Lab | General Concepts of Media Flashcards

1
Q

Component:
› Trypticase soy agar
› Brucella Agar/Beef Heart
› 5% sheep blood

A

Sheep Blood (BA)

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2
Q

Purpose:
› Cultivation of non-fastidious microorganism
› Determination of hemolytic reactions

A

Sheep Blood (BA)

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3
Q

Component:
› Dextrose
› Pork brain
› Heart dehydrated infusions

A

Brain-heart infusion Agar or Broth

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4
Q

Purpose: Cultivation of fastidious organism

A

Brain-heart infusion Agar or Broth

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5
Q

Component:
› Peptone Base
› Enriched solution of 2%
Hemoglobin

A

Chocolate Agar

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6
Q

Purpose:
› Cultivation of fastidious organism
› Haemophilus spp.
› Brucella spp.
› Pathogenic Neisseria spp.

A

Chocolate Agar

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7
Q

Colistin (C) - disrupts GN cell membrane
Nalidixic acid (NA) – blocks DNA replication

A

Columbia colistin-nalidixic acid (CAN) Agar

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8
Q

Purpose: Selective isolation of gram+ cocci
Component:
› Peptone sources
› 5% defibrinated sheep
blood

A

Columbia colistin-nalidixic acid (CAN) Agar

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9
Q

Component:
› Mannitol – primary carbon source
› Sodium citrate & Sodium deoxycholate – bile salts that inhibit GP organisms
› Peptone base broth with glucose

A

Gram Negative (GN) Broth

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10
Q

Purpose: Selective (enrichment)
liquid medium for enteric pathogens

A

Gram Negative (GN) Broth

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11
Q

Component:
› Peptone-base sugar with bile salts, lactose, sucrose, agar salicin, and ferric ammonium citrate
› Indicator: bromthymol blue and acid fuchsin

A

Hektoen Enteric (HE) Agar

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12
Q

Purpose:
Differential, selective medium for the isolation and differentiation of Salmonella and Shigella spp. From other gram-negative enteric bacilli

A

Hektoen Enteric (HE) Agar

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13
Q

Component:
› Peptone base with lactose
› Gram-positive organisms inhibited by crystal violaet and bile salts
› Indicator: neutral red

A

MacConkey Agar

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14
Q

Purpose: Isolation and differentiation of lactose fermenting and non-lactose-fermenting enteric bacilli

A

MacConkey Agar

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15
Q

Component:
› Nutrient agar base
› Phenylethyl alcohol inhibits
growth of gram-negative
organisms
› 5% sheep blood

A

Phenylethyl Alcohol (PEA) Agar

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16
Q

Purpose: Selective isolation of aerobic gram-positive cocci and bacilli

A

Phenylethyl Alcohol (PEA) Agar

17
Q

Component:
› Blood agar based enriched with hemoglobin and supplement B

Colistin = inhibit GN
Vancomycin = inhibit GP
Nystatin = inhibit yeast
Trimethoprim = inhibit Proteus spp.

A

Modified Thayer-Martin Agar

18
Q

Purpose:
› Selective isolation of aerobic gram-positive cocci and bacilli ( N. gonorrhoeae & N. meningitidis)
› Supports growth of Francisella and Brucella spp.

A

Modified Thayer-Martin Agar

19
Q

Component:
› Pancreatic digest of casein
› Soy broth
› Glucose

A

Thioglycollate Broth

20
Q

Purpose:
› broth/semi- solid
› Supports of anaerobes, aerobes, microaerophilic, and fastidious microorganisms

A

Thioglycollate Broth

21
Q

Component:
› Yeast extract agar w/ lysine, xylose, lactose & sacrose

Sodium deoxycholate = inhibits GN
Phenol Red = pH indicator
Ferric ammonium citrate = H2s indicator

A

Xylose-Lysine-Deoxycholate (XLD) Agar

22
Q

Purpose:
Isolation and differentiation of Salmonella and Shigella spp. From other gram- negative enteric bacilli

A

Xylose-Lysine-Deoxycholate (XLD) Agar and