Lecture 7

Question 1

How do you identify if your cells are iPSC cells?

Answer 1

Check their cell markers and what proteins they express

Question 2

What kind of cells are iPSC cells most like?

Answer 2

embryonic stem cells

Question 3

What is a pathway ?

Answer 3

A series of molecular actions that occur within a cell
to produce a specific product or change

Question 4

What does the Wnt/ b-catenin pathway regulate?

Answer 4

cell growth, motility, and differentiation during embryonic development

Question 5

What is the canonical pathway for Wnt/ b-catenin?

Answer 5

the pathway that is more related to cell proliferation

Question 6

What is the noncanonical pathway for Wnt/ b-catenin?

Answer 6

the pathway related to cell migration

Question 7

What is the advantage of controlling the Wnt/ b- catenin pathway?

Answer 7

We are able to try to control the cell behavior and the differentiation

Question 8

What are the checkpoint for the production of cardiomyocytes?

Answer 8

confluence and morphology

Question 9

True/False: iPSC – Pluripotent: can potentially produce any cell or tissue

Answer 9

True

Question 10

How were the ethical concerns address with embryonic stem cells?

Answer 10

by introducing pluripotent stem cells

Question 11

Who first introduced iPSC?

Answer 11

Yamanaka group (2006/2007)

Question 12

How many reprogramming factors are there in iPSC?

Answer 12

4

Question 13

What process is the key in many processes of proliferation and differentiation?

Answer 13

Wnt/ b-catenin

Question 14

True/False: A single protocol
was established for different cell types

Answer 14

False: Multiple protocols established for different cell types

Question 15

What are the pros of using iPSCs for TE?

Answer 15

-specificity (make into any cell)
-autologous transplant (patients cells)
- less ethical concerns

Question 16

What are the cons of using iPSCs for TE?

Answer 16

-cost
-tumorigenicity
- regulatory landscape (FDA?)

Question 17

Are there any stem cell approved therapies?

Answer 17

Yes

Question 18

Give some example factors that influence how the cell differentiates?

Answer 18

blood vessels, soluble factors, progenitor cells, extracellular matrix, stromal cells, etc

Question 20

What regulate the interaction
of cells with ECM?

Answer 20

Integrins

Question 21

What binds to integrins?

Answer 21

ligands

Question 22

What is chemotaxis?

Answer 22

response of cell in
direction or away from a soluble component (e.g., growth factor)

Question 23

What is the response of cell in direction or away from a soluble component (e.g., growth factor)?

Answer 23

Chemotaxis

Question 24

What is Haptotaxis?

Answer 24

response of cell in
direction or away from an
insoluble component (e.g.,
adhesion ligand)

Question 25

What is the response of cell in direction or away from an insoluble component (e.g., adhesion ligand)?

Answer 25

Haptotaxis

Question 26

What is Durotaxis?

Answer 26

response of cell in direction or away from a mechanical signal

Question 27

What is the response of cell in direction or away from a mechanical signal?

Answer 27

Durotaxis

Question 28

What are the three types of cell migration?

Answer 28

Chemotaxis, Haptotaxis, and durotaxis

Question 29

How can you make a cell relocated or migrate somewhere?

Answer 29

By adding chemical signals

Question 30

What are the different variables to consider when engineering the cell microenvironment

Answer 30

Growth factor presentation, mechanics, degradation, cell adhesion, and topography