9. Misc Organisms Flashcards
Aeromonas hydrophila
GS
GNR
Aeromonas hydrophila
SBA
“B
med; smooth; gray”
Aeromonas hydrophila
reservoir
“water
marine animals
leeches”
Aeromonas hydrophila
transmission
“seafood
water”
Aeromonas hydrophila
disease
“wound infection
septicemia
acute diarrheal disease”
oxidase =
Francisella tularensis
Aeromonas hydrophila
MAC
V
Aeromonas hydrophila
TSI/KIA
A/A G
V
indole =
Brucella
Eikenella
Aeromonas hydrophila
arginine
+
Aeromonas hydrophila
DNase/gelatinase
+
Aeromonas hydrophila mimics…
E. coli
Aggregatibacter actinomycetemcomitans
GS
“GNCB
pale”
Aggregatibacter actinomycetemcomitans
SBA
“adherent
stars after 5-6 days”
Aggregatibacter actinomycetemcomitans
reservoir
“oral flora
gingival crevices”
Aggregatibacter actinomycetemcomitans
disease
“endocarditis
juvenile destructive periodontitis”
Associated with A. israelii
Aggregatibacter actinomycetemcomitans
Aggregatibacter aphrophilus
GS
“GNCB
filamentous”
Aggregatibacter aphrophilus
requirements
“5-10% CO2
factor X on initial isolation”
Aggregatibacter aphrophilus
reservoir
oral flora
Aggregatibacter aphrophilus
disease
endocarditis
Bartonella henselae
GS
GNR
Bartonella henselae
reservoir
“cat flea
cats & kittens”
Bartonella henselae
transmission
insect bite
Bartonella henselae
disease
cat scratch fever
Bartonella henselae
specific tests/agars
serological tests
motility =
Pasteurella multocida
Bordatella bronchiseptica
GS
“GNCB/R
small; pale”
Bordatella bronchiseptica
disease
kennel cough (dogs)
Bordatella pertussis
GS
“GNCB
small; pale”
Bordatella pertussis
requirements
“charcoal
15-25% blood”
Bordatella pertussis
virulence factors
toxin
Bordatella pertussis
reservoir
unvaccinated humans
Bordatella pertussis
transmission
aerosol
Bordatella pertussis
specific tests/agars
“Bordet-Gengou agar
Reagan-Lowe agar”
biochemically inert
Bordatella pertussis
Borrelia burgdorferi
GS
“narrow spirochete
very long”
Borrelia burgdorferi
SBA
culture takes weeks
Borrelia burgdorferi
reservoir
deer tick (nymph)
Borrelia burgdorferi
transmission
insect bite
Borrelia burgdorferi
disease
Lyme
Borrelia burgdorferi
treatment
doxycycline (w/i 72 hr)
Borrelia burgdorferi
specific tests/agars
serological tests
Borrelia stain
Giemsa stain
Borrelia recurrentis
GS
thick spirochete
Borrelia recurrentis
virulence factors
“extremely fast surface antigen variation (q 7 days)”
Borrelia recurrentis
reservoir
body louse (humans)
Borrelia recurrentis
transmission
insect bite
Borrelia recurrentis
disease
relapsing fever
Borrelia recurrentis
treatment
“penicillin; tetracycline;
erythromycin”
Brucella
GS
“GNCB
tiny, faint”
Brucella
SBA
“small; gray; smooth;
wet”
Brucella abortus
reservoir
cattle; bison; camels
Brucella
transmission
“travel to endemic area
animal contact
animal food products
aerosol (lab acquired)”
Brucella
disease
“systemic disease
undulant fever”
Brucella
specific tests/agars
Cateñeda bottle
catalase =
Eikenella
Francisella
Urease +
Brucella
H. pylori
Bioterror agents
Brucella
Francisella
Least virulent Brucella
Most virulent Brucella
Least - B. abortus
Most - B. melitensis
Brucella melitensis
reservoir
goats; sheep; camels
Brucella suis
reservoir
pigs
Campylobacter jejuni
GS
“GNR
small; squiggle”
Campylobacter jejuni
Campy BAP
“wet; flat; tan/pink;
irregular
follow streak line”
Campylobacter jejuni
