JVD 2010 #1 - Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs Flashcards
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
What was performed in this study?
Sectioned teeth, dissolved tissues with bleach and then performed SEM on the pulp chamber surface of the dentin at 3 locations - crown, apically in root and coronally in root..
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
What was significant about the methodology?
Did not use EDTA or phosphoric acid - so should be no change in tubule diameter
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
Describ Type I dentin.
smooth surface with dentinal tubules regularly distributed
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
What type of dentin is this?
Type I
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
Describe Type II dentin
Irregular surface (wavy, globular) with dentinal tubules regularly distributed
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dogs
What type of dentin is this?
Type II
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
Describe Type III dentin
very irregular surface, formed by dentinal tubule projections of variable thickness, creating a disordered mesh.
irregular, elongated shaped openings among these projections corresponding to dentinal tubules with some areas devoid of dentinal tubules
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
What type of dentin is this?
Type III
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
What type of dentin is this?
Type III
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
What difference was found between tubule size and tubule density between young and old dogs?
Tubule density showed no significant difference,
Tubule size was larger in younger dogs
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
What change was found between coronal region and apical regin in number of tubules?
fewer tubules in the apical region
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
How many tubules were there on average per mm squared?
70,000
Note this is higher because tubules are closer together on the pulp side.
According to Sabás Z. Hernández, DVM, PhD; Viviana B. Negro, DVM, PhD; Rodrigo H. Paulero, DVM; Paula G.Toriggia, DVM; Daniela M. Saccomanno, DVM
in Scanning Electron Microscopy of Pulp Cavity Dentin in Dog
Approximately what diameter are the dentinal tubules in this location?
1.5-2 um