(F) Lesson 5: Laboratory Assistant/Laboratory Waste Management Plan Flashcards
T or F: Laboratory assistant have job assignments that vary from one institution to another
True
A laboratory assistant may be assigned into which two roles?
A. Receptionist in the reception area
B. Laboratory aide in testing areas
T or F: Laboratory glasswares should not only be clean physically but also chemically.
True
What is the common two-way rinse of washing laboratory glassware?
With tap and distilled/deionized water
How do you commonly dry laboratory glassware?
Air dry or with drying oven
In removing grease, you need to boil the glassware in which weak solutions?
Sodium carbonate, acetone, or any fat solution
For greasy stopcock in laboratory equipment, in which solution do you soak it and for how long?
In a warm decahydronaphthalene for 2 hours
In removing grease, what do you use to rinse it and for how long?
With acetone or fuming sulfuric acid for 30 mins
In rinsing, how many times do you fill with water, shake, and empty each test tube, graduated cylinder, and/or flask?
6 times
These are machines/a type of technology that can be used for cleansing pipets
Pipet washers
T or F: In washing pipets, place pipets with tips up in a cylinder or tall jar of water.
False (tips down)
If the pipet is dirty, where do you soak it?
In a jar of chromic acid cleaning solution
How long do you soak in distilled water the pipet after rinsing with tap water?
At least 1 hour
In washing, the culture tubes are autoclaved for how long?
30 mins
In washing, the culture tubes are autoclaved in which temperature and pressure setting?
121 deg C and 15 psi
What are used to sterilize Petri dishes and culture bottles?
Autoclave or a dry-air sterilizer
In sterilizing serology tubes, what do you add in the bucket before boiling?
1% - 2% soap or detergent
In sterilizing serology tubes, how long do you boil them?
30 mins
What is the drying temperature for serology tubes and culture media?
less than 140 deg C
Where do you hang serology tubes to dry?
Wooden pegs
T or F: Acidic liquids should not be stored in flasks because the stoppers or stopcocks may stick
False (alkaline)
Where do you document the cleaning and maintenance activities in the laboratory?
Laboratory logbook
T or F: The laboratory exit floor plan should have three clear passageways.
False (two)
T or F: When designing the location of safety showers, eyewashes, fire extinguishers, etc, aesthetic value should be considered
False (must be strategically placed)
T or F: Fire extinguisher should be placed near the door
True
What should be maintained and filled with water to avoid sewer gases from escaping?
Sink traps and floor drains
What should be placed away from any edges?
Bench apparatus
T or F: Cleaning reagents must be prepared at least twice a week
False (daily)
T or F: Cleaning reagents is safer and more efficient to store in diluted form
False (should not)
What are the labels put in preparing cleaning reagents?
Name and date of preparation
Disinfectant used for daily cleaning
1 % sodium hypochlorite solution
To prepare 1 % sodium hypochlorite solution, add how many mL of sodium hypochlorite solution to how many mL of water?
10 mL of sodium hypochlorite to 1000mL of water (10:1000)
To produce 1 liter of 70% ethanol, how many mL of absolute ethanol shall be added with how many mL of distilled water?
700mL of absolute ethanol with 300mL of distilled water (700:300)
1 liter of 5% Lysol is produced by adding how many mL of 12% Lysol to how many mL\ of distilled water?
450 mL of 12% Lysol to 550 mL of distilled water
In daily cleaning procedure, which disinfectant is poured and spread on the work surface with paper towel?
5% Lysol
What two-way disinfection is used in weekly cleaning of the work area?
Soap solution anf 5% Lysol
In weekly cleaning of the work area, which disinfectant do you use when you clean the ultrasonic water bath?
Hypochlorite (bleach) solution
In weekly cleaning of the work area, which disinfectant do you use when you clean the racks and tubes?
5% Lysol
In weekly cleaning of the work area, which disinfectant do you use when you clean the racks for molecular testing?
1% sodium hypochlorite (bleach solution)
Recite the waste management hierarchy pyramid from most preferable to least preferable
Reduce
Reuse
Recycle
Recover
Dispose
Waste management hierarchy; Minimizing the amount of waste produced
Reduce
Waste management hierarchy; using products repeatedly
Reuse
Waste management hierarchy; using materials to make new products
Recycle
Waste management hierarchy; obtaining energy from waste
Recovery
Waste management hierarchy; landfill and incineration without energy recovery
Disposal
T or F: The waste management plan should not only focus on minimizing the potential hazardous risk but also consider minimizing the impact of the disposal of waste materials on the environment
True
Tier of strategic hierarchy; pollution prevention and source reduction
Green chemistry
Tier of strategic hierarchy; used to treat unwanted materials, surplus chemicals, and hazards
Reuse, redistribute, and reduce
Tier of strategic hierarchy; recycling chemicals and recover energy from waste
Recycle
Tier of strategic hierarchy; refers to incineration, land disposal, and other treatment methods
Environmental fate of the waste
Refers to the primary consideration of waste disposal
Safety
Refers to the temporary storage for waste in the laboratory
Satellite accumulation area
T or F: Waste materials have different categories and corresponding safety precautions and follow an appropriate disposal method
True
These are trays used for spills and leakages from any primary container
Secondary containment devices
Waste materials in the satellite accumulation should not be kept for how long?
