Methodology

Question 1

Autoradiography

Answer 1

Used to visualize synthesis of a component in a cell by using a radiolabel (isotope) tritum
H3 or C14`

Question 2

Histochemistry/ Cytochemistry

Answer 2

Using a specialized stain to identify chemical components of cells or tissues

Question 3

Stain for reticular fibers

Answer 3

Silver Nitrate

Question 4

Stain for golgi bodies

Answer 4

silver nitrate

Question 5

Stain for glycogen

Answer 5

Periodic Acid Schiff (PAS), best carmine

Question 6

Stain for lysosomes

Answer 6

Acid phosphatase enzyme

Question 7

Stain for lipid

Answer 7

Oil red/ sudan black (frozen)

Osmium (paraffin)

Question 8

Stain for peroxisomes

Answer 8

Catalase Enzyme

Question 9

Stain for mitochondria

Answer 9

Succinate dehydrogenase enzyme

Question 10

Stain for elastic fibers

Answer 10

Weigert’s elastic stain

Question 11

Stain for carbohydrates

Answer 11

Periodic Acid Schiff

Question 12

Immunocytochemistry

Answer 12

Specific interaction between antigens and antibodies, is useful in identifying and localizing specific proteins and glycoproteins
Detects cancer and identifies cytoplasmic components

Question 13

Hematoxylin Stain

Answer 13

Basic stain with a positive charge
Stains negatively charged, acidic components with blue/ purple stain
Example: nucleus and ribosomes

Question 14

Eosin Stain

Answer 14

Acidic and negatively charged
Stains basic and positively charged components with pink stain
Example: most cytoplasmic components except for nucleus and ribosomes

Question 15

Scanning microscope

Answer 15

Examines 3D views of the surface of cells, tissues and organs

Question 16

Electron Microscope

Answer 16

2: scanning and transmission
0.5 m
Max magnification 400,000x
Uses electrons released from a metal

Question 17

Transmission microscope

Answer 17

In depth-exam of cell components

Fixation, embedding and staining of tissues required

Question 18

What stain do transmission microscopes use?

Answer 18

Uranyl acetate and lead citrate

Question 19

Phase contrast

Answer 19

Used for examining living, unstained cells

EX: sperm motility, dividing cells

Question 20

Confocal and Florescence Microscopes

Answer 20

Used in research labs

Question 21

Bright Field Microscope

Answer 21

Used for routine histopathological examination

Fixation, embedding and H&E staining required

Question 22

Light Microscope

Answer 22

Any structure smaller than 0.5 micrometers cannot be resolved with LM
Max magnification: 1000x
Used for tissue illumination

Question 23

What are different types of Light microscopes

Answer 23

Bright field, phase contract and confocal and florescence

Question 24

Human eye (R)

Answer 24

0.5 mm–> any object smaller cannot be seen with a naked eye

Question 25

Magnification

Answer 25

Image divided by the actual size

Question 26

Resolution

Answer 26

Smallest distance between 2 particles where they’re seen as separate objects