A
water
marine animals
leeches
17
Q
A. hydrophila morph
A
Beta
medium
smooth
gray
18
Q
A. hydrophila rxns
A
oxidase +
glucose ferm +
indole +
Dnase +
gelatinase +
19
Q
A. actinomycetemcomitans morph
A
star shape after 5-6 days
20
Q
A. aphrophilus diseases
A
endocarditis
head/neck infections
those with oral piercings
21
Q
A. aphrophilus morph
A
faint yellow
round
smooth
grade school paste odor
22
Q
A. aphrophilus requirement
A
5-10% CO2
factor X on initial isolation
23
Q
A. aphrophilus rxns
A
SBA growth
Quad: X+ V+ XV+ HBA=
24
Q
A. haemolyticum rxns
A
catalase =
EPL =/=/=
motility =
25
B. anthracis diseases
cutaneous anthrax (eschar)
pulmonary anthrax ☠️
intestinal anthrax (rare)
26
B. anthracis morph
Gamma (diff. from Bacillus spp.)
white, ground glass
medusa
tenacious
27
B. anthracis rxns
catalase =
motility =
PEA ng
28
B. cereus disease
food poisoning
eye infection due to trauma
burn wounds
29
B. cereus morph
Beta
large
green, blue
feathered edges
30
B. cereus rxns
catalase +
motility +
31
cat scratch fever
Bartonella henselae
32
B. pertussis agars
Bordet-Gengou agar
Reagan-Lowe agar
33
Borrelia stain
Giemsa stain
34
Brucella diseases
undulant fever
systemic disease
35
Brucella rxns
catalse +
oxidase +
urease +
36
B. cepacia morph
yellow
dirt odor
37
B. cepacia rxns
MAC oxidizer
BC agar +
motility +
lysine +
38
B. cepacia SP
SXT drug of choice
39
B. mallei disease
glanders
40
B. mallei rxns
oxidase +
motility =
41
B. pseudomallei disease
melioidosis
42
B. pseudomallei morph
cream/tan
wrinkled
43
B. pseudomallei rxns
MAC oxidizer
OF GLM +/+/+
44
Vietnamese time bomb
B. pseudomallei
45
C. jejuni source
poultry
other farm animals
46
pre-Guillain Barre
C. jejuni
47
C. jejuni morph
wet; flat; tan/pink;
irregular
follow streak line
(campy bap)
48
C. jejuni requirements
5% O2
10% CO2
42°
49
C. jejuni rxns
catalase +
oxidase +
sodium hippurate +
urease =
50
C. canimorsus GS
GNR
fusiform; toothpick
51
C. canimorsus morph
yellow, tan, pink
moist
fingerlike projections
52
gliding motility
C. canimorsus
53
C. hominis GS
GVR
teardrop; dumbbell;
lollipop
picket fence
54
C. violaceum morph
black/purple
ammonium cyanide odor
55
C. freundii rxns
LF
VP =
PAD =
H2S +
Lysine =
ONPG +
56
C. koseri rxns
LLF
VP =
PAD =
H2S =
Motility +
Citrate +
Indole +
57
diphtheroid GS
GPR
club-shape
V/L formation
palisades
58
C. diphtheriae morph
Beta +/=
ppt/small
gray/white
59
C. diphtheria rxns
Cysteine-tellurite agar: black with brown halo (tellurite reductase)
catalase +
motility =
60
C. jeikium rxns
catalase +
glucose ferm +
motility =
urease =
61
Cjei + Cure SP
MDR
susceptible to vancomycin
62
C. urealyticum rxns
catalase +
glucose ferm =
motility =
urease +
63
C. sakazakii rxns
LF
VP =
PAD =
H2S =
Motility =
Citrate +
Indole =
64
E. corrodens morph
small, gray
lemon yellow
pits agar in CO2
bleach odor
65
E. meningosepitca morph
yellow
66
E. meningoseptica rxns
oxidase +
indole +
motility =
ALO =/=/=
67
E. meningoseptica SP
sensitive to GP antibiotics (vanc, SXT, BLs, etc)
68
E. aerogenes rxns
LF
VP +
Motility +
ALO =/+/+
DNase =
69
E. cloacae rxns
LF
VP +
Motility +
ALO +/=/+
70
E. faecalis SP
make up 10% VRE (vanB)
screen for HLAR
screen for B-lactamase
71
E. faecium SP
make up 90% VRE (vanA, vanB)
screen for HLAR
screen for B-lactamase
72
E. rhusiopathiae morph
Alpha weak
small
gray
73
E. rhusiopathiae rxns
catalase =
H2S + thin line (diff. from Saga)
74
E. rhusiopathiae SP
intrinsically resistant to vancomycin
75
E. coli morph
Beta
large
gray
wet
musty odor
76
E. coli rxns
dark dry LF
VP =
PAD =
H2S =
Motility +
Citrate =
SMAC nsf (O157:H7)
