7. GPR Flashcards
1
Q
Actinomyces israelii GS
A
“GPR
filamentous
branching”
2
Q
Actinomyces israelii SBA
A
molar tooth
3
Q
Arcanobacterium haemolyticum GS
A
“GPR
curved
branching”
4
Q
Arcanobacterium haemolyticum SBA
A
“B subtle
small
gray/white”
5
Q
Arcanobacterium haemolyticus E/P/L
A
=/=/=
6
Q
Bacillus GS
A
“GP/VR
spores
large, boxy”
7
Q
Bacillus anthracis SBA
A
“G
white, ground glass
tenacious”
8
Q
Bacillus cereus SBA
A
“B
large
green
feathered edges”
9
Q
Corynebacterium GS
A
“GPR
club-shape
V/L formation
palisades”
10
Q
Corynebacterium diphtheriae SBA
A
“B +/=
ppt/small
gray/white”
11
Q
Corynebacterium jeikeium/urealyticum SBA
A
“G
ppt
gray/white”
12
Q
Erysipelothrix rhusiopathiae GS
A
“GPR
filamentous”
13
Q
Erysipelothrix rhusiopathiae SBA
A
“A weak
small
gray”
14
Q
Gardnerella vaginalis GS
A
GVR
15
Q
Lactobacillus GS
A
“GPR
very long”
16
Q
Lactobacillus SBA
A
“A
small/ppt
gray”
17
Q
Listeria monocytogenes GS
A
GPR/CB
18
Q
Listeria monocytogenes SBA
A
“B subtle
small
gray”
19
Q
Spyo SBA
A
B wide zone
20
Q
Saga SBA
A
“B subtle
small
gray”
21
Q
C. diphtheriae appears black with a brown halo on —– agar
A
Cystine-Tellurite/Tinsdale
22
Q
Catalase = GPRs (5)
A
Actinomyces israelii
Arcanobacterium haemolyticum
Erysipelothrix rhusiopathiae
Gardnerella vaginalis
Lactobacillus
23
Q
Catalase + GPRs (3)
A
Bacillus
Corynebacterium
Listeria monocytogenes
Cats CRYing with MONO love BACk scratches
24
Q
Erhu BE/salt
A
=/+
25
Lmon BE/salt
+/+
26
Saga BE/salt
=/+
27
Lmon esculin
+
28
Spyo E/P/L
=/+/+
29
Cjei vs Cure
Cjei Gluose +/Urea =
Cure Glucose =/Urea +
30
Positive hippurates
Lmon
Saga
31
Bant vs Bcer (3)
Bant Motility =; S to penicillin; does not grow on PEA
32
Lmon motility
+
tumbling
umbrella SIM
33
H2S + GPR
Erhu
34
Corynebacterium diphtheriae
35
Corynebacterium NF site
URT
skin
36
Cdip transmission
respiratory aerosol
37
Coryne energy storage
polyphosphate granules
38
Enzyme responsible for Coryne's black colony on Tinsdale agar
tellurite reductase
39
Other organism that can grow black on Tinsdale
Saur
40
used to determine if Coryne is making toxins
principle of test
Elek test
immunoprecipitation
41
Elek test
42
Cdip on SBA can resemble...
What differentiates? (4)
Spyo
GS; catalase; dark halo around Cdip on Tinsdale; Loeffler's slant for granule formation
43
Non-selective medium used to enhance the formation of metachromatic granules
Loeffler’s serum slant
44
What is Colistin-nalidixic acid (CNA) agar used for?
Cdip
distinguish Cdip from others of the genus; isolate it out of normal throat flora
45
CNA selects for -----, like PEA
GPs
46
Required for Coryne to make toxin
Organism must first be attacked by a bacteriophage that encodes it for toxin production
47
Cdip toxin effects
Causes arrhythmia, myocarditis, congestive heart failure, cranial and peripheral palsy, paralysis of diaphragm
48
Diphtheria presents as....
pharyngitis/tonsilitis
49
Reservoir for Cdip
adults who have not kept up with vaccinations
50
initially mimics white spots on tonsils found in Strep
Cdip pseudomembrane
51
bull neck
diphtheria
52
non-respiratory infection caused by diphtheria
skin lesions
53
Gray membrane over skin lesion that peels off to reveal a beefy wound
Cdip
54
disease does not confer immunity
Cdip
55
non-diphtheriae Coryne can look like...
