Histology Test 2-w18 Flashcards
What does enamel cover
Anatomical crown
Composition of enamel
96% inorganic material MAINLY HYDROXYAPETITE
3% water
1% organic ENAMELIN
The hardest calcified/mineralized tissue in the human body
Enamel
Is enamel avascular
Yes it is non vital- no nerves
Colour of enamel
Translucent, greyish/bluish white
**BUT appears yellow due to underlying dentin
The process of enamel matrix formation and mineralization
Amelogenesis
Enamel matrix is ____ derived from _____ of the enamel organ
Ectodermal ; inner enamel epithelium
Anelogenisis occurs during what stage of tooth development
Appositional (formation of enamel/dentin matrix)
Enamel matrix is initially composed of protein, carbohydrates and small amounts of calcium hydroxyapatite but DOES NOT CONTAIN
Collagen
In order for Amelogenesis to occur what must be present
Dentin
Enamel is first formed on
Cusp/tip
Enamel matrix is produced by?
Secreted by?
Produced by ameloblasts; secreted by tomes process
Ameloblasts are not differentiated on what area?
Therefore there is no enamel on ??
Root area; therefore no enamel on roots
Enamel mineralization is
Complete and immediate
What pumps calcium hydroxyapatite into the forming enamel matrix?
Ameloblasts
When ameloblasts are finished they become part of the reduced enamel epithelium fusing with
The oral mucosa
Ameloblasts are lost post tooth eruption; therefore
No new enamel is deposited
A newly erupted tooth will continue to?
Mineralized post eruption (minerals from saliva)
Developmental disturbances: What is an enamel pearl
Small, spherical enamel projections on root surface
Developmental disturbances: what is enamel dysplasia
Faulty development of enamel
What causes deep pits and fissures
Ameloblasts back into one another during apposition; cutting off their source of nutrition
= incomplete maturation of enamel matrix
What can be placed to seal off deep pits and fissures
Sealants
Enamel rods are composed of (3)
Head- enamel rod core
Tail- interrod enamel
Rod sheath- microscopic spaces btwn rods
How are enamel rods aligned?
What is the exception
Perpendicular to the DEJ
EXCEPTION: in the cervical regions of permanent teeth
Shape of enamel rods?
Key hole shaped
What PATTERN are rods aligned in
Interlocking pattern
-this adds strength and durability
What are lines of retzius
hint growth lines
Series of bands on the enamel rods
Represent incremental growth lines that extend from dej
What is neonatal line
Pronounced line of retzius marked by trauma/stress experienced by ameloblasts at birth
What is nasmyths membrane
Primary enamel cuticle- delicate membrane covers entire crown of newly erupted tooth
(Green or yellow easily worn away)
What are hunter shreger bands
Change in the direction of rods
What is perykimata
(Cervical 2/3rds of crown) Raised grooves on enamel surfaces- represent line of retzius externally
What is enamel spindles
(Found beneath the cusps and incisal tips of teeth)
Odontoblastic processes (sentinel tubules) that passed across dej and became trapped
What are enamel tufts
Small dark “brushes”
Represent areas of less mineralization
What is enamel lamella/lamellae
*hint transverse cracks
Partially mineralized vertical sheets of enamel matrix
-transverse cracks from occlusal surface of enamel to dej
Defects of enamel: hypocalcification
Chalky areas
Enamel didn’t form properly due to mineral deficiency (Ca)
Defects of enamel: decalcification
Decalcified chalky areas due to acids or poor oral hygiene
Defects of enamel: dental fluorosis
Mottled enamel
Exposure to more than 2ppm for 1st 8 years
Loss of enamel: attrition
Wearing away of tooth structure from ordinary forces of occlusion (tooth to tooth contact)
Loss of enamel: abrasion
Abnormal wear caused by friction due to excessive tooth brushing or use of abrasive toothpastes
Loss of enamel: erosion
Tooth structure lost through chemical means : Acid reflux, acidic foods/drinks, vomiting
Loss of enamel: abfraction
Wedge shaped legions
Loss of tooth structure at the cervical neck area of teeth due to compressive and tensile forces during tooth flexure
Denton makes up
The bulk of the tooth
T/F. Denton is avascular and vital; has nerves
True
Denton is ___ in colour
Yellow
Mature dentin composition
70% inorganic (calcium hydroxyapatite)
20% organic
10% water
Crystals in dentin are “_____” like and ____ than those in enamel
“Plate like” and are smaller
The process of dentin formation
Dentinogenesis
Denton forms from the ___ of the tooth germ
Dental papilla (mesenchymal)
Predentin is the initial layer of dentin matrix laid down by Odontoblasts containing
Collagen fibers
As organic matrix forms, Odontoblasts move away from the dej producing
Predentin in layers (apposition)
Apposition of the dentin occurs throughout the life of a tooth, why?