77
F. tularensis requirements
cysteine
78
H. ducreyi requirements
5-7% CO2
up to 10 days
factor X
79
H. ducreyi rxns
SBA ng
Quad: X+ V= XV+ HBA=
80
H. ducreyi grown on...
grown on Mueller-Hinton based CHOC with vancomycin
81
general Haem requirement
3-5% CO2
82
H. haemolyticus rxns
SBA ng
Quad: X= V= XV+ HBA+
ALA =
83
H. influenzae disease
meningitis (<6 yo)
sinusitis
otitis media
pneumonia
bronchitis
epiglottitis
conjunctivitis
periorbital cellulitis
systemic infection
Brazillian purpuric fever (H. aegyptius)
84
H. influenzae rxns
SBA ng
Quad: X= V= XV+ HBA=
ALA =
Saur satellite +
85
H. influenzae SP
B-lactamase +
chloramphenicol acetyltransferase +/=
86
H. parahaemolyticus rxns
SBA ng
Quad: X= V+ XV+ HBA+
ALA +
87
H. parainfluenzae rxns
SBA ng
Quad: X= V+ XV+ HBA=
ALA +
88
H. pylori rxns
CLO test + (urease on biopsy)
catalase +
oxidase +
89
H. pylori SP
requires triple drug therapy
90
K. kingae diseases
osteomyelitis in children
endocarditis
91
K. kingae morph
Beta soft
fried egg
pits agar
92
HACEK
Haemophilus, Aggregatibacter, Cardiobacterium, Eikenella, and Kingella
93
K. oxytoca rxns
LF
VP +
Motility =
Indole +
94
Klebsiella Sp
ESBL
carbapenemase
95
K. pneumoniae rxns
LF
VP +
Motility =
Indole =
96
L. pneumophila requirements
L-cysteine
iron salts
CO2
humidity
97
L. pneumophila detection
BCYE agar
DFA stain
urinary antigens
98
Leuconostoc diseases
infects those with invasive GI procedures
those on vancomycin
99
Leuconostoc rxns
catalase =
E/P/L V/=/=
BE/salt V/+
100
L. monocytogenes disease
neonates
cancer patients on chemo
meningoencephalitis
spontaneous abortion
101
L. monocytogenes morph
Beta soft
gray
small
102
L. monocytogenes rxns
catalase + (diff. from Saga)
motility 25° + (diff. from Saga)
BE/salt +/+ (diff. from Saga)
esculin +
sodium hippurate +
103
L. monocytogenes SP
falsely susceptible to cephalosporins
no resistance to date
104
M. luteus GS
GPC
tetrads, pairs, irregular clusters
105
M. luteus morph
pigmented (rose, orange, yellow, green)
106
M. luteus rxns
catalase +
Staph latex +
microdase + (diff. from Staph)
107
M. catarrhalis diseases
otitis media
bronchitis
pneumonia
acute maxillary sinusitis
108
M. catarrhalis morph
large
gray-white
hockey puck
109
M. catarrhalis rxns
oxidase +
CTA: G/M/L/S =/=/=/= (asaccharolytic)
butyrate esterate + (diff. from Neis)
DNase + (diff. from Neis)
TM growth
110
M. osloensis rxns
oxidase +
OF GLM =/=/=
ALO =/=/=
111
M. osloensis SP
B-lactamase =
112
M. morganii rxns
NLF
VP =
PAD +
H2S =
Citrate =
113
N. cinerea disease
proctitis
114
other Neisseria morph
yellow/xanthochromic
115
N. cinerea rxns
oxidase +
CTA: G/M/L/S =/=/=/= (asaccharolytic)
ng on TM after subculture
116
N. gonorrhoeae diseases
gonorrhoea
petechiae
septic arthritis
ophthalmia neonatorum
pelvic inflammatory disease
proctitis
pharyngitis
tonsillitis
117
Neisseria virulence factors
LOS
some capsule
118
Ngon morph
CHOC: medium, opaque, gray/tan, glistening, mucoid +/=, size variation
SBA: ppt or ng
119
Ngon SP
B-lactamase +
cephalosporin resistant
ceftriaxone susceptible (single dose)
120
N. lactamica rxns