SNA
56
Cjei clinical significance (8)
nosocomial infections
colonization of hospitalized patients (Inguinal, Axillary, Rectal, Perineum)
Endocarditis/septicemia
CSF shunt/catheter
Pneumonia
Osteomyelitis
Peritonitis
Surgical wound infection
57
Antibiotics used to ID Cjei/Cure
MDR, except susceptible to Vanc
58
Rapidly urease +
Cure
59
Cure clinical significance
Acute and chronic urinary tract infections
Renal cysts
Renal stones
Alkaline-encrusted cystitis (AEC)
60
Alkaline-encrusted cystitis (AEC)
Cure
61
Diphtheria tx
Antitoxin and penicillin or erythromycin
62
Foods implicated with Lmon
Coleslaw, soft cheeses (feta, brie, camembert, blue-veined), raw milk, undercooked chicken, hotdogs, sliced meats, fish and shellfish
63
Intracellular pathogen
Lmon
64
Lmon resembles...
Saga
65
Temperature that Lmon will grow at, and Saga will not
4°
66
can cross-react with Group B antiserum on strep latex test
Lmon
Ahae
67
Lmon clinical significance
meningoencephalitis
sepsis
spontaneous abortions
gastroenteritis
68
Typical adult patient of Lmon
cancer patient on chemotherapy or steroids
69
Lmon specimens
CSF
blood
stool
placenta
70
appears falsely susceptible to cephalosporins, which don't cross the blood brain barrier
Lmon
71
spores indicate Bacillus or....
clostridium
72
Bacillus
73
Bacillus O2
Facultative anaerobe
74
Bacillus PEA
growth
B. anthracis - no growth
75
Bacillus beneficial applications
Antibiotic production
Vitamin production
Validation of sterilization methods
Insect control
76
Bant primarily causes disease in...
ruminants
77
protein antiphagocytic capsule
Bant
78
2 toxins in Bant
edema toxin and lethal toxin
79
Bant transmission
through animal products
80
Most Bant infections are cases of ---------- and present as .......
cutaneous anthrax
Painless, slow healing black ulcer called an eschar
81
Toxin effects in resp. anthrax
Causes edema, internal bleeding, tissue damage and necrosis
82
Antibiotics do not work on resp. anthrax once...
toxin reaches bloodstream
83
Rare 3rd form of anthrax
intestinal anthrax
84
2001 bioterror organism
Bant
85
Bant safety procedures
class II biological safety cabinet
Need a class III cabinet for manipulations that may create aerosols (mincing , vortexing, cutting)
86
Bant vaccine recommended for...
Military, lab workers with direct contact with organism, and those who work with animals and their products imported from endemic areas
87
Do not use --------- to tx Bant because...
No penicillin due to possible inducible β-lactamase
88
most common lab contaminant
Bcer
89
2 forms of Bcer food poisoning due to toxin
Emetic
Diarrheal
90
Most often associated with consuming fried rice at Asian restaurants
Bcer food poisoning
91
Can cause eye infection due to trauma, especially from farm accidents
Bcer
92
Antibiotic that separates Bcer from Bant
Bant is susceptible to penicillin
Bcer is resistant
93
Found in animals, pigs being the main reservoir
Erhu
94
Localized form of cellulitis caused by Erhu
erysipeloid
95
Lesions are purplish, swollen, painful and spread slowly
40% mortality if it goes septicemic
Erhu
96
Erhu specimen
Need to culture a full thickness biopsy of infected skin
97
intrinsically resistant to Vanc, teicoplanin, SXT, and aminoglycosides
Erhu
98
Lactobacillus importance
Important in maintaining pH of healthy female genital tract
99
intrinsically resistant to Vanc, infection is very rare
Lactobacillus
100
inhibited by SBS anticoag
G. vaginalis
101
gold standard for dx of BV
GS
102
shift that happens in BV
from good GP organisms to bad GN organisms
103
GN organisms that cause BV when Lactobacillus disappears
Gardnerella vaginalis
Bacteroides spp.
Prevotella spp.
Mobiluncus spp.
104
BV clinical sx
gray discharge, fishy odor with 10% KOH, pH >4.6
105
BD Affirm VPIII detection threshold is set.....
above levels of normal flora and detects only clinically significant levels (disease state) of Gardnerella and Candida species
106
BD Affirm VPIII is useful for...
fast results (1 hour) for pregnant women at delivery
107
Complications of BV in pregnant women
Preterm birth
Premature rupture of the membranes (PROM)
Chorioamnionitis
108
Shows similar arrangement on GS to diphtheroids
Arcanobacterium haemolyticum
109
A. haemolyticum mimics...
S. pyogenes or S. anginosus
110
Distinguishes Ahae from Spyo (3)
GS; LAP = (Spyo +); PYR = (Spyo +)
111
Causes pharyngitis that can be mistaken for Strep
A. haemolyticum
112
A. israelii
113
A. israelii clinical significance
cervicofacial actinomycosis
114
colonies of organisms in pus resemble hard, yellow sulfur granules
A. israelii
115
risk factors for actinomycosis
dental procedures
oral surgery
IUD
116
GPR genera (8)
Actinomyces
Arcanobacterium
Bacillus
Corynebacterium
Erysipelothrix
Gardnerella
Lactobacillus
Listeria
