Bacterial Classification And ID/Gram Positive Cocci Flashcards
Eukaryotes ribosomes
80S
Prokaryotes ribosomes
70S
CNA agar
colistin solubilzes outer cytoplasmic membrane of gram neg bacteria
nalidixic acid blocks DNA synthesis
PEA agar
phenylethyl alcohol alters membrane permeability allowing influx and efflux of normally blocked substances
Staph aureus ID
coagulase (+)
catalase (+)
growth in 7.5% NaCl
ferment mannitol
Staph aureus infections
furuncles (boils)
bullous impetigo
wounds
bacteremia
Staph aureus intoxications
scalded skin syndrome
toxic shock syndrome
gastritis/food poisoning (enterotoxin, 1-5 hrs after eating)
Staph aureus info
most beta lactamase (+)
many MRSA
Staph aureus resistance/sensitivity
most (R) to penicillin due to plasmid mediated B lactamase
if MRSA, vancomycin is drug of choice
Staph aureus BAP
soft, opaque, regular colonies
some beta hemolytic
some have pale golden color
Coagulase (-) Staph
opportunist in immunocompromised hosts and patients with prosthetic valves and devices
Staph species
gram positive clusters
Strep species
gram positive pairs and chains
Staph epidermidis info
methicillin (R)
sensitive to novobiocin
Staph saprophyticus infections
UTI in young women
Staph saprophyticus info
(R) to novobiocin
Strep pyogenes info
beta hemolytic
bacitracin (S)
optochin (R)
PYR (+)
Strep pyogenes
group A strep
Strep pyogenes infections
pharyngitis wounds scarlet fever - strawberry tongue impetigo sequelae-rheumatic fever necrotizing fasciitis
Strep agalactiae
group B strep
Strep agalactiae info
beta/gamma hemolytic
hippurate (+)
CAMP (+)
Strep agalactiae infections
neonatal septicemia and meningitis
UTI (CAMP +)
Enterococcus info
alpha/beta/gamma hemolytic
bile esculin (+)
6.5% NaCl (+)
PYR (+)
Enterococcus infections
UTI endocarditis (treat with aminoglycoside + penicillin)
non-Enterococcus
group D
non-Enterococcus group D strep
alpha/gamma hemolysis bile Esculin (+)
Strep bovis
associated with colon cancer
Strep pneumoniae
alpha hemolytic (water drop crater colonies) optochin (S) bile solubility (+)
Strep viridans
alpha/gamma hemolytic
Bacteremia
bacterial infections in bloodstream
Meningitis
bacterial infection of outer membrane of brain
Vanco intermediate staph aureus
overproduction of cell wall target
Vanco resistance staph aureus
alteration of cell wall target