Manipulating Genomes

Question 1

What is the polymerase Chain Reaction used for

Answer 1

To generate many copies of DNA fragments and amplify it

Question 2

How is PCR done

Answer 2

Double stranded DNA is heated to 95C to break hydrogen bonds between bases
Primers are added and temp is lowered to 55C to allow primers to anneal
Temp raised to 75C and Taq DNA polymerase binds and extends primers using free nucleotides
Repeat for exponential growth