Lecture 6 Flashcards
Go over slide 9
How are dormancy and cancer related?
It is thought that cancer cells are dormant and become activated
What are some advantages of using MSC?
getting the cells from the patient’s bone marrow, you don’t always need to remove the cells to place them in a new area if they are in the correct place (recruit the cells to go to the needed area naturally), fewer ethical concerns/issues, can get for multiple organs, can have immunomodulation
What are some disadvantages of using MSC?
If you get it from the bone marrow (morbidity/pain) you have to drill into the patient, donor age or health may be affected, immunomodulation, limited regulation lifespan
How are cells differentiated from one another?
by the proteins they express
What is the Wnt/ b-catenin pathway?
A series of molecular actions that occur within a cell
to produce a specific product or change
What do Wnts regulate and when?
cell growth, motility,
and differentiation during
embryonic development
What is the process to go from DNA to protein?
DNA->Transcription->RNA->Translation->Protein
What is responsible for replicating the DNA?
Proteins
What are the steps of iPSC?
- Skin Biopsy
- Fibroblast outgrowth
- Fibroblast expansion
- Addition of reprogramming factors
- Picking of colonies
- Colony expansion and pluripotency QC
- Directed differentiation
What are the Yamanaka factors used for iPSC factors?
OCT4, SOX2, KLF4, MYC
Which scientific report came first: Animal clonation, iPSC, or relatively close?
Animal cloning
