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PMLS 2 > (F) Lesson 5: Laboratory Assistant/Laboratory Waste Management Plan > Flashcards

(F) Lesson 5: Laboratory Assistant/Laboratory Waste Management Plan Flashcards

1
Q

T or F: Laboratory assistant have job assignments that vary from one institution to another

A

True

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2
Q

A laboratory assistant may be assigned into which two roles?

A

A. Receptionist in the reception area
B. Laboratory aide in testing areas

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3
Q

T or F: Laboratory glasswares should not only be clean physically but also chemically.

A

True

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4
Q

What is the common two-way rinse of washing laboratory glassware?

A

With tap and distilled/deionized water

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5
Q

How do you commonly dry laboratory glassware?

A

Air dry or with drying oven

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6
Q

In removing grease, you need to boil the glassware in which weak solutions?

A

Sodium carbonate, acetone, or any fat solution

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7
Q

For greasy stopcock in laboratory equipment, in which solution do you soak it and for how long?

A

In a warm decahydronaphthalene for 2 hours

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8
Q

In removing grease, what do you use to rinse it and for how long?

A

With acetone or fuming sulfuric acid for 30 mins

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9
Q

In rinsing, how many times do you fill with water, shake, and empty each test tube, graduated cylinder, and/or flask?

A

6 times

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10
Q

These are machines/a type of technology that can be used for cleansing pipets

A

Pipet washers

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11
Q

T or F: In washing pipets, place pipets with tips up in a cylinder or tall jar of water.

A

False (tips down)

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12
Q

If the pipet is dirty, where do you soak it?

A

In a jar of chromic acid cleaning solution

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13
Q

How long do you soak in distilled water the pipet after rinsing with tap water?

A

At least 1 hour

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14
Q

In washing, the culture tubes are autoclaved for how long?

A

30 mins

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15
Q

In washing, the culture tubes are autoclaved in which temperature and pressure setting?

A

121 deg C and 15 psi

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16
Q

What are used to sterilize Petri dishes and culture bottles?

A

Autoclave or a dry-air sterilizer

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17
Q

In sterilizing serology tubes, what do you add in the bucket before boiling?

A

1% - 2% soap or detergent

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18
Q

In sterilizing serology tubes, how long do you boil them?

A

30 mins

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19
Q

What is the drying temperature for serology tubes and culture media?

A

less than 140 deg C

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20
Q

Where do you hang serology tubes to dry?

A

Wooden pegs

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21
Q

T or F: Acidic liquids should not be stored in flasks because the stoppers or stopcocks may stick

A

False (alkaline)

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22
Q

Where do you document the cleaning and maintenance activities in the laboratory?

A

Laboratory logbook

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23
Q

T or F: The laboratory exit floor plan should have three clear passageways.

A

False (two)

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24
Q

T or F: When designing the location of safety showers, eyewashes, fire extinguishers, etc, aesthetic value should be considered

A

False (must be strategically placed)

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25
Q

T or F: Fire extinguisher should be placed near the door

A

True

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26
Q

What should be maintained and filled with water to avoid sewer gases from escaping?

A

Sink traps and floor drains

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27
Q

What should be placed away from any edges?

A

Bench apparatus

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28
Q

T or F: Cleaning reagents must be prepared at least twice a week

A

False (daily)

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29
Q

T or F: Cleaning reagents is safer and more efficient to store in diluted form

A

False (should not)

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30
Q

What are the labels put in preparing cleaning reagents?

A

Name and date of preparation

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31
Q

Disinfectant used for daily cleaning

A

1 % sodium hypochlorite solution

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32
Q

To prepare 1 % sodium hypochlorite solution, add how many mL of sodium hypochlorite solution to how many mL of water?

A

10 mL of sodium hypochlorite to 1000mL of water (10:1000)

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33
Q

To produce 1 liter of 70% ethanol, how many mL of absolute ethanol shall be added with how many mL of distilled water?

A

700mL of absolute ethanol with 300mL of distilled water (700:300)

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34
Q

1 liter of 5% Lysol is produced by adding how many mL of 12% Lysol to how many mL\ of distilled water?

