Lab 8 Digestive Flashcards
What is digestion
process of breaking down food molecules that can be absorbed.
what is ingestion
food into mouth
what is propulsion
food moves through GI tract
what is mech. digestion
physical breakdown of food into smaller pieces
what is chem. digestion
breakdown of bonds in food molecules
what is secretion
release of chem/enzymes into the lumen go GI tract
what is absorption
movement of nutrients across mucosa into blood/lymph
what is defection
elimination through rectum/anus
What are the 4 layers of the GI tract
1) Mucosa (3 layers)
2) Submucosa
3) Muscularis externa (2 layers
4) Serosa/ visceral peritoneum
what are the the 3 layers of the mucosa from deep to superficial
1) mucosa epithelium (stratified squamous or simple columnar)
2) Lamina propria (areolar CT)
3) muscular mucosae (thin layer of smooth muscle)
what is submucosa made of & what does it house
- dense irreg Ct
- houses vasculature & nerves
what are the 2 layers of muscularis externa
Circular (inner)
Longitudinal (outer)
what is peristalsis
GI movement in 1 direction by longitudinal muscles
what is segmentation
GI movement in both directions by circular muscles
what is the serosa made of
areolar CT & simple squamous
-mesentaries attach
what are mesenteries
double layer of peritoneum
anchor most of the organs in the abdominopelvic cavity to parietal peritoneum
where is greater omentum
drapes over top of the coils of the small intestine
where is the lesser omentum
stabilizes position of stomach
what does the mesentery proper do
binds sm. intestine to post. ab wall
what does the mesocolon do
binds lrg. intestine to post. ab. wall
what does the falciform ligament do
stabilizes position of liver
what is the tissues of the mouth & pharynx
stratified squamous
what is the function of the oral cavity
food is analyzed (sensory receptors for gustation) & lubricated. Digestion is initiated
- Mastication for physical digestion
- soluabalizing to form bolus
- Lingual lipase -enzymatic of lipids
- salivary amylase - enzymatic of starch
what is the function of the tongue
- manipulate food into bolus
- papillae grip food
- move food to back of mouth
where are taste buds located
between papillae
how many adult & baby teeth
(32 adult) (20baby)
how many incisors & what do they do
cut (2 sets)
how many canines & what do they do
tear/perice (1 set)
how many premolars & what do they do
crush (2 sets)
how many molars & what do they do
(3 sets) grind
what does the lingual frenulum do
attaches tongue to bottom of oral cavity
what is the function of the esophagus
move bolus to the stomach
the esophagus passes through which 2 ventral body cavities
thoracic & peritoneal
what is esophageal hiatus
opening in diaphragm that esophagus passes though on it’s way to ab. cavity
what lines the esophagus
stratifies squamous
what kind of digestion occurs in the stomach
- enzymatic digestion of protein by pepsin
- mechanical digestion vis churning
what tissue lies the stomach
-simple columnar
what does the mucous neck cells of the stomach do
secrete mucous
what do the parietal cells of the stomach do
secrete HCL
what do the cheif cells of the stomach do
secret pepsinogen - activated into pepsin by HCL
what do the G cells do
secret gastrin (stimulates secretion of gastric secretions & increases peristalsis)
What are the 3 stuctures in the Small intestine that increase SA
- Plicae circularis
- Villi
- microvilli
what do plicae circularis do
they are large folds of mucosal membrane
what are villi
finger like projections of mucosal surface
what are microvilli
“brush boarder” part of villi cells
what are the parts of the villus
lacteal - absorbs fat
capillary plexus
intestinal crypt - secretes digestive enzymes (alkaline)
what is the mucosa epithelium of the small intestine made of
simple columnar
what happens in the duodenum
part of small intestine
hepatopancreatic ampulla delivers pile & pancreatic juice
-Function = neutralizes chyme, chemical digestion
what is phyloic sphincter
controls stomach chyme entering duodenum
what is the cardiac sphincter
prevents acidic contents of stomach from splashing into esophagus
what does the jejunum do
chem. digestion & absorption
what does the ill do
absorb nutrients & H20
what is the function of the lrg instestine
H20 reabsorption compassion & starch absorb some vat's many bacteria that protect, digest, & stimulate immune system produce Vit K & vit. B
what are movements of the lrg intestine called
mass peristalsis
what is the large intestine lined with
goblet cells that produce mucus
simple columnar
what is the sigmoid colon
“S” shape part right before the rectum
what is the teninae coli
reduced layer of longitudinal muscle (only 1 line)
what are haustra
form the pockets & puckers of intestinal all
what is the internal anal sphincter made of
smooth muscle.
