7. GPR Flashcards
Actinomyces israelii GS
“GPR
filamentous
branching”
Actinomyces israelii SBA
molar tooth
Arcanobacterium haemolyticum GS
“GPR
curved
branching”
Arcanobacterium haemolyticum SBA
“B subtle
small
gray/white”
Arcanobacterium haemolyticus E/P/L
=/=/=
Bacillus GS
“GP/VR
spores
large, boxy”
Bacillus anthracis SBA
“G
white, ground glass
tenacious”
Bacillus cereus SBA
“B
large
green
feathered edges”
Corynebacterium GS
“GPR
club-shape
V/L formation
palisades”
Corynebacterium diphtheriae SBA
“B +/=
ppt/small
gray/white”
Corynebacterium jeikeium/urealyticum SBA
“G
ppt
gray/white”
Erysipelothrix rhusiopathiae GS
“GPR
filamentous”
Erysipelothrix rhusiopathiae SBA
“A weak
small
gray”
Gardnerella vaginalis GS
GVR
Lactobacillus GS
“GPR
very long”
Lactobacillus SBA
“A
small/ppt
gray”
Listeria monocytogenes GS
GPR/CB
Listeria monocytogenes SBA
“B subtle
small
gray”
Spyo SBA
B wide zone
Saga SBA
“B subtle
small
gray”
C. diphtheriae appears black with a brown halo on —– agar
Cystine-Tellurite/Tinsdale
Catalase = GPRs (5)
Actinomyces israelii
Arcanobacterium haemolyticum
Erysipelothrix rhusiopathiae
Gardnerella vaginalis
Lactobacillus
Catalase + GPRs (3)
Bacillus
Corynebacterium
Listeria monocytogenes
Cats CRYing with MONO love BACk scratches
Erhu BE/salt
=/+
Lmon BE/salt
+/+
Saga BE/salt
=/+
Lmon esculin
+
Spyo E/P/L
=/+/+
Cjei vs Cure
Cjei Gluose +/Urea =
Cure Glucose =/Urea +
Positive hippurates
Lmon
Saga
Bant vs Bcer (3)
Bant Motility =; S to penicillin; does not grow on PEA
Lmon motility
+
tumbling
umbrella SIM
H2S + GPR
Erhu
Corynebacterium diphtheriae
Corynebacterium NF site
URT
skin
Cdip transmission
respiratory aerosol
Coryne energy storage
polyphosphate granules
Enzyme responsible for Coryne’s black colony on Tinsdale agar
tellurite reductase
Other organism that can grow black on Tinsdale
Saur
used to determine if Coryne is making toxins
principle of test
Elek test
immunoprecipitation
Elek test
Cdip on SBA can resemble…
What differentiates? (4)
Spyo
GS; catalase; dark halo around Cdip on Tinsdale; Loeffler’s slant for granule formation
Non-selective medium used to enhance the formation of metachromatic granules
Loeffler’s serum slant
What is Colistin-nalidixic acid (CNA) agar used for?
Cdip
distinguish Cdip from others of the genus; isolate it out of normal throat flora
CNA selects for —–, like PEA
GPs
Required for Coryne to make toxin
Organism must first be attacked by a bacteriophage that encodes it for toxin production