A

450 mL of 12% Lysol to 550 mL of distilled water

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35
Q

In daily cleaning procedure, which disinfectant is poured and spread on the work surface with paper towel?

A

5% Lysol

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36
Q

What two-way disinfection is used in weekly cleaning of the work area?

A

Soap solution anf 5% Lysol

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37
Q

In weekly cleaning of the work area, which disinfectant do you use when you clean the ultrasonic water bath?

A

Hypochlorite (bleach) solution

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38
Q

In weekly cleaning of the work area, which disinfectant do you use when you clean the racks and tubes?

A

5% Lysol

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39
Q

In weekly cleaning of the work area, which disinfectant do you use when you clean the racks for molecular testing?

A

1% sodium hypochlorite (bleach solution)

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40
Q

Recite the waste management hierarchy pyramid from most preferable to least preferable

A

Reduce
Reuse
Recycle
Recover
Dispose

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41
Q

Waste management hierarchy; Minimizing the amount of waste produced

A

Reduce

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42
Q

Waste management hierarchy; using products repeatedly

A

Reuse

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43
Q

Waste management hierarchy; using materials to make new products

A

Recycle

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44
Q

Waste management hierarchy; obtaining energy from waste

A

Recovery

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45
Q

Waste management hierarchy; landfill and incineration without energy recovery

A

Disposal

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46
Q

T or F: The waste management plan should not only focus on minimizing the potential hazardous risk but also consider minimizing the impact of the disposal of waste materials on the environment

A

True

47
Q

Tier of strategic hierarchy; pollution prevention and source reduction

A

Green chemistry

48
Q

Tier of strategic hierarchy; used to treat unwanted materials, surplus chemicals, and hazards

A

Reuse, redistribute, and reduce

49
Q

Tier of strategic hierarchy; recycling chemicals and recover energy from waste

A

Recycle

50
Q

Tier of strategic hierarchy; refers to incineration, land disposal, and other treatment methods

A

Environmental fate of the waste

51
Q

Refers to the primary consideration of waste disposal

A

Safety

52
Q

Refers to the temporary storage for waste in the laboratory

A

Satellite accumulation area

53
Q

T or F: Waste materials have different categories and corresponding safety precautions and follow an appropriate disposal method

A

True

54
Q

These are trays used for spills and leakages from any primary container

A

Secondary containment devices

55
Q

Waste materials in the satellite accumulation should not be kept for how long?

A

1 year

56
Q

Waste exceeding how many gallons should be managed within 3 days?

A

55-gal

57
Q

Waste exceeding the 55-gal (or 1-qt) limit should be managed accordingly within how many days?

A

3 days

58
Q

T or F: It is fine to mix incompatible waste

A

False

59
Q

T or F: It’s okay to use the same funnel for containers with incompatible waste materials

A

False

60
Q

T or F: Separately collect aqueous wastes from organic solvent wastes.

A

True

61
Q

Glass is not used as a waste container if there is a presence of which danger?

A

Danger of freezing (can expand and break the glass)

62
Q

T or F: In disposal of empty containers, rinsing properly is advised if necessary

A

True

63
Q

Category of Health Care Waste; Not in contact with infectious agents, hazardous chemicals, radioactive materials

A

Non-hazardous or general waste

64
Q

Category of Health Care Waste; refer to any specimen from a patient

A

Infectious wastes

65
Q

Category of Health Care Waste; discarded needles used to give shots or draw blood and discarded lancets

A

Sharps

66
Q

Category of Health Care Waste; Tissue sections and body material derived from biopsies or surgical procedures

A

Pathological waste

67
Q

Category of Health Care Waste; Recognizable body parts usually from amputation procedures

A

Anatomical waste

68
Q

Category of Health Care Waste; hazardous and non-hazardous substances from the laboratory