what is the external anal sphincter made of
skeletal muscle
what does the elevator ani musc. do
supports pelvic organs, prevents urinary incontinence
what is the exocrine function of the pancreas
secretes a basic solution that neutralizes the duodenum from acing cells to contribute to chemical digestion
what is the function of the liver
- metabolic factory & store
- produces bile
- storage or ire & fat soluble vitamins
- breakdown RBC, hemoglobin & antibodies
- synthesis of plasma proteins
structures of liver
portal triad & central hepatic venules
gallbladder function
stores & concentrates bile
what are enzyme
specific class or proteins that lower activation energy of rxn’s. They are biological catalysts that change the rate of the rxn. without being altered or consumed
what is a substrate
substance that an enzyme acts on. Bind to a specific region on the enzyme called the ACTIVE SITE. The enzyme changes shape slightly. The INDUCED FIT results from an enzyme substrate complex which facilitates the substrate into the product.
what is salivary amylase
used to investigate substrate specificity. Hydrolyzes covalent bonds between glucose in certain polysaccharides.
what are polysaccharides
differ in the amount of branching & type of covalent bond in glucose molecules. Not all plant polysaccharides can be digested. They’ll be excrete in fees if they arne’t digested.
what is benedicts reagent
It is used to monitor the products of salivary amylase reaction. It produces coloured precipitates in the presence of monosaccharides & some disaccharides after being heated. Strong base
what does it mean if benedicts reagent has a blue precipitate
no sugar present
what does it mean if benedicts reagent has a orange/yellow precipitate
glucose of other mono/dissacharides are present
What are environmental controls
they ensure that the changes observed are due to the variable being tested.
Seves as a basis for comparison in the interpretation of experimental results.
what is a negative control
produces no effects ( when no effect is expected) it demonstrates a negative result
what is a positive control
it shows positive result. Uses treatment know to produce an effect
What was the purpose of the salivary amylase starch digestion experiment
we wanted to know if salivay amylase can hydrolysis all polysaccharides. We found out that it cannot digest cellulose.
each enzyme has an optimum ph for binding…
usually 6-8
what is buret reagent used for
to test for the presence of proteins & peptides
what does blue for buiret reagent mean
no peptides or proteins
what does purple mean from buiret. reagent
proteins
what does pink from buiret. reagent mean
peptides
what is pepsin’s optimal ph
acidic
enzyme + Substrate -> enzyme-substrate complex ->
enzyme & product
activity of enzyme is affected by…
Temp, Ph, inhibitors, activators, cofactors
what is the action of the digestive enzyme
brings about hydrolysis
what is hydrolysis
chemical process where water is added across a covalent bond resulting in splitting or lysis of molecule
-This happens to proteins, cards & lipids
what is salivary amylase secreted by
serous cells of salivary glands
what is the substrate of salivary amylase
starch
what is the product of salivary amylase & starch
disaccharides (ex. maltose)
what is pancreatic amylase secreted by
secreted by acinar cells of pancreas
what is the substrate of pancreatic amylase
starch
what is the products of pancreatic amylase & starch
disaccharides (ex. maltose)
what are disaccharidases secreted by?
epithelium of small intestine
what is the substrate of disaccharidases
disaccharides
what is the product of disaccharidases & disaccharides
monosaccharides
what i pepsin secreted by
chief cells in the stomach
what is the substrate of pepsin
proteins
what is the product of pepsin-protein complex
peptides
What secretes chymotrypsin, trypsin, and carboxypeptidases (Into duodenum)
acing cells of the pancreas
what is the substrate of chymotrypsin, trypsin, and carboxypeptidases
starch
what is the product of cymotrypsin, trypsin, and carboxypeptiases and starch
disaccarides
what secretes dipeptidases & peptidases
epithelium of sm. intestine
what is the substrate of dipeptidases & peptidases
dipeptides
what is the product of dipeptidases & peptidases
amino acids
what secretes lipase
lingual glands & acing cells are pancreas
what is the substrate of lipase
triglycerides
what is the product of lipase & triglycerides
3 fatty acids & glycerol, or 2 fatty acids & 1 monoglyceride
what secretes nucleases
secreted by acinar cells of pancreas
what is the substrate of nucleases
DNA & RNA
what is the product of nucleases
nucleotides
what secreted nucleosidases
epithelium of small intestine
what is the substrate of nucleosidases
nucleotides
what is the product of nucleosidases & nucleotides
components of nucleotides (Nitrogenous base, ribose, deoxyribose + sugar)
when are pipettes used
for volumes less than 10ml
-always check for cracks first
what is a serological/blow out pipette
expel all fluid
what is a mohr pipette
tip not expelled