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Anaerobic Bacteria Flashcards

1
Q

Obligate aerobe

A

only aerobic growth - oxygen required

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2
Q

Facultative anaerobe

A

aerobic and anaerobic growth - greater growth with oxygen present

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3
Q

Obligate anaerobe

A

only anaerobic growth - cannot grow when oxygen is present (toxic)

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4
Q

Aerotolerant anaerobe

A

anaerobic growth - growth continues with oxygen present

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5
Q

Microaerophilic

A

aerobic growth - oxygen required in low concentrations

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6
Q

Capnophilic

A

aerobic growth - CO2 required in high concentration

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7
Q

BBE agar

A

enriched, selective, differential for B fragilis group

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8
Q

KVL agar

A

enriched, selective, differential - prevents growth facultative gram nneg and gram pos
larked blood enhances pigment formation

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9
Q

CNA agar

A

inhibits gram neg and used to grow most gram pos and facultative

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10
Q

Egg yolk agar

A

detect proteolytic enzymes produced by clostridium

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11
Q

lecithinase

A

opaque zone around colony

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12
Q

lipase

A

blue green sheen on surface around colony

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13
Q

Bacteroides fragilis group ID

A 
gram (-) rod
coccobacilli
20% bile (+)
bile esculin (+)
vancomycin (R)
kanamycin (R)
colistin (R)
growth on KVL agar
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14
Q

Prevotella ID

A 
gram (-) rod
pigmented (black)
brick red under UV light
vancomycin (R)
kanamycin (R)
growth on KVL agar
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15
Q

Porphyromonas ID

A 
gram (-) rod
pigmented (black)
brick red under UV light
colistin (R)
indole (+)
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16
Q

Fusobacterium ID

A

gram (-) rod
vancomycin (R)
indole (+)

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17
Q

Fusobacterium nucleatum

A

gram (-) rod

causes serious pulmonary infections - most common

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18
Q

Fusobacterium necrophorum

A

gram (-) rod

lung and liver abscesses - more serious

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19
Q

Fusobacterium infection

A

Lemierres syndrome

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20
Q

Veillonella ID

A

gram (-) cocci
red fluorescence
nitrate (+)
mouth/urogenital infection

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21
Q

Acidaminococcus ID

A

gram (-) cocci

GI tract infection

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22
Q

Megasphaera ID

A

gram (-) cocci
GI tract infection
glucose (+)

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23
Q

Finegoldia magna ID

A

gram (+) cocci

Skin/soft tissue/bone/joint infections

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24
Q

Peptostreptococcus ID

A

gram (+) cocci

abdominal and female genital tract infections

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25
Anaerococcus ID
gram (+) cocci | abdominal and female genital tract infections
26
Parvimonas micra ID
gram (+) cocci | oral pathogen
27
anaerobic gram (+) cocci
olive green pigment colonies that become black inhibition by SPS adhesion factors, biofilm production (virulence factors)
28
Clostridium species
(R) to disinfection processes
29
Clostridium perfringens infections
gas gangrene post abortion sepsis food poisoning
30
Clostridium perfringens virulence factors
enterotoxin and exotoxin that can cause severe tissue damage (histotoxin)
31
Clostridium tetani virulence factor
tetanospasmin - neurotoxin that effects spinal cord resulting in involuntary muscle contractions (begin with lock jaw and progress to backward arching of back muscles)
32
Clostridium tetani treatment
DPT - diphtheria, pertussis, and tetanus trivalent vaccine
33
Clostridium botulinum infections
food botulism infant botulism wound botulism iatrogenic botulism
34
food botulism
home canned foods containing spores
35
infant botulism
ingest spores then grow in intestines and produce toxin
36
wound botulism
contaminated with spores - increase with IV drug abuse
37
iatrogenic botulism
toxin therapy for excessive muscle activity or cosmetic use (overdose)
38
Clostridium botulinum virulence factor
botulism toxin - neurotoxin that binds to the synapse of nerve fibers, resulting in acute (flaccid) paralysis and death
39
Clostridium difficile info
high carriage rate in intestines of patients who have received broad spectrum antimicrobial agents and have eliminated normal intestinal flora
40
Clostridium difficile virulence factors
enterotoxin A and cytotoxin B
41
Clostridium difficile diagnosis
growing toxin production bacteria from stool sample detecting toxin in stool using cell monolayers detecting toxin in stool using immunoassays detecting glutamate dehydrogenase antigen molecular detection of the toxin gene
42
Clostridium septicum infection
suggests colon cancer when found in blood cultures | occasionally cause of gas gangrene
43
Clostridium septicum virulence factor
histotoxin
44
Clostridium perfringens ID
double zone beta hemolysis lecithinase (+) subterminal spores nagler test (+)
45
Clostridium tetani ID
round terminal spores resembling drumsticks
46
Clostridium botulinum ID
lecithinase (+) lipase (+) oval/subterminal spores resembling tennis rackets
47
Clostridium difficile ID
CCFA agar - fluoresce under UV light | oval/subterminal spores
48
Clostridium septicum
swarming medusa head
49
Actinomyces infection
causes lumpy jaw, abdominal and chest infections and pelvic infections in women with intrauterine devices
50
Actinomyces virulence factor
fimbriae
51
Cutibacterium acnes infection
plays a role in acne and is recovered from infections of prosthetic devices - also can cause corneal ulcers after cataract surgery opportunistic pathogen
52
Cutibacterium acnes virulence factor
production of enzymes that can cause tissue damage
53
Mobiluncus infection
associated with bacterial vaginitis, pelvic inflammatory disease and abdominal infections
54
Mobiluncus virulence factor
ability to adhere to epithelial cells
55
Lactobacillus info
helps to maintain acidic environment in vagina is population decreases, risk of BV increases rarely pathogenic, used as probiotic
56
Bifidobacterium info
mostly nonpathogenic - probiotic
57
Eubacterium
mostly nonpathogenic
58
Actinomyces ID
filamentous branching rods | molar tooth
59
Cutibacterium ID
anaerobic diphtheroid catalase (+) indole (+)
60
Mobiluncus ID
``` stains gram (-) but has a cell wall structure of gram (+) curved rod ```
61
Lactobacillus ID
aerotolerant | alpha hemolytic on blood agar
62
Bifidobacterium ID
bifurcated rods | indole (+)

Microbiology (37 decks)

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