Immunological testing Flashcards
1
Q
Which immunoglobin is dominant in a secondary response?
A
IgG
2
Q
What does ELISA stand for?
A
Enzyme-linked immunosorbent assay
3
Q
What does an ELISA test rely on?
A
Antibody-antigen interaction
4
Q
What are the benefits of an ELISA test?
A
- quantifiable
- quick
- reproducible
- inexpensive
5
Q
Describe the steps of an ELISA test
A
- Coat the plate wells with antigen
- Add test sera samples - will bind in positive animals
- Add enzyme tagged anti-immunoglobulin (secondary antibody) that will bind to positive antigens
- Add substrate and look for a colour change/light signal
- in between each step wash the plate to remove unbound agents
6
Q
What is an end point titration?
A
A continual dilution of a sample until you can no longer measure antibody
A sample that requires more dilutions had an original higher conc of antibody
7
Q
What determines the size of immune complexes?
A
- Relative amounts of antigen and antibody
- Number of binding sites on the antigen
- Class of antibody
8
Q
What is the biggest advantage of using immunofluorescence?
A
More sensitive so can detect lower quantities of what we are looking for
9
Q
What is the most common rapid serological test?
A
Snap tests
10
Q
Describe how to use a snap test
A
- Add a sample at one end and look for lines at the other end
- First line shows that the test has worked
- Relies on migration of the serum
- If there are no antibodies then binding wont occur
