HOSPITAL ACQUIRED INFECTIONS Flashcards
WHAT IS THE TERM USED FOR INFECTIONS ORIGINATING IN A HOSPITAL?
NOSOCOMIAL INFECTIONS
OTHER NAMES FOR NOSOCOMIAL INFECTIONS?
HOSPITAL ACQUIRED INFECTION (HAI)
HEALTHCARE ASSOCIATED INFECTIONS (HCAI)
DEFINITION OF HOSPITAL ACQUIRED INFECTIONS?
INFECTIONS THAT ARE NOT PRESENT AT THE TIME OF ADMISSION BUT OCCUR IN A PATIENT DURING ADMISSION TO A HOSPITAL OR HEALTHCARE FACILITY
COMMUNITY ACQUIRED INFECTIONS ARE?
INFECTIONS CONTRACTED OUTSIDE OF A HOSPITAL, IN THE COMMUNITY
ACCORDING TO NICE, HOW MANY PATIENTS EVERY YEAR ACQUIRE AN INFECTION AFTER TREATMENT WITHIN NHS?
300 000
IN DEVELOPING COUNTRIES, ONE IN HOW MANY HOSPITALISED PATIENTS WILL ACQUIRE A HAI?
1/10
FINANCIAL BURDEN OF HAI ON THE NHS?
1 BILLION POUNDS PER YEAR
WHAT KIND OF BACTERIA IS STAPHYLOCOCCUS AUREUS?
IT IS A COMMENSAL AND A PATHOGEN, A GRAM POSITIVE COCCUS, SEEN IN TYPICAL GRAPE LIKE (STAPHYLO) CLUSTERS
WHICH BACTERIA USUALLY FORMS TYPICAL GRAPE-LIKE CLUSTERS?
STAPHYLOCOCCUS AUREUS
EXPLANATION OF THE NAME: STAPHYLOCOCCUS AUREUS?
STAPHLYO MEANS ‘GRAPE-LIKE’ AND DESCRIBES CLUSTERS THIS BACTERIA USUALLY FORMS
COCCUS MEANS: having a spherical, ovoid, or generally round shape
AUREUS: ADDED TO THE NAME OF THIS SPECIES DUE TO THE GOLDEN (AURUM) PIGMENT IT PRODUCES
WHAT IS S. AURUS BASED ON ITS OXYGEN USAGE?
A FACULTATIVE ANAEROBE (CAN SURVIVE WITH AND WITHOUT OXYGEN)
HOW CAN S. AURUS BE DIFFERENTIATED FROM OTHER STAPHYLOCOCCI?
IT PRODUCES COAGULASE (AN ENZYME THAT CAUSES BLOOD CLOTTING), WHILE THE OTHER SC ARE COAGULASE-NEGATIVE
DESCRIVE S. AUREUS AS COMMENSAL BACTERIA:
S. AUREUS IS A MEMBER OF HUMAN MICROBIOTA; 30% OF POPULATION ARE CARRIERS OF IT AND HAVE IT IN THEIR NARES (NOSTRILS) AND THE SKIN WHERE IT DOESN’T CAUSE INFECTION
% OF PEOPLE WITH COMMENSAL S. AUREUS?
30%
WHAT ARE ADHESINS?
PROTEINS THAT AID BACTERIAL ATTACHMENT TO THE HOST CELL SURFACE
EXAMPLES OF ADHESINS?
FIBRINOGEN BINDING PROTEINS (Fnbp)
CLUMPING FACTOR A
HOW DOES FIBRINOGEN BINDING PROTEIN (Fnbp) BIND HOST CELL RECEPTORS?
IT BINDS TO INTEGRIN PROTEINS ON HOST EPITHELIAL CELLS
SOME OF THE KEY PROCESSES BACTERIA PERFORM DURING INFECTION ARE:
- BACTERIAL ADHESION
- TISSUE INVASION
- BIOFILM FORMATION
- INTRACELLULAR SURVIVAL
IN WHICH CASES DOES S. AUREUS USUALLY INVADE AND CAUSE INFECTION?
WHEN THERE IS A BREACH OF BARRIER, I.E. A CUT IN THE SKIN OR THE SKIN BARRIER OR DAMAGED MUCOSA
MECHANISMS NEUTROPHILS USE TO PHAGOCYTE BACTERIA AND KILL THEM?