requirements
5% O2
10% CO2
42°/4°
Campylobacter jejuni
virulence factors
“enterotoxin
cytotoxin”
Campylobacter jejuni
reservoir
“poultry
other farm animals”
Campylobacter jejuni
transmission
“F-O
water; milk; raw food
animal contact”
Campylobacter jejuni
disease
“gastroenteritis
pre-Guillain Barre”
Campylobacter jejuni
resistance
TMP/SMX
Campylobacter jejuni
specific tests/agars
Skirrow’s medium
Campy BAP/CVA
Hippurate +
Campylobacter jejuni
Capnocytophagia canimorsus
GS
“GNR
fusiform; toothpick”
Capnocytophagia canimorsus
SBA
“yellow, tan, pink
moist
fingerlike projections”
Capnocytophagia canimorsus
reservoir
oral (humans; dogs)
Capnocytophagia canimorsus
transmission
dog contact/bites
Capnocytophagia canimorsus
disease
“fatal sepsis
endocarditis”
gliding motility
Capnocytophagia canimorsus
Cardiobacterium hominis
GS
“GVR
teardrop; dumbbell;
lollipop
picket fence”
Cardiobacterium hominis
SBA
“ng for 48-72 hr
ppt”
Cardiobacterium hominis
reservoir
URT flora
Cardiobacterium hominis
disease
endocarditis
Chromobacterium violaceum
GS
GNR/CB
Chromobacterium violaceum
SBA
“black/purple
ammonium cyanide odor”
Chromobacterium violaceum
virulence factors
violacein pigment
Chromobacterium violaceum
reservoir
“environment
Southeast”
Chromobacterium violaceum
disease
“wound infection
(fatal in immunocomp. hosts)”
Glucose fermenters (5)
(those specifically named as such in ppt)
Aeromonas
Chromobacterium
Pasteurella
Plesiomonas
Vibrio
Eikenella corrodens
SBA
“small, gray
lemon yellow
pits agar in CO2
bleach odor”
Eikenella corrodens
requirements
hemin
Eikenella corrodens
disease
“human bite infections
dental infections
head/neck infections
aspiration pneumonia
bacteremia/endocarditis
nailbiting infections”
Eikenella corrodens
treatment
“penicillin; quinolones;
carbapenems”
Eikenella corrodens mimics…
Haemophilus
Eikenella corrodens
GS
“GNR/CB
slender”
Francisella tularensis
GS
“GNCB
tiny; pale”
Francisella tularensis
SBA
ng
Francisella tularensis
requirements
cysteine
Francisella tularensis
reservoir
rabbits, cats, rodents
Francisella tularensis
transmission
“direct contact with blood,
carcass, skin of animal
contaminated food
aerosol
insect bite
infected cat contact”
Francisella tularensis
disease
“tularemia/rabbit fever
(resembles plague)”
Francisella tularensis
specific tests/agars
“blood cysteine glucose agar
BCYE agar
Thayer-Martin agar”
Helicobacter pylori
GS
“GNR
spiral/helical”
Helicobacter pylori
SBA
small; gray; translucent
Helicobacter pylori
requirements
“5% O2
10% CO2
humidity”
Helicobacter pylori
reservoir
stomach epithelia
Helicobacter pylori
transmission
“unknown
ingestion”
Helicobacter pylori
disease
“gastritis
ulcers
cancer”
Helicobacter pylori
treatment
triple drug therapy
Helicobacter pylori
specific tests/agars
“CLO test (biopsy)
13CO2 breath”
Kingella kingae
GS
“GNCB
plump
chains/pairs
may stain GP”
Kingella kingae
SBA
“B soft
fried egg
pits agar”
Kingella kingae
reservoir
URT & GU flora
Kingella kingae
disease
“bacteremia; endocarditis
septic arthritis
osteomyelitis
(children)”
Legionella pneumophila
GS
“GNR/CB
thin, pleiomorphic”
Legionella pneumophila
requirements
“L-cysteine
iron salts
CO2
humidity”
Legionella pneumophila
reservoir
“water
manmade env.
warmth”
Legionella pneumophila
transmission
“aerosols
no person-person trans.”
