7. GPR Flashcards

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1
Q

Actinomyces israelii GS

A

“GPR
filamentous
branching”

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2
Q

Actinomyces israelii SBA

A

molar tooth

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3
Q

Arcanobacterium haemolyticum GS

A

“GPR
curved
branching”

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4
Q

Arcanobacterium haemolyticum SBA

A

“B subtle
small
gray/white”

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5
Q

Arcanobacterium haemolyticus E/P/L

A

=/=/=

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6
Q

Bacillus GS

A

“GP/VR
spores
large, boxy”

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7
Q

Bacillus anthracis SBA

A

“G
white, ground glass
tenacious”

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8
Q

Bacillus cereus SBA

A

“B
large
green
feathered edges”

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9
Q

Corynebacterium GS

A

“GPR
club-shape
V/L formation
palisades”

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10
Q

Corynebacterium diphtheriae SBA

A

“B +/=
ppt/small
gray/white”

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11
Q

Corynebacterium jeikeium/urealyticum SBA

A

“G
ppt
gray/white”

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12
Q

Erysipelothrix rhusiopathiae GS

A

“GPR
filamentous”

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13
Q

Erysipelothrix rhusiopathiae SBA

A

“A weak
small
gray”

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14
Q

Gardnerella vaginalis GS

A

GVR

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15
Q

Lactobacillus GS

A

“GPR
very long”

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16
Q

Lactobacillus SBA

A

“A
small/ppt
gray”

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17
Q

Listeria monocytogenes GS

A

GPR/CB

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18
Q

Listeria monocytogenes SBA

A

“B subtle
small
gray”

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19
Q

Spyo SBA

A

B wide zone

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20
Q

Saga SBA

A

“B subtle
small
gray”

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21
Q

C. diphtheriae appears black with a brown halo on —– agar

A

Cystine-Tellurite/Tinsdale

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22
Q

Catalase = GPRs (5)

A

Actinomyces israelii
Arcanobacterium haemolyticum
Erysipelothrix rhusiopathiae
Gardnerella vaginalis
Lactobacillus

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23
Q

Catalase + GPRs (3)

A

Bacillus
Corynebacterium
Listeria monocytogenes

Cats CRYing with MONO love BACk scratches

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24
Q

Erhu BE/salt

A

=/+

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25
Q

Lmon BE/salt

A

+/+

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26
Q

Saga BE/salt

A

=/+

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27
Q

Lmon esculin

A

+

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28
Q

Spyo E/P/L

A

=/+/+

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29
Q

Cjei vs Cure

A

Cjei Gluose +/Urea =

Cure Glucose =/Urea +

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30
Q

Positive hippurates

A

Lmon
Saga

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31
Q

Bant vs Bcer (3)

A

Bant Motility =; S to penicillin; does not grow on PEA

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32
Q

Lmon motility

A

+
tumbling
umbrella SIM

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33
Q

H2S + GPR

A

Erhu

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34
Q
A

Corynebacterium diphtheriae

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35
Q

Corynebacterium NF site

A

URT
skin

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36
Q

Cdip transmission

A

respiratory aerosol

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37
Q

Coryne energy storage

A

polyphosphate granules

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38
Q

Enzyme responsible for Coryne’s black colony on Tinsdale agar

A

tellurite reductase

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39
Q

Other organism that can grow black on Tinsdale

A

Saur

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40
Q

used to determine if Coryne is making toxins

principle of test

A

Elek test

immunoprecipitation

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41
Q
A

Elek test

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42
Q

Cdip on SBA can resemble…

What differentiates? (4)

A

Spyo

GS; catalase; dark halo around Cdip on Tinsdale; Loeffler’s slant for granule formation

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43
Q

Non-selective medium used to enhance the formation of metachromatic granules

A

Loeffler’s serum slant

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44
Q

What is Colistin-nalidixic acid (CNA) agar used for?

A

Cdip

distinguish Cdip from others of the genus; isolate it out of normal throat flora

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45
Q

CNA selects for —–, like PEA

A

GPs

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46
Q

Required for Coryne to make toxin

A

Organism must first be attacked by a bacteriophage that encodes it for toxin production

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47
Q

Cdip toxin effects

A

Causes arrhythmia, myocarditis, congestive heart failure, cranial and peripheral palsy, paralysis of diaphragm

48
Q

Diphtheria presents as….

A

pharyngitis/tonsilitis

49
Q

Reservoir for Cdip

A

adults who have not kept up with vaccinations

50
Q

initially mimics white spots on tonsils found in Strep

A

Cdip pseudomembrane

51
Q

bull neck

A

diphtheria

52
Q

non-respiratory infection caused by diphtheria

A

skin lesions

53
Q

Gray membrane over skin lesion that peels off to reveal a beefy wound

A

Cdip

54
Q

disease does not confer immunity

A

Cdip

55
Q

non-diphtheriae Coryne can look like…

A

SNA

56
Q

Cjei clinical significance (8)

A

nosocomial infections

colonization of hospitalized patients (Inguinal, Axillary, Rectal, Perineum)

Endocarditis/septicemia
CSF shunt/catheter
Pneumonia
Osteomyelitis
Peritonitis
Surgical wound infection

57
Q

Antibiotics used to ID Cjei/Cure

A

MDR, except susceptible to Vanc

58
Q

Rapidly urease +

A

Cure

59
Q

Cure clinical significance

A

Acute and chronic urinary tract infections
Renal cysts
Renal stones
Alkaline-encrusted cystitis (AEC)