- PRODUCTION OF REACTIVE OXYGEN SPECIES (ROS)
- MYELOPEROXIDASES
- FORMATION OF NEUTROPHIL EXTRACELLULAR TRAPS (NETs)
WHAT ARE BIOFILMS?
BACTERIAL COMMUNITIES OR AGGREGATES THAT ARE BOUND TOGETHER BY AN EXTRACELLULAR MATRIX
WHERE DOES S. AUREUS USUALLY FORM BIOFILMS?
- ON TISSUES E.G. WOUNDS
- ON MEDICAL DEVICES LIKE CATHETERS
SOME OF THE INFECTIONS CAUSED BY S. AUREUS?
- SKIN AND SOFT TISSUE INFECTIONS (SSTI)
- BONE AND JOINT INFECTIONS (E.G. OSTEOMYELITIS)
- INFECTIOUS OF THE HEART VALVES (INFECTIOUS ENDOCARDITIS)
- PULMONARY INFECTIONS, E.G. PNEUMONIA
- MEDICAL DEVICE OR IMPLANT RELATED INFECTIONS
- BACTEREMIA OR BACTERIA IN THE BLOOD
INFECTIOUS ENDOCARDITIS IS A LIFE THREATENING INFECTION OF HEART VALVES WHICH CAN BE CAUSED BY HAI RESULTING FROM WHICH PATHOGEN?
S. AUREUS
SOME ANTIBIOTICS S. AUREUS HAVE DEVELOPED RESISTANCE TO AND EXPLAIN:
- BETA-LACTAM ANTIBIOTIC PENICILIN (S. AUREUS PRODUCED BETA-LACTAMASES THAT INACTIVATED IT)
- BETA LACTAMASE RESISTANT ANTIBIOTICS METHICILIN AND OXACILIN (S. AUREUS DEVELOPED A NEW STRAIN: METHICILIN RESISTANT S. AUREUS AKA MRSA)
- VANCOMYCIN (ANTIBIOTIC USED AGAINST MRSA, NEW STRAIN OF THE BACTERIA DEVELOPED: VANCOMYCIN INTERMEDIATE RESISTANT S. AUREUS AKA VISA OR VANCOMYCIN RESISTANT S. AUREUS AKA VRSA
WHAT IS MRSA, WHERE DOES THE NAME COME FROM AND WHAT ARE THE TYPES?
MRSA: METHICILIN RESISTANT S. AUREUS, A TYPE OF S. AUREUS THAT DEVELOPED THE RESISTANCE TO ANTIBIOTIC METHICILIN (METHICILIN WAS USED WHEN S. AUREUS DEVELOPED RESISTANCE TO PENICILIN BY USING BETA-LACTAMASE)
CA-MRSA: COMMUNITY ASSOCIATED
HA-MRSA: HOSPITAL AQUIRED
WHAT IS VANCOMYCIN?
A GLYCOPEPTIDE ANTIBIOTIC THAT INHIBITS CELL WALL SYNTHESIS WHICH IS USED AGAINST MRSA
WHAT MAKES IT CHALLENGING TO TREAT S. AUREUS INFECTIONS?
- HIGHLY DRUG RESISTANT STRAINS OF THE BACTERIA
- HIGH RATES OF RECURRENCE THANKS TO BACTERIAL BIOFILM FORMATION WHICH IS HARD TO COMPLETELY ‘WASH AWAY’
HIGHLY RESISTANT STRAINS OF S AUREUS ARE INCREASING OR DECREASING?
INCREASING
NAME SOME S. AUREUS RESISTANCE MECHANISMS AND WHICH ANTIBIOTIC THEY’RE USED AGAINST?
- MODIFICATION OF THE DRUG TARGET (E.G. VANCOMYCIN, BETA LACTAMS LIKE PENICILIN)
- ENZYMATIC INACTIVATION OF THE DRUG (E.G. USING BETA LACTAMASE TO INACTIVE PENICILIN)
- ACTIVE EFFLUX OF THE DRUG (ATP DEPENDENT PUMPING OUT OF UNWANTED SUBSTANCES)