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CLS 307 > Lab Quiz 2 > Flashcards

Lab Quiz 2 Flashcards

1
Q

What is the NMB staining procedure

A

3 drops of NMB
2 drops of blood
Mix well and have for 15 minutes
Incubate for 10 mins at 37C
Make 3 smears
Let it dry

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2
Q

What is not visible in a Wright stain

A

Reticulocytes
Heinz bodies
Hemoglobin H

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3
Q
A

Polychromasia

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4
Q
A

Basophilic stippling

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5
Q
A

Supravital stains - reticulocyte

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6
Q

A reticulocyte stain measures

A

Aggregates of residual ribosomes and mitochondria that form clumped granular material called reticulum

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7
Q

% retics =

A

(Number of retics/1000)*100

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8
Q

For anemia, retic index (corrected) % =

A

(% retics* hematocrit of patient)/ normal hematocrit (45%)

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9
Q

For young reticulocytes, reticulocyte production index (RPI) =

A

Retic index/maturation time in days

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10
Q
A

Blister cell

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11
Q
A

Bite cell

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12
Q
A

Heinz bodies

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13
Q

How are Heinz bodies formed

A

By damage to the hemoglobin component molecules, usually through oxidative damage by administered drugs, or from an inherited mutation

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14
Q

What are denatured Hgb due to oxidative stress

A

Heinz bodies

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15
Q
A

Hemoglobin H disease
Note: tear drops and blister cells

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16
Q
A

Supravital stain - hemoglobin H
Lots of Heinz bodies

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17
Q

Hemoglobin H is made up of

A

4 beta sheets

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18
Q

Study differences

A
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19
Q
A

Pappenheimar bodies
(Abnormal granules of iron)

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20
Q

What stain is Perl’s reaction

A

Prussian blue stain

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21
Q

What does the Prussian blue stain (perl’s reaction) demonstrate

A

Iron stores in bone marrow or sideroblasts on peripheral blood smear

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22
Q
A

Positive iron stain in bone marrow

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23
Q
A

Negative iron stain in bone marrow

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24
Q
A

Howell-jolly bodies
(Nuclear remnants) fragments of DNA

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25
Q
A

Cabot rings
(Remnants from a mitotic spindle)

26
Q
A

Rouleaux
“Roll of pennies”

27
Q

Makes cells less neg. Allowing them to touch

A

High protein gathering electrons

28
Q
A

RBC agglutination

29
Q

Name and survival rate

A

Neutrophils
1-2 days

30
Q
A

Band neutrophils

31
Q
A

Toxic granulation

32
Q
A

Dohle bodies

33
Q
A

Vacuoles

34
Q
A

Blue-green inclusions
“Death crystals”

35
Q

Name the disease and morphology

A

Iron deficiency anemia
Microcytic hypochromic

36
Q

Name the disease

A

Megaloblastic anemia
Macrocytic anemia

37
Q

Name the disease and characteristics

A

Hereditary spherocytosis
Spherocytes-variable, polychromasia
BM pushing more cells out

38
Q

Name the disease and characteristics

A

Hereditary elliptocytosis
Elliptocytes, mild anemias

39
Q
A

Eosinophil

40
Q
A

From left to right:
Segmented neutrophil
Basophil
Monocyte

41
Q
A

Basophil

42
Q
A

Monocytes

43
Q
A

Lymphocytes

44
Q

Name the disease and characteristics

A

Iron deficiency anemia
Microcytes, hypochromasia, often high platelets

45
Q

Name the disease and characteristics

A

Severe iron deficiency
Microcytic, hypochromic, elliptocytes, tear drops, bizarre forms, often high platelet count

46
Q

Name the disease and characteristics

A

Sideroblastic anemia
Can have pappenheimer bodies, one population hypochromic microcytic the other normal

47
Q

Name the cell

A

Ringed sideroblasts (bone marrow)

48
Q

Name the disease and characteristics/morphology

A

Megaloblastic anemia
Oval macrocytes, howell-jolly bodies, tear drop (dacryocytes), hyper-segmented neutrophil, Cabot ring

49
Q

Does the white count, red count, and platelets increase or decrease in megaloblastic anemia

A

Decrease

50
Q

Name the disease and characteristics

A

Pernicious anemia
Oval monocytes, tear drops, hyper-segmented neutrophils

51
Q

Name the disease and characteristics

A

Liver disease
Macrocytes, Target cells, acanthocytes
High/increased MCV

52
Q

Name the disease and characteristics

A

Hereditary pyropoikliocytosis
Elliptocytes/ovalocytes, red cell fragments, severe microcytosis

53
Q

Name the disease and characteristics

A

Thermal burns
Spherocytes, fragmented red cells, microvesicles, no Polychromasia, rapidly cleared

54
Q
A

Immune hemolytic anemia

55
Q

Name the disease and characteristics

A

Hereditary spherocytosis (post splenectomy)
Spherocytosis, polychromasia

56
Q

Name the disease and characteristics

A

Microangiopathic anemia
Schistocytes, little to no platelets, sepsis, inappropriate clotting

57
Q

Name the disease and characteristics

A

Marked eosinophilia
Drug allergies

58
Q

Name the cell and characteristics

A

Basophil
Water soluble granules can dissolve during staining
No pink granules (neutrophil)

59
Q

Name the disease and its characteristics

A

Sepsis
Toxic neutrophils, toxic granulation, vacuolization

60
Q

Name the disease and its characteristics

A

Monocytosis
Irregular shaped nuclei, light areas
Tuberculosis, listeriosis, malignancy

CLS 307 (21 decks)

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