Lab lect 1

Question 1

what is another name for visceral leishmaniasis

Answer 1

kala azar

Question 2

what causes visceral leishaniasis

Answer 2

leishmania donovani

Question 3

what causes cutanenous leishmaniasis

Answer 3

leish major

Question 4

what is the name of the thing in the sandfly vector

Answer 4

the promastigote

Question 4

when the parasite is in the human host what is the name of the parasite

Answer 4

the amastigotew

Question 5

here are the leish 18s ribosomal gene on the chromosome

Answer 5

chr 27

Question 6

what is the target of crispr in leish

Answer 6

18s ribosomal gene

Question 7

what is the snp on the 18s rDNA

Answer 7

1740

Question 8

what is cas9

Answer 8

crispr associated protein 9

Question 9

what is grna

Answer 9

guide rna aka spacer

Question 10

what is a pam

Answer 10

protospacer adjacent motif

Question 11

what is the snp

Answer 11

single nucleotide polymorphism

Question 12

once… is activated by a specific grna, it will continue to cut ssdna

Answer 12

cas12a

Question 13

what do we use for gene identification diagnosis

Answer 13

cas12a

Question 14

what is the special thing about cas12a

Answer 14

it uses grna, cuts dna and activates a second activity which is a transcleavage thing and then cas12a chomps all that ssrna

Question 15

how do we get fluorescence qith cas12a

Answer 15

it is becayse there is a quencher on one side of the ssrna and a fluorescence thing on the other
-with the trans cleaveage it cuts the ssrna and then there is no more quenching and you get fluorescence