Lab | General Concepts of Media Flashcards
Component:
› Trypticase soy agar
› Brucella Agar/Beef Heart
› 5% sheep blood
Sheep Blood (BA)
Purpose:
› Cultivation of non-fastidious microorganism
› Determination of hemolytic reactions
Sheep Blood (BA)
Component:
› Dextrose
› Pork brain
› Heart dehydrated infusions
Brain-heart infusion Agar or Broth
Purpose: Cultivation of fastidious organism
Brain-heart infusion Agar or Broth
Component:
› Peptone Base
› Enriched solution of 2%
Hemoglobin
Chocolate Agar
Purpose:
› Cultivation of fastidious organism
› Haemophilus spp.
› Brucella spp.
› Pathogenic Neisseria spp.
Chocolate Agar
Colistin (C) - disrupts GN cell membrane
Nalidixic acid (NA) – blocks DNA replication
Columbia colistin-nalidixic acid (CAN) Agar
Purpose: Selective isolation of gram+ cocci
Component:
› Peptone sources
› 5% defibrinated sheep
blood
Columbia colistin-nalidixic acid (CAN) Agar
Component:
› Mannitol – primary carbon source
› Sodium citrate & Sodium deoxycholate – bile salts that inhibit GP organisms
› Peptone base broth with glucose
Gram Negative (GN) Broth
Purpose: Selective (enrichment)
liquid medium for enteric pathogens
Gram Negative (GN) Broth
Component:
› Peptone-base sugar with bile salts, lactose, sucrose, agar salicin, and ferric ammonium citrate
› Indicator: bromthymol blue and acid fuchsin
Hektoen Enteric (HE) Agar
Purpose:
Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp. From other gram-negative enteric bacilli
Hektoen Enteric (HE) Agar
Component:
› Peptone base with lactose
› Gram-positive organisms inhibited by crystal violaet and bile salts
› Indicator: neutral red
MacConkey Agar
Purpose: Isolation and differentiation of lactose fermenting and non-lactose-fermenting enteric bacilli
MacConkey Agar
Component:
› Nutrient agar base
› Phenylethyl alcohol inhibits
growth of gram-negative
organisms
› 5% sheep blood
Phenylethyl Alcohol (PEA) Agar
Purpose: Selective isolation of aerobic gram-positive cocci and bacilli
Phenylethyl Alcohol (PEA) Agar
Component:
› Blood agar based enriched with hemoglobin and supplement B
Colistin = inhibit GN
Vancomycin = inhibit GP
Nystatin = inhibit yeast
Trimethoprim = inhibit Proteus spp.
Modified Thayer-Martin Agar
Purpose:
› Selective isolation of aerobic gram-positive cocci and bacilli ( N. gonorrhoeae & N. meningitidis)
› Supports growth of Francisella and Brucella spp.
Modified Thayer-Martin Agar
Component:
› Pancreatic digest of casein
› Soy broth
› Glucose
Thioglycollate Broth
Purpose:
› broth/semi- solid
› Supports of anaerobes, aerobes, microaerophilic, and fastidious microorganisms
Thioglycollate Broth
Component:
› Yeast extract agar w/ lysine, xylose, lactose & sacrose
Sodium deoxycholate = inhibits GN
Phenol Red = pH indicator
Ferric ammonium citrate = H2s indicator
Xylose-Lysine-Deoxycholate (XLD) Agar
Purpose:
Isolation and differentiation of Salmonella and Shigella spp. From other gram- negative enteric bacilli
Xylose-Lysine-Deoxycholate (XLD) Agar and