60
Q

Alkaline-encrusted cystitis (AEC)

A

Cure

61
Q

Diphtheria tx

A

Antitoxin and penicillin or erythromycin

62
Q

Foods implicated with Lmon

A

Coleslaw, soft cheeses (feta, brie, camembert, blue-veined), raw milk, undercooked chicken, hotdogs, sliced meats, fish and shellfish

63
Q

Intracellular pathogen

A

Lmon

64
Q

Lmon resembles…

A

Saga

65
Q

Temperature that Lmon will grow at, and Saga will not

A

66
Q

can cross-react with Group B antiserum on strep latex test

A

Lmon
Ahae

67
Q

Lmon clinical significance

A

meningoencephalitis
sepsis
spontaneous abortions
gastroenteritis

68
Q

Typical adult patient of Lmon

A

cancer patient on chemotherapy or steroids

69
Q

Lmon specimens

A

CSF
blood
stool
placenta

70
Q

appears falsely susceptible to cephalosporins, which don’t cross the blood brain barrier

A

Lmon

71
Q

spores indicate Bacillus or….

A

clostridium

72
Q
A

Bacillus

73
Q

Bacillus O2

A

Facultative anaerobe

74
Q

Bacillus PEA

A

growth
B. anthracis - no growth

75
Q

Bacillus beneficial applications

A

Antibiotic production
Vitamin production
Validation of sterilization methods
Insect control

76
Q

Bant primarily causes disease in…

A

ruminants

77
Q

protein antiphagocytic capsule

A

Bant

78
Q

2 toxins in Bant

A

edema toxin and lethal toxin

79
Q

Bant transmission

A

through animal products

80
Q

Most Bant infections are cases of ———- and present as …….

A

cutaneous anthrax
Painless, slow healing black ulcer called an eschar

81
Q

Toxin effects in resp. anthrax

A

Causes edema, internal bleeding, tissue damage and necrosis

82
Q

Antibiotics do not work on resp. anthrax once…

A

toxin reaches bloodstream

83
Q

Rare 3rd form of anthrax

A

intestinal anthrax

84
Q

2001 bioterror organism

A

Bant

85
Q

Bant safety procedures

A

class II biological safety cabinet

Need a class III cabinet for manipulations that may create aerosols (mincing , vortexing, cutting)

86
Q

Bant vaccine recommended for…

A

Military, lab workers with direct contact with organism, and those who work with animals and their products imported from endemic areas

87
Q

Do not use ——— to tx Bant because…

A

No penicillin due to possible inducible β-lactamase

88
Q

most common lab contaminant

A

Bcer

89
Q

2 forms of Bcer food poisoning due to toxin

A

Emetic
Diarrheal

90
Q

Most often associated with consuming fried rice at Asian restaurants

A

Bcer food poisoning

91
Q

Can cause eye infection due to trauma, especially from farm accidents

A

Bcer

92
Q

Antibiotic that separates Bcer from Bant

A

Bant is susceptible to penicillin
Bcer is resistant

93
Q

Found in animals, pigs being the main reservoir

A

Erhu

94
Q

Localized form of cellulitis caused by Erhu

A

erysipeloid

95
Q

Lesions are purplish, swollen, painful and spread slowly

40% mortality if it goes septicemic

A

Erhu

96
Q

Erhu specimen

A

Need to culture a full thickness biopsy of infected skin

97
Q

intrinsically resistant to Vanc, teicoplanin, SXT, and aminoglycosides

A

Erhu

98
Q

Lactobacillus importance

A

Important in maintaining pH of healthy female genital tract

99
Q

intrinsically resistant to Vanc, infection is very rare

A

Lactobacillus

100
Q

inhibited by SBS anticoag

A

G. vaginalis

101
Q

gold standard for dx of BV

A

GS

102
Q

shift that happens in BV

A

from good GP organisms to bad GN organisms

103
Q

GN organisms that cause BV when Lactobacillus disappears

A

Gardnerella vaginalis
Bacteroides spp.
Prevotella spp.
Mobiluncus spp.

104
Q

BV clinical sx

A

gray discharge, fishy odor with 10% KOH, pH >4.6

105
Q

BD Affirm VPIII detection threshold is set…..

A

above levels of normal flora and detects only clinically significant levels (disease state) of Gardnerella and Candida species

106
Q

BD Affirm VPIII is useful for…

A

fast results (1 hour) for pregnant women at delivery

107
Q

Complications of BV in pregnant women

A

Preterm birth
Premature rupture of the membranes (PROM)
Chorioamnionitis

108
Q

Shows similar arrangement on GS to diphtheroids

A

Arcanobacterium haemolyticum

109
Q

A. haemolyticum mimics…

A

S. pyogenes or S. anginosus

110
Q

Distinguishes Ahae from Spyo (3)

A

GS; LAP = (Spyo +); PYR = (Spyo +)

111
Q

Causes pharyngitis that can be mistaken for Strep

A

A. haemolyticum

112
Q
A

A. israelii

113
Q

A. israelii clinical significance

A

cervicofacial actinomycosis

114
Q

colonies of organisms in pus resemble hard, yellow sulfur granules

A

A. israelii

115
Q

risk factors for actinomycosis

A

dental procedures
oral surgery
IUD

116
Q

GPR genera (8)

A

Actinomyces
Arcanobacterium
Bacillus
Corynebacterium
Erysipelothrix
Gardnerella
Lactobacillus
Listeria