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LAST CLASS > LEC 14 > Flashcards

LEC 14 Flashcards

1
Q

def? natural fluorescence found within cells

A

autofluorescene

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2
Q

def? Any particle which generates signal after passing through the laser beam

A

event

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3
Q

what is forward scatter proportional to

A

cell size

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4
Q

what is side scatter proportional to

A

internal complexity like granularity, vacuoles, or inclusions

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5
Q

T or F: neutrophils will have a higher side scatter than lymphs and monos

A

T

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6
Q

def? plot that displays a single measurement parameter

A

histogram

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7
Q

def? plot that display 2 measurement parameters

A

dot plots

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8
Q

def? ID of cells in a population through the staining and ID of specific markers using monoclonal AB conjugated with fluorochromes

A

immunophenotyping

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9
Q

def? dye which allows ID of dead cells through a reduction in cell mmb integrity

A

viability dye

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10
Q

T or F: dead cells can be used in flowcytometry

A

F

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11
Q

what kind of cells can flowcytometery ID

A

subgroups

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12
Q

what kind of lymph has granules

A

large granule lymphs

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13
Q

on a dot plot with SSC vs. FSC: top left will have ____ granules and _____ size

A

increased, small

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14
Q

on a dot plot with SSC vs. FSC: bottom left will have ____ granules and _____ size

A

decreased, small

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15
Q

on a dot plot with SSC vs. FSC: top right will have ____ granules and _____ size

A

increased, big

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16
Q

on a dot plot with SSC vs. FSC: bottom right will have ____ granules and _____ size

A

decreased, big

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17
Q

what does it mean if a flowcytometer has more lasers

A

the more lasers the more advanced

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18
Q

routine blood diagnostics and blood count is still done by what today

A

Coulter counters

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19
Q

def? measuring the physical and chemical properties of cells flowing in a stream of fluid

A

flow cytometry

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20
Q

t or F: flow cytometry can measure other things besides cells

A

T

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21
Q

what does flow cytometery use to obtain info about a cell

A
  • forward light scattered
  • side light scattered
  • fluorescence
22
Q

what can flow cytometery tell you about leukemias

A
  • morphology
  • immunophenotype
  • cytogenentics
23
Q

what component of FC? sheath fluid tank and flow cell

A

fluidics

24
Q

what component of FC? lasers and filters

A

optics

25
Q

what component of FC? FSC, SSC, and FL detectors

A

detectors

26
Q

what component of FC? ADC system and data processing system

A

electronics

27
Q

def? allows cells to be interrogated one by one by the laser beam

A

hydrodynamic focusing

28
Q

when will cells produce the max amount of optical signal

A

when the cell is in the center of the laser beam

29
Q

what kind of culture must cells from solid tissue be in to use in FC

A

single cell suspension

30
Q

how are whole blood and BM aspirates prepared for FC

A

must be anti-coagulated

31
Q

how are tissue samples and BM biopsies prepared for FC

A

culture media

32
Q

t or F: at least 85% of cells need to be alive for FC

A

T

33
Q

T or F: cells are alive on a PBS

A

F

34
Q

what are the 2 main objectives of data analysis in FC

A
  1. obtain statistics
  2. ID abnormal populations based on light scatter and staining patterns
35
Q

what does CD stand for

A

cluster of differentiation

36
Q

what kind of cell do CD molecules ID

A

leukocytes

37
Q

what is used to detect CD markers

A

monoclonal ab and fluoresces

38
Q

what are the cell markers for t helper cells

A

CD3+ CD4+

39
Q

what are the cell markers for t cytotoxic cells

A

CD3+CD8+

40
Q

what are the cell markers for b cells

A

CD19+CD20+CD3-

41
Q

what are the cell markers for NK cells

A

CD16+CD56+CD3-

42
Q

what are the cell markers for HSCs

A

CD20-CD25-CD34+

43
Q

what is another name for scatter plots

A

bi-variate plots

44
Q

what kind of marker is CD 45

A

pan-leukocyte marker

45
Q

what is the order of strongest expression of CD 45 in WBCs from most to least

A
  1. lymphs
  2. monocytes
  3. granulocytes
46
Q

t. or F: erythroid precursors are CD45 neg

A

T

47
Q

the more shift to the ____ the more intense fluorescence signal

A

right

48
Q

def? electronic boundary an operator uses to delineate cell clusters

A

gate

49
Q

the dot plot with CD45/SS helps with detection of what

A

blast

50
Q

what condition do CD55 and CD 59 belong to

A

PNH

51
Q

t or f: if a sample is more than 48 hrs old it should not be analyzed using FC

A

T

52
Q

what anti-coagulant is used on BM aspirates

A

heparin

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