oxidase +
CTA: G/M/L/S +/+/+/=
121
N. meningitidis diseases
meningitis
meningococcemia
waterhouse-friderichsen syndrome
3mo - 1 year
college students/military
122
bleb release
N. meningitidis
123
Nmen morph
SBA: Gamma, blue-gray
CHOC: gray, medium, moist, glistening
124
Nmen serotypes
A, B, C, X, Y and W135 serotypes
serotype B not immunogenic
125
N. sicca morph
yellow/xanthochromic
dry, wrinkled
adherent
126
P. agglomerans morph
yellow
127
P. agglomerans rxns
LLF
VP +
Motility +
ALO =/=/=
128
P. multocida rxns
catalase +
oxidase +
indole +
motility =
129
Pediococcus diseases
infects those on vancomycin
those with GI abnormalities/surgeries
abscesses
bacteremia
meningitis
130
Pediococcus rxns
catalase =
E/P/L +/=/+ (diff. from Ecoc)
BE/salt +/+ (diff. from Sgal)
131
Pediococcus SP
intrinsically resistant to vancomycin
132
P. shigelloides source
contaminated water
fish/reptiles
133
P. shigelloides rxns
NLF
oxidase + (diff. from other Enterics)
arginine + (diff. from Vibrio)
Dnase =
gelatinase =
134
P. mirabilis rxns
LF
VP =
PAD +
H2S +
Indole =
urease rapid +
135
P. vulgaris rxns
LF
VP =
PAD +
H2S +
Indole +
urease rapid +
136
P. rettgeri rxns
LF
VP =
PAD +
H2S =
Citrate +
Urease +
137
P. stuartii rxns
LF
VP =
PAD +
H2S =
Citrate +
Urease =
138
P. aeruginosa diseases
cystic fibrosis
burns
wound infections
swimmer’s ear
osteomyelitis in children, commonly heel
fingernail bed infections
139
Paer virulence factors
alginate polysaccharide capsule
pyocyanin
140
Paer morph
Beta
mucoid +/=
pigmented
scaled
grape odor
141
Paer rxns
42° growth +
4° growth =
oxidase +
motility +
142
P. fluorescens diseases
transfusion associated septicemia
143
P. fluorescens rxns
42° growth =
4° growth +
oxidase +
motility +
144
P. stutzeri morph
yellow
dry/wrinkled
145
P. stutzeri rxns
oxidase +
motility +
ALO =/=/=
146
R. mucilaginosa morph
Gamma
white
sticky
147
R. mucilaginosa rxns
catalase =/wk+ (diff. from Staph)
5% salt = (diff. from Staph)
148
S. enterica typhi rxns
NLF
VP =
PAD =
H2S + (mustache)
Lysine +
ONPG =
149
S. marcescens rxns
LLF
VP +
Motility +
ALO =/+/+
DNase, gelatinase, lipase +
150
S. marcescens SP
very MDR
151
S. putrifaciens source
seawater
152
S. putrifaciens morph
orange/tan
mucoid
fishlike odor
153
S. putrifaciens rxns
NLF
oxidase +
H2S + thin line
motility +
ornithine +
154
Shigella rxns
NLF
VP =
PAD =
H2S =
Motility =
ONPG =
155
Shigella serotypes
A: S. dysenteriae
B: S. flexneri
C: S. boydii
D: S. sonnei
156
SNA rxns
catalase +
coagulase =
Staph latex =
MSA growth/yellow +/=
157
Saur disease
folliculitis
boil, stye, faruncle, carbuncle
osteomyelitis
impetigo
TSS
wound infection
septicemia
endocarditis
scalded skin syndrome
food poisoning
158
Saur virulence factors
protein A
hyaluronidase, fibrinolysin
coagulase
DNase
lipase
capsule (MRSA, f= Staph latex)
superantigens
TSST
exfoliatins
enterotoxin
panton-valentine leukocidin (CA-MRSA)
staphylokinase (f= coagulase)
159
Saur rxns
catalase +
coagulase +
Staph latex +
MSA growth/yellow +/+
PYR =
Spectra agar + (MRSA)
160
Saur SP
B-lactamase +
test for oxacillin (cefoxitin) resistance—MRSA; mecA → PBP2a
