Lab lect 3 Flashcards

1
Q

what is the giemsa stain

A

-made by gustav giemsa a chemist and bacteriologist
-mix of an acidic (eosin) and two basic dyes (methylene blue/azure B)
-specidic for phosphate groups on dna (stains anything with dna)

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2
Q

giemsa stain is used for staining for

A

-blood smear (leukocyte cells) and parasites like plasmodium, trypanosomes, microfilariae and leish

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3
Q

how is the blood collected for a giemsa stain

A

-finger, ear lobe or toe for kids

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4
Q

what is the difference between thick and thin blood films for malaria diagnosis

A

-thick: parasite detection for low parasitemia
-thin film: used for confirmation and species identification

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5
Q

thick blood film prep

A

-touch the blood drop with a clear slide or 2 drops of blood (30-40 ul)
-using the corner of another slide, spread the blood into the shape of a circle of 1-1.5 cm2

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6
Q

thin blood slide prep

A

-touch the blood drop with a clear slide
-hold the slide and with another slide at 30-45 degree angle touch the drop and wait until the blood spread along the edge of the second slide
-rapidly and smoothly push slide forward

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7
Q

difference between p vivax and p falciparum multiple infection in rbc

A

vivax: only has one ring
valciparum: common aka there are more than 1 ring

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8
Q

difference between p vivax and p falciparum parasitemia

A

vivax: low
falciparum: high

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9
Q

difference between p vivax and p falciparum ring stage

A

vivax: relatively large usually 1 chromatin dot
falciparum: may have 1 or 2 chromatin dots

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10
Q

difference between p vivax and p falciparum gametocytes stage

A

vivax: round
falciparum: sausage shaped

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11
Q

what do monocytes look like

A

kidney shaped nucleus

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12
Q

what do neutrophils look like

A

multiple lobed 3-5 nucleus

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13
Q

what do lymphocytes look like

A

one round nucleus, little cytoplasm

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14
Q

what do eosinophils look like

A

2 lobed nucleus; red granules in cytoplasm

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15
Q

what do basophils look like:

A

large blue granules in cytoplasm

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16
Q
A