Lecture | Streptococcus & Enterococcus Flashcards
catalase negative gram-positive cocci in pairs or chains
streptococci
morphology: Streptococcus forms in
chain
morphology: Staphylococcus forms in
clusters
Used to differentiate Streptococcus from Micrococcaeceae
Catalase test
Streptococcus is catalase ?
catalase negative
Staphylococcus or other Micrococcus is catalase ?
catalase positive
normal flora of Upper respiratory tract and skin lesions
S,pyogenes
normal flora of Upper respiratory microbiota
S.penumoniae
Lancefield groups based on carbohydrates in the cell wall
BETA-HEMOLYTIC STREPTOCOCCI
Group A
S.pyogenes
Group B
S.agalactiae
Group C
S.dysgalactiae
Group D
Enterococcus spp. and Streptococcus bovis complex
most clinically important Lancefield group A
Streptococcus pyogenes
Virulence factors of S. pyogenes responsible for the betahemolytic pattern on blood agar plates
Streptolysin O and S
one of the most aggressive pathogens encountered in clinical microbiology laboratories.
S. pyogenes
flesh eating bacteria
S. pyogenes
oxygen-stable, nonimmunogenic, hemolysin capable of lysing erythrocytes, leukocytes, and platelets in the presence of room air. (20-22C)
Streptolysin S
it means cannot trigger immune response
nonimmunogenic
mmunogenic, capable of lysing the same cells and cultured cells, is broken down by oxygen
Streptolysin O
will produce hemolysis only in the absence of room air.
Streptolysin O
Streptolysin O is inhibited by this resulting in the absence of the development of protective antibodies associated with skin infection.
cholesterol in skin lipids
virulent factor of S. pyogenes that permits the bacterial adherence to the respiratory epithelium
Lipotechoic acid
virulent factor of S. pyogenes released and produce scarlet fever
Streptococcal Pyrogenic Exotoxins (SPEs)
occurs in association with streptococcal pharyngitis and is manifested by a rash of the face and upper trunk.
scarlet fever
The SPEs are erythrogenic toxins produced by
lysogenic strains
They are heat labile and rarely found in group C and G streptococci.
Streptococcal Pyrogenic Exotoxins (SPEs)
SPEs act as superantigens activating macrophages and T-helper cells and inducing the release of powerful immune mediators such as
(IL)-1, IL-2, IL-6, tumor necrosis factor (TNF)-alpha, TNF-beta, interferons, and cytokines
from streptococcal infection through the mouth
Streptococcal toxic shock syndrome
Streptococcal toxic shock syndrome, typified by multisystem involvement including
renal and respiratory failure, rash, and diarrhea, is a serious disease mediated by production of potent SPE.
most important virulence factor of our Streptococcus pyogenes
M protein - anti-phagocytic cell wall
anti-phagocytic and also very immunogenic
M protein
associated with rheumatic fever
Class 1M protein
Associated with glomerulonephritis
class I or II