test for vancomycin resistance—VRSA
test for inducible clindamycin resistance
161
S. intermedius morph
Beta (+/=) double zone
white
162
S. intermedius rxns
catalase +
tube coagulase +
PYR +
Spectra agar f+
163
S. lugdenensis diseases
aggressive, destructive skin/soft tissue disease
164
S. lugdenensis morph
Beta narrow
opaque white to yellow
variation of colonies
bleach odor
165
S. lugdenensis rxns
catalase +
slide coagulase +
PYR +
166
S. lugdenensis SP
pan-sensitive
usually B-lactamase =
167
S. saprophyticus rxns
catalase +
coagulase =
Staph latex wk+
novobiocin R (<12mm; diff. from other Staph)
168
S. maltophilia morph
yellow
ammonia odor
169
S. maltophilia rxns
NLF
oxidase =
DNase +
OF GLM +/=/+
motility +
lysine +
170
S. maltophilia SP
intrinsically MDR
SXT drug of choice
171
S. moniliformis GS
GNR
pleiomorphic
string of beads
172
S. agalactiae rxns
catalase =
PYR =
carrot broth +
sodium hippurate +
173
S. agalactiae SP
penicillin susceptible
gentamicin susceptible
174
isolation from blood → colon cancer
S. gallolyticus
175
S. gallolyticus morph
Gamma/slight Alpha
gray
176
S. gallolyticus rxns
catalase =
E/P/L +/=/+
BE/salt +/= (diff. from Ecoc)
177
S. gallolyticus SP
pan-susceptible
178
S. pneumoniae diseases
pneumonia
bacteremia
endocarditis
meningitis
sinusitis
otitis media
conjunctivitis
179
S. pneumoniae morph
Alpha
regular (umbilicated) or mucoid (presumptive type III)
180
S. pneumoniae requirements
CO2 (autolyses without)
liquid media triggers autolysis
181
S. pneumoniae rxns
catalase =
optochin S (>14 mm)
bile solubility +
urine antigen + (pneumonia, meningitis)
182
S. pneumoniae SP
increasing penicillin resistance
vancomycin susceptible
screen for inducible clindamycin resistance
183
S. pyogenes diseases
pharyngitis
tonsillitis
impetigo
pyoderma
scarlet fever
TSS
cellulitis
necrotizing fasciitis
acute rheumatic fever
acute glomerulonephritis
PANDAS
184
S. pyogenes virulence factors
SLO
SLS
streptokinase
streptodornase (DNase)
pyrogenic exotoxin (scarlet fever)
M protein
hyaluronidase
185
S. pyogenes morph
Beta wide
gray
small, domed
186
S. pyogenes rxns
catalase =
E/P/L =/+/+ (only B-Strep that is PYR +)
bacitracin S (A-disk)
187
Strep CGF diseases
C—pharyngitis, soft tissue infection
G—pharyngitis
F—purulent head abscesses; endocarditis; osteomyelitis
188
Strep CGF morph
Beta wide
ppt
gray
butterscotch/honeysuckle/caramel odor
189
Strep CGF rxns
catalase =
PYR =
190
viridans diseases
subacute bacterial endocarditis (from oral site)
tooth decay (mutans)
gingivitis (mutans)
191
viridans morph
Alpha/Gamma
gray
ppt (anginosus)
192
viridans rxns
catalase =
E/P/L =/=/+
BE/salt =/=
193
V. cholerae rxns
TCBS yellow
oxidase +
glucose ferm +
indole +
arginine =
DNase +
gelatinase +
194
Y. enterocolitica diseases
mesenteric lymphadenitits, mimics appendicitis
septicemia in those with iron overload
195
Yersinia rxns
NLF
VP =
PAD =
H2S =
Motility = (35°)
ONPG +
196
bipolar/safety pin
Y. pestis
197
definition of Enterobacteriaceae
GNR
Oxidase =
Glucose-fermenting
Grow on MacConkey
Reduce nitrates to nitrites