1 year
Waste exceeding how many gallons should be managed within 3 days?
55-gal
Waste exceeding the 55-gal (or 1-qt) limit should be managed accordingly within how many days?
3 days
T or F: It is fine to mix incompatible waste
False
T or F: It’s okay to use the same funnel for containers with incompatible waste materials
False
T or F: Separately collect aqueous wastes from organic solvent wastes.
True
Glass is not used as a waste container if there is a presence of which danger?
Danger of freezing (can expand and break the glass)
T or F: In disposal of empty containers, rinsing properly is advised if necessary
True
Category of Health Care Waste; Not in contact with infectious agents, hazardous chemicals, radioactive materials
Non-hazardous or general waste
Category of Health Care Waste; refer to any specimen from a patient
Infectious wastes
Category of Health Care Waste; discarded needles used to give shots or draw blood and discarded lancets
Sharps
Category of Health Care Waste; Tissue sections and body material derived from biopsies or surgical procedures
Pathological waste
Category of Health Care Waste; Recognizable body parts usually from amputation procedures
Anatomical waste
Category of Health Care Waste; hazardous and non-hazardous substances from the laboratory
Chemical waste
Hazardous chemicals that are health and environment hazards
Toxic
Hazardous chemicals that are strong acids and bases
Corrosive
pH range of corrosive chemicals
less than 2.0 and/or greater than 12.0
Hazardous chemicals with flash point below 600 C
Flammable
Hazardous chemicals that are explosive with water
Reactive
Category of Health Care Waste; refers to containers that may explode
PRessurized containers
Category of Health Care Waste; radioactive or cytotoxic materials such as excreta of patients who underwent radionuclide procedures
Radioactive waste
Color of bin/liner of infectious waste
Yellow
Color of bin/liner of pathological and anatomical waste
Yellow
Color of bin/liner of sharps and pressurized containers
Red
Color of bin/liner of chemical waste
Yellow (with black band)
Color of bin/liner of pharmaceutical waste
Yellow (with black band)
Color of bin/liner of radioactive waste
Orange
Color of bin/liner of general waste (nonbiodegradable)
Black
Color of bin/liner of general waste (biodegradable)
Green
Refers to the process of changing the biological and chemical characteristics of waste to minimize its potential to cause harm
Treatment
Processes that refer to relying on heat to destroy pathogens
Thermal processes
Refers to thermal decomposition of health care
wastes with absence of molecular oxygen
Pyrolysis
Where are residues of pyrolysis disposed?
Landfill
Refers to the use of steam sterilization with indicators
Autoclave
Heat and pressure of autoclave, and for how long
121 deg C, 15 psi for 15 - 30 mins
Refers to microorganisms being destroyed by heat which coagulates and denatures enzymes and proteins
Microwave
Temperature requirement for microwave and for how long
100 deg C for 30 mins
Solutions used in Chemical Disinfection treatment
Aldehydes, chlorine compounds, phenolic compounds such as Lysol
Refers to using enzyme mixture to decontaminate health care waste
Biological process
Kind of waste treatment that refers to breaking down the DNA molecules of the microorganism
Radiation Technology
Refers to filling up of containers with waste, adding immobilizing material, then sealing the container
Encapsulation
Which type of waste is commonly treated by encapsulation and inertization?
Pharmaceutical waste
Refers to a variant of encapsulation; pharmaceuticals ground then mix with water, cement and lime to form a homogenous paste
Inertization
Refers to an engineered site designed to keep waste isolated from the environment
Sanitary Landfill
Refers to where treated infectious wastes, sharps, pathological and anatomical wastes are disposed; health care facilities in remote areas
Safe burial
Refers to where sharps and syringes can be disposed
Septic or concrete vaults
Where are infectious wastes disposed?
Landfill
How is pathological waste disposed?
By burying
How is chemical waste disposed?
By recycling and draining down the sink (wtf)
How are radioactive wastes treated?
Waiting to decay
How are radioactive wastes disposed?
By the supplier
Where are aerosol and pressurized containers disposed?
By landfill or disposal by supplier
Where are sharps wastes disposed?
Landfill and by burying
Where are aerosol and pressurized containers disposed of?
By landfill or disposal by the supplier
How are expired pharmaceutical wastes disposed of?
By the supplier
You should wait how long to dispose serum/CSF/body fluid specimen?
48 hours
You should wait how long to dispose urine specimens?
24 hours
You should wait how long to dispose blood/fluid smears?
7 days
You should wait how long to dispose microbiology-stained slides?
7 days
You should wait how long to dispose surgical pathology slides?
10 years