Legionella pneumophila
disease
“Legionnaires disease
Pontiac fever”
Legionella pneumophila
specific tests/agars
“BCYE agar
DFA stain
urinary antigens”
Pasteurella multocida
GS
“GNCB/R
pleiomorphic”
Pasteurella multocida
SBA
“smooth; wet; gray
musty/mushroom odor”
Pasteurella multocida
requirements
“CO2
hemin”
Pasteurella multocida
virulence factors
antiphagocytic capsule
Pasteurella multocida
reservoir
“cats, dogs, other
(oral)
human URT”
Pasteurella multocida
transmission
“cat scratch/bite
dog bite”
Pasteurella multocida
disease
“osteomyelitis
tenosynovitis
septic arthritis
pneumonia”
Plesiomonas shigelloides
GS
GNR
Plesiomonas shigelloides
SBA
large; wet; gray
Plesiomonas shigelloides
reservoir
freshwater
Plesiomonas shigelloides
transmission
“contaminated water
fish/reptiles”
Plesiomonas shigelloides
disease
“gastroenteritis
wound infections
septicemia”
Plesiomonas shigelloides
treatment
“quinolones; TMP/SMX;
doxycycline; aztreonam”
Plesiomonas shigelloides
MAC
NLF
Plesiomonas shigelloides
TSI/KIA
V
K/A
Plesiomonas shigelloides
arginine
+
Plesiomonas shigelloides
DNase/gelatinase
=
Plesiomonas shigelloides mimics…
E. coli
Streptobacillus moniliformis
GS
“GNR
pleiomorphic
string of beads”
Streptobacillus moniliformis
requirements
“10-20% blood,
serum, ascites fluid”
Streptobacillus moniliformis
reservoir
rodents (URT)
Streptobacillus moniliformis
transmission
rodents
Streptobacillus moniliformis
disease
“rat bite fever
fatal complications”
Treponema pallidum
GS
“GNR
helically coiled”
Treponema pallidum
SBA
nonculturable
Treponema pallidum
reservoir
humans
Treponema pallidum
transmission
“sex; direct contact;
needles; transplacental”
Treponema pallidum
disease
“syphilis
skin infections (tropics)”
Treponema pallidum
treatment
penicillin G; doxycycline
Treponema pallidum
specific tests/agars
“serologic testing
dark-field microscopy”
Vibrio
GS
“GNR
comma”
Vibrio
reservoir
“seawater
seafood”
Vibrio
arginine
=
Vibrio cholerae
SBA
wet; gray; green tint
Vibrio cholerae
requirements
no salt req.
Vibrio cholerae
virulence factors
cholera toxin (O1 type)
Vibrio cholerae
transmission
undercooked seafood
contaminated water
Vibrio cholerae
treatment
fluid/EL replacement
Vibrio cholerae
specific tests/agars
TCBS agar
yellow
Vibrio cholerae
MAC
NLF
Vibrio cholerae
TSI/KIA
A/A
K/A
Vibrio cholerae
DNAse/gelatinase
+
Vibrio parahaemolyticus
SBA
wet; gray
Vibrio parahaemolyticus
requirements
1% NaCl
Vibrio parahaemolyticus
transmission
“seafood in summer
oysters
seawater”
Vibrio parahaemolyticus
disease
“diarrhea
wound infection”
Vibrio parahaemolyticus
MAC
NLF
Vibrio parahaemolyticus
TSI/KIA
K/A
Vibrio vulnificus
requirements
1% NaCl
Vibrio vulnificus
transmission
“seawater
raw seafood”
Vibrio vulnificus
disease
“gastroenteritis
fatal wound infection
necrotizing fasciitis”
Vibrio vulnificus
MAC
V
Vibrio vulnificus
TSI/KIA
V
K/A
Vibrio vulnificus
DNAse/gelatinase
+
require hemin
E. corrodens
P. multocida
require cysteine
F. tularensis
L. pneumophila
arginine +
A. hydrophila
P. shigelloides
arginine =
Vibrio
DNAse/gelatinase =
P. shigelloides
DNAse/gelatinase +
A. hydrophila
Vibrio
