Lecture 5 Flashcards
What is the highest level of stem cell that we might need to engineer any tissue in a person?
pluripotent
What is the highest level of stem cell that we might need to engineer a whole person?
totipotent
What are the types of cell signaling?
Autocrine, paracrine, and endocrine
What is autocrine signaling?
cell secretes a chemical or hormone that binds to receptors on the same cell
What is paracrine signaling?
the release of small molecules to influence nearby cells
What is endocrine signaling?
uses hormones to communicate between cells that are far apart
What are the sources for MSCs?
bone marrow, but also fat
MSCs _____ with age. (increase or decrease)
MSCs decrease with age
What are the issues with MSCs?
heterogeneity, donor availability
Give a few examples of key MSC Markers.
CD105, CD73, CD90, STRO-1, CD45-, CD34-, CD14-, CD19-
How do MSC cells understand what stage of development they are in?
they use the production of different proteins as markers for an understanding of when stage of development the stem cells are at
How do you read the MSC chart?
The cells should have the middle markers but none of the outside markers. If they have both, then it means that they are differentiating
True/False: Stem cells are found in almost all tissues of the body.
True
What is the purpose of HSC?
renew blood cells constantly
What is the purpose of stem cells?
maintenance of tissue as well as for repair after injury
What is the purpose of MSC?
renew many tissue and regulate many process (ex. immune)
How are iPSC produced?
by introducing a set of genes into adult cells to reprogram them into an embryonic-like state
What methods could be used to identify MSC?
Protein or cell membrane (markers)
