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Microanatomy > Methodology > Flashcards

Methodology Flashcards

1
Q

Autoradiography

A

Used to visualize synthesis of a component in a cell by using a radiolabel (isotope) tritum
H3 or C14`

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2
Q

Histochemistry/ Cytochemistry

A

Using a specialized stain to identify chemical components of cells or tissues

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3
Q

Stain for reticular fibers

A

Silver Nitrate

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4
Q

Stain for golgi bodies

A

silver nitrate

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5
Q

Stain for glycogen

A

Periodic Acid Schiff (PAS), best carmine

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6
Q

Stain for lysosomes

A

Acid phosphatase enzyme

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7
Q

Stain for lipid

A

Oil red/ sudan black (frozen)

Osmium (paraffin)

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8
Q

Stain for peroxisomes

A

Catalase Enzyme

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9
Q

Stain for mitochondria

A

Succinate dehydrogenase enzyme

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10
Q

Stain for elastic fibers

A

Weigert’s elastic stain

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11
Q

Stain for carbohydrates

A

Periodic Acid Schiff

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12
Q

Immunocytochemistry

A

Specific interaction between antigens and antibodies, is useful in identifying and localizing specific proteins and glycoproteins
Detects cancer and identifies cytoplasmic components

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13
Q

Hematoxylin Stain

A

Basic stain with a positive charge
Stains negatively charged, acidic components with blue/ purple stain
Example: nucleus and ribosomes

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14
Q

Eosin Stain

A

Acidic and negatively charged
Stains basic and positively charged components with pink stain
Example: most cytoplasmic components except for nucleus and ribosomes

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15
Q

Scanning microscope

A

Examines 3D views of the surface of cells, tissues and organs

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16
Q

Electron Microscope

A

2: scanning and transmission
0.5 m
Max magnification 400,000x
Uses electrons released from a metal

17
Q

Transmission microscope

A

In depth-exam of cell components

Fixation, embedding and staining of tissues required

18
Q

What stain do transmission microscopes use?

A

Uranyl acetate and lead citrate

19
Q

Phase contrast

A

Used for examining living, unstained cells

EX: sperm motility, dividing cells

20
Q

Confocal and Florescence Microscopes

A

Used in research labs

21
Q

Bright Field Microscope

A

Used for routine histopathological examination

Fixation, embedding and H&E staining required

22
Q

Light Microscope

A

Any structure smaller than 0.5 micrometers cannot be resolved with LM
Max magnification: 1000x
Used for tissue illumination

23
Q

What are different types of Light microscopes

A

Bright field, phase contract and confocal and florescence

24
Q

Human eye (R)

A

0.5 mm–> any object smaller cannot be seen with a naked eye

25
Q

Magnification

A

Image divided by the actual size

26
Q

Resolution

A

Smallest distance between 2 particles where they’re seen as separate objects

Microanatomy (18 decks)

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