Genetic Engineering

Question 1

Genetic engineering

Answer 1

The manipulation of genes
-not a natural process
-most common vector used is a bacterial plasmid

Question 2

Process 1.

Answer 2

Isolation of dna from a cell and identifying a gene

Question 3

Process 2.

Answer 3

Cutting of a gene from human chromosomes and the plasmid dna using the same restriction enzyme

Question 4

Process 3.

Answer 4

Ligation of the target gene from the host and plasmid dna using dna ligase
Transformation is the uptake of dna into a cell

Question 5

Process 4.

Answer 5

The base sequence changes order in the host

Question 6

Process 5.

Answer 6

Gene expression - production of a protein

Question 7

Uses of genetic engineering

Answer 7

Plants - seedless fruit
Animals - lactose free milk
Microorganisms- using bacteria to make insulin for humans

Question 8

Ethics of genetic engineering

Answer 8

Animals might suffer as a result of being genetically modified
The use of GMOS as a food source

Question 9

Altered DNA

Answer 9

Is called recombinant dna
Organism with altered dna is known as a genetically modified organism (GMO)