Golgi Apparatus Flashcards
Delivery of protein
- protein synthesised
- amino acids at C-terminus directs protein to location or synthesised as it is being delivered
Golgi discovery
by Italian Camillo Golgi in 1898
Golgi being the site of carbohydrate synthesis
in plants - pectin and hemicellulose
in animals - proteins decorated with sugar sidechains
importance of sugar side chains
added within Golgi
for function of a protein added by glycocyltranferase
sorts and dispatched proteins made in the ER
function of secretory vesicles
package proteins to deliver to plasma membrane
fuses with membrane and secrete to extracellular space
location of cis and trans faces
cis and trans face either side of medical cisternae
face opposite sides of Golgi
sides of Golgi contains….
different enzymes and function
movement of vesicles (process)??
dynamic process
movement of vesicles method
- vesicles budded off from ER
- fuses into membrane of Golgi
- protein passes into Golgi and transported to different location
recycling of protein
back to Golgi and retrieved by ER
retrograde transport done by COPII
ER to Golgi method
- COPII coat buds off by vesicles
- vesicles shed COPII coat and fuse = vesicular tubular cluster
- fuses with cis Golgi
2 ways in which protein may move through Golgi
- moves between cisternae and reach trans Golgi network and release
- vesicle binds to cis Golgi and moves along network
COPII function
recognise protein embedded in membrane
recruit protein with exit/transport signals
Kdel receptor
retrieves proteins back to ER
Kdel receptor method
- C-terminus and mechanism for protein to be retained within ER
- recognises protein in wrong place and binds to receptor
- packages in vesicles in retrograde transport
