Glucose Non Fermenting Gram Negatives

Question 1

Pseudomonas aeruginosa

Answer 1

beta hemolytic
possible metallic sheen or mucoid
grape or frito odor
pigment production - pyoverdine, pyocyanin, pyorubin

Question 2

Pseudomonas aeruginosa ID

Answer 2

oxidase (+)
arginine (+)
motility (+)
growth 42C (+)
nitrate (+)

Question 3

Alginate virulence factor

Answer 3

adhere and form biofilms

Question 4

Pili virulence factor

Answer 4

surface appendages that adhere organisms to cells

Question 5

Enterotoxin virulence factor

Answer 5

leads to diarrhea

Question 6

LPS virulence factor

Answer 6

produce endotoxin causing DIC, shock, fever

Question 7

Exotoxin virulence factor

Answer 7

tissue destruction, inhibition of protein synthesis

Question 8

Phospholipase virulence factor

Answer 8

destroys pulmonary surfactant and cytoplasmic membrane

Question 9

Elastase virulence factor

Answer 9

disrupts neutrophil activity

Question 10

Pyocyanin virulence factor

Answer 10

siderophore - strips iron from the host, disrupts respiratory cilia and causes damage to tissue

Question 11

Pseudomonas infection

Answer 11

swimmers ear

Question 12

Pseudomonas stutzeri ID

Answer 12

produces pyorubin pigment
Oxidase (+)
arginine (+)
motility (+)
growth 42C (+)
TSI K/K
nitrate (+)

Question 13

Stenotrophomonas maltophila

Answer 13

creamy pale yellow to lavender grey colonies
lysine (+)
motility (+)
TSI K/K

Question 14

Alcaligenes faecalis

Answer 14

may detect fruity odor
greenish discoloration
spreading colony edge

Question 15

Alcaligenes faecalis ID

Answer 15

oxidase (+)
motility (+)
growth 42C (+)
TSI K/K
nitrite (+)

Question 16

Acinetobacter baumannii ID

Answer 16

growth 42C (+)
TSI K/K
combat associated infections

Question 17

Burkholderia cepacia ID

Answer 17

oxidase (+)
lysine (+)
motility (+)
TSI K/K

Question 18

Burkholderia cepacia media

Answer 18

PCM
OFPBL
BCSA

Question 19

Burkholderia pseudomallei ID

Answer 19

oxidase (+)
arginine (+)
motility (+)
growth 42C (+)
TSI K/K
nitrate (+)
nitrite (+)

Question 20

Burkholderia pseudomallei

Answer 20

Vietnamese time bomb

Question 21

Elizabethkingia

Answer 21

oxidase (+)
TSI K/K
indole (+)
nitrite (+)

Question 22

Elizabethkingia infection

Answer 22

highly infectious in neonates

meningitis with 55% mortality rate