Odontoblasts remain in the tooth along the outer pulpal wall
Maturation of dentin or mineralization of predentin occurs when
Soon after its apposition
What forms during the primary phase of dentin maturation that allows for expansion and fusion
Hydroxyapatite crystals form as globules in the collagen fibers of primary dentin
Describe secondary phase of dentin maturation
Further mineralization (secondary dentin) will occur as globules form in partially mineralized areas
What is mantle dentin
Outermost layer
First predentin
What is circumpulpal dentin
Surrounds outer layer of the pulp
Bulk of the dentin
Primary dentin is present before completion of the
Apical foramen
After completion of the apical foramen what is present
Secondary dentin
forms throughout the life of the tooth!
Tertiary dentin is aka
Reparative dentin
Dentin Mineralization occurs by
Budding
Formation and calcification start at the
*hint same as enamel
Cusp
Small odontoblastic buds provide location of crystallizations which join and grow in _____ process to form___
3 dimensional process to form mantle dentin
Long tubes in the dentin that extend from the DEJ to the outer wall of pulp
Dentinal tubules
Dentinal tubules contain (3)
Odontoblastic process
Dentinal fluid
Sensory (afferent) axon (pain)
What is pertibular dentin
Wall of the tubules
What is intertubular dentin
In between the tubules (main body of dentin)
Dentinal tubules may become open due to caries, recession, attrition and cavity preparation causing
Dentinal hypersensitivity
What may trigger dentinal hypersensitivity (4)
Thermal
Mechanical (ultrasonic)
Dehydration
Chemical exposure ( acidic foods, teeth whitening)
What is the hydrodynamic theory of dentinal hypersensitivity
Pain is due to changes in the dentinal fluid associated with the odontoblastic process
How can we help clients with dentinal hypersensitivity
Desensitizing toothpastes
Fluoride varnishes
Dentin histology: What is the incremental lines of Von ebner
*growth lines
Similar to lines of retzius in enamel
Dentin Histology: what is tomes granular layer
Hint spotted areas
Found in ROOT AREA
Thin hypomineralized spotted areas giving it a granular appearance
What is dentinogenesis imperfecta?
Is it hereditary?
Shiny opalescent dentin
Hereditary
Dentinal defects: empty tubules resulting from loss of the Odontoblastic processes or odontoblast death
Dead tracts
Dentinal defects: sclerotic dentin
Form of tertiary dentin
Aka transparent dentin
Often found in areas with chronic injury of caries
Pulp is connective tissue derived from the
Dental papilla
What are the 4 pulp zones
Odontoblastic zone
Cell free zone/ zone of weil
Cell rich layer
Pulp core
Pulp zones: the Odontoblastic zone is closest to the dentin and consists of
Cell bodies of Odontoblasts
Capable of forming secondary/tertiary dentin
Pulp zones: cell free zone contains ___ cells than Odontoblastic zone
Fewer
-Nerve and capillary plexus located here
Pulp zones: cell rich layer contains many cells but fewer than what zone
Odontoblastic zone
-extensive vascular supply
Pulp zones: pulp core is the ___ of the pulp chamber
Centre
-major vessels and nerves similar to cell rich layer
Blood supply of pulp: large ____ vessels. Nutrition is obtained through?
Large cylindrical vessels
Nutrition is obtained through the tubules and their connection to the Odontoblasts
Nerve supply of pulp: cell bodies of afferent axons lie in the
Dentinal tubules
Nerve supply pulp: changes in what 3 things are perceived ONLY as PAIN
Temperatures
Vibrations
Chemical changes
What is pupal mode of entry
Apex
Lateral/accessory canals
What is pulpitis
Injury to the pull from caries, cavity prep, or other trauma. May result in pulpal infection or periapical abscess
What can happen to the pulp with age?
Vascularity and cells decrease
CT fibers and pulp stones (denticles) increase
What does the periodontium consist of
Consists of cementum, alveolar bone and PDL
Function of the periodontium
- supports the tooth in its relationship to the alveolar bone
What is cementum
Specialized connective tissue
Can cementum form throughout the life of a tooth
Yes
Where is cementum the thickest
Where is it the thinnest
Thickest at apex/interradicular areas
Thinnest at CEJ
What is the function of cementum
Covering for the root surface
Mechanical attachment (PDL to bone)
Compensates occlusal wear/attrition
Where is cementum located
On top of the tomes granular layer in dentin
Cementum forms shortly after the
Disintegration of HERS (hertwigs epithelial root sheath)
Cementum forms from
Undifferentiated cells of the dental sac
After HERS disintegrates, undifferentiated cells of the dental sac contact what and differentiate into what
Contact the root dentin surface
Differentiate into cementoblasts
Cementoblasts spread over the ____ and start laying down ____
Spread over the root dentin and start laying down cementoid
Cementoblasts can also become trapped in the cementum. These cells are called
Cementocytes
Cemetogenesis: once the cementoid is formed and reached its proper thickness it starts to
Calcify
Formation of cementum is
ONGOING