A

Chemical waste

69
Q

Hazardous chemicals that are health and environment hazards

A

Toxic

70
Q

Hazardous chemicals that are strong acids and bases

A

Corrosive

71
Q

pH range of corrosive chemicals

A

less than 2.0 and/or greater than 12.0

72
Q

Hazardous chemicals with flash point below 600 C

A

Flammable

73
Q

Hazardous chemicals that are explosive with water

A

Reactive

74
Q

Category of Health Care Waste; refers to containers that may explode

A

PRessurized containers

75
Q

Category of Health Care Waste; radioactive or cytotoxic materials such as excreta of patients who underwent radionuclide procedures

A

Radioactive waste

76
Q

Color of bin/liner of infectious waste

A

Yellow

77
Q

Color of bin/liner of pathological and anatomical waste

A

Yellow

78
Q

Color of bin/liner of sharps and pressurized containers

A

Red

79
Q

Color of bin/liner of chemical waste

A

Yellow (with black band)

80
Q

Color of bin/liner of pharmaceutical waste

A

Yellow (with black band)

81
Q

Color of bin/liner of radioactive waste

A

Orange

82
Q

Color of bin/liner of general waste (nonbiodegradable)

A

Black

83
Q

Color of bin/liner of general waste (biodegradable)

A

Green

84
Q

Refers to the process of changing the biological and chemical characteristics of waste to minimize its potential to cause harm

A

Treatment

85
Q

Processes that refer to relying on heat to destroy pathogens

A

Thermal processes

86
Q

Refers to thermal decomposition of health care
wastes with absence of molecular oxygen

A

Pyrolysis

87
Q

Where are residues of pyrolysis disposed?

A

Landfill

88
Q

Refers to the use of steam sterilization with indicators

A

Autoclave

89
Q

Heat and pressure of autoclave, and for how long

A

121 deg C, 15 psi for 15 - 30 mins

90
Q

Refers to microorganisms being destroyed by heat which coagulates and denatures enzymes and proteins

A

Microwave

91
Q

Temperature requirement for microwave and for how long

A

100 deg C for 30 mins

92
Q

Solutions used in Chemical Disinfection treatment

A

Aldehydes, chlorine compounds, phenolic compounds such as Lysol

93
Q

Refers to using enzyme mixture to decontaminate health care waste

A

Biological process

94
Q

Kind of waste treatment that refers to breaking down the DNA molecules of the microorganism

A

Radiation Technology

95
Q

Refers to filling up of containers with waste, adding immobilizing material, then sealing the container

A

Encapsulation

96
Q

Which type of waste is commonly treated by encapsulation and inertization?

A

Pharmaceutical waste

97
Q

Refers to a variant of encapsulation; pharmaceuticals ground then mix with water, cement and lime to form a homogenous paste

A

Inertization

98
Q

Refers to an engineered site designed to keep waste isolated from the environment

A

Sanitary Landfill

99
Q

Refers to where treated infectious wastes, sharps, pathological and anatomical wastes are disposed; health care facilities in remote areas

A

Safe burial

100
Q

Refers to where sharps and syringes can be disposed

A

Septic or concrete vaults

101
Q

Where are infectious wastes disposed?

A

Landfill

102
Q

How is pathological waste disposed?

A

By burying

103
Q

How is chemical waste disposed?

A

By recycling and draining down the sink (wtf)

104
Q

How are radioactive wastes treated?

A

Waiting to decay

105
Q

How are radioactive wastes disposed?

A

By the supplier

106
Q

Where are aerosol and pressurized containers disposed?

A

By landfill or disposal by supplier

107
Q

Where are sharps wastes disposed?

A

Landfill and by burying

108
Q

Where are aerosol and pressurized containers disposed of?

A

By landfill or disposal by the supplier

109
Q

How are expired pharmaceutical wastes disposed of?

A

By the supplier

110
Q

You should wait how long to dispose serum/CSF/body fluid specimen?

A

48 hours

111
Q

You should wait how long to dispose urine specimens?

A

24 hours

112
Q

You should wait how long to dispose blood/fluid smears?

A

7 days

113
Q

You should wait how long to dispose microbiology-stained slides?

A

7 days

114
Q

You should wait how long to dispose surgical pathology slides?

A

10 years

PMLS 2 (16 decks)

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