(F) Lesson 1: Non-Blood Specimen Handling and Processing Flashcards
Liquid or semi-liquid substances produced by the body and are found within various organs and body spaces
Non-blood specimens
T or F: Most non-blood specimens can be collected by a phlebotomist
False (most are only for physicians)
When a phlebotomist gives instructions regarding specimen collection, what 2 types of instruction should be given?
Oral and written (with illustrations)
T or F: You may verify proper labelling after accepting a specimen for transport
False (before accepting)
T or F: Initials of the phlebotomist are needed when labelling non-blood specimens
False
Where should the label be pasted on the container?
Body; not the lid (risk for interchanging samples as lids are removed during testing)
T or F: Phlebotomists must treat all body substances as potentially infectious
True
What is the most frequently analyzed non-blood body fluid?
Urine
How many hours before a urine sample deteriorates?
2 hours
This is the most commonly requested urine test
Routine urinalysis
T or F: Routine urinalysis is not part of a physical exam
False
This type of analysis for urine samples utilizes observation with the naked eye
Physical or macroscopic observation
This macroscopic observation tells us about the degree of hydration
Color
A darker urine color indicates what?
Concentrated sample due to dehydration
This macroscopic observation is aka transparency
Clarity
A turbid urine sample indicates the presence of what?
Bacteria, WBCs, etc.
What 2 methods measure specific gravity?
Urinometry and Refractory
What 2 macroscopic parameters measure urine concentration
Specific gravity and osmolality
High specific gravity means what in terms of concentration?
Concentrated
T or F: Odor, volume, and osmolality are considered routine urinalysis procedures
False
This macroscopic parameter is only measured if the tests are for quantitative analysis
Volume
This macroscopic parameter can also measure the degree of hydration (other than color) of the specimen as it is more preferred yet more expensive
Osmolality
T or F: Urine should be transported at room temperature
False (with ice)
T or F: Outpatient urine samples are handled by nurses
False (nurses handle inpatient samples while technicians handle the outpatient samples)
T or F: Urinalysis can be ordered during hospitalization
True
Physical or macroscopic observation is also known as?
Gross analysis
A plastic reagent strip is also known as?
Dipstick
This type of urine analysis method utilizes reagent strips to look for substances such as bacteria, bilirubin, blood glucose, ketones, leukocytes, nitrite, protein, and urobilinogen
Chemical analysis
This urine analyte is used to detect the presence of WBCs
Leukocytes
This urine analyte is used to test for bacteria
Nitrite
This analysis method in urinalysis looks for cells, crystals, and microorganisms
Microscopic analysis
T or F: Urine is centrifuged for use in microscopic analysis
True
For how long is urine centrifuged?
5 minutes
T or F: In microscopic analysis for urine, the supernatant is used while the sediments are discarded
False (supernatant is discarded while sediments are used)
WBCs in urine indicate what disorder?
Pyuria
RBCs in urine indicate what disorder?
Hematuria
WBCs and bacteria in urine indicate what condition?
UTI
How many mL of urine is centrifuged for microscopic analysis?
10-15mL
T or F: Microscopic urinalysis examines specimen under a microscope from HPO to LPO
False (from low power objective to high power objective)
T or F: Urine containers for culture and sensitivity testing should be sterile
True
T or F: Urine in containers should be kept at room temperature
True
Urine must be protected from light for up to how many hours?
2 hours
T or F: Specimens held for longer should be refrigerated as part of a preservation protocol
True
What tube must urine be collected in for bilirubin testing?
Amber tube
This urine test confirms UTIs and checks for bacterial growth
Urine culture and sensitivity (CS)
T or F: A bacterial count of over 10,000 cfu/mL means that there is significant urinary tract infection
False (> 100,000 cfu/mL)
CFU stands for?
Colony forming units
Urine for CS is measured using what nutrient medium tool?
A loop
What is the incubation period of urine swabs for CS?
18-24 hours
What is the optimal temperature for bacterial growth in urine CS?
35°-37°C
If a microorganism is identified, this test is performed to determine which antibiotics will be effective against it
Sensitivity or antibiotic susceptibility test
T or F: Culture and sensitivity utilizes a swab instead of a loop
False (a loop is used for CS while a swab is used for sensitivity testing)
T or F: The larger the zone of inhibition, the less effective the antibiotic
False (larger ZOI = more effective)
This test detects cancer, cytomegaloviruses, and other viral and inflammatory diseases of the urinary tract
Urine cytology studies
T or F: Urine cytology studies use smears not containing cell sediments from the urinary tract
False (cell-containing)
This urine stain checks for the presence of abnormal cells under a microscope
Papanicolaou (PAP) stain
How much percent of an equal volume of alcohol should be used when preserving pap smears?
50%
These are used by athletes to enhance performance as these are also detected under urine drug screening
Anabolic steroids
T or F: Urine drug screening procedures are not random
False (are random)
These documents are needed when performing urine drug screening
Chain of custody and control forms
T or F: If the SG of urine is less than 1.003, ask for another sample
False (more than)
T or F: The urinals in drug screening are waterless and the sinks are separated from the toilet area
True
What temperatures indicate that the urine is freshly collected?
32.5°-37°C
T or F: Concentrated urine can give false negative results
False (diluted)
T or F: Dilute urine specimen has increased creatinine
False (decreased creatinine)
These 2 chemicals are ALWAYS paired together when testing urine
Glucose and Ketone
High glucose in BOTH blood and urine indicate what condition?
Diabetes mellitus
Normal blood glucose but high glucose levels in urine indicate what condition?
Renal disease
T or F: Traces of glucose can be found in urine once glucose levels lie within the renal threshold which is 140-160mg/dL
False (must exceed the renal threshold in order to detect glucose in urine; passing the threshold means the kidneys can no longer absorb glucose and therefore expel it in the urine)
Ketones found in urine indicate what condition?
Diabetic ketoacidosis
T or F: Ketones are created when the body breaks down fat for energy because one’s diet is sufficient in carbohydrates
False (deficient)
T or F: Ketones are created when the body breaks down fat for energy because it does not metabolize glucose properly
True
During ketoacidosis, what chemical is absent which triggers the blockage of glucose?
Insulin (therefore glucose is not metabolized properly)
A pregnancy test is able to detect what hormone
Human chorionic gonadotropin hormone
HCG is produced commonly in what part of a pregnant woman’s body?
Developing placenta
How many days after conception is HCG detectable in serum and urine?
8-10 days
What urine specimen is preferred for taking a pregnancy test to avoid false negatives?
First morning specimen
T or F: HCG only appears in pregnant women as this can only be found in a placenta
False (can also appear in the urine of male patients with certain types of cancer and trophoblastic diseases)
This urine specimen is collected at any time and is most commonly analyzed
Random
This urine sample is collected in the morning after 8 hours of sleep and is the most concentrated sample
First morning/8-hour specimen
This urine specimen is the most recommended for routine urinalysis and pregnancy tests due to its high specific gravity
First morning/8-hour specimen
T or F: In a first morning urine specimen, you void the first sample in the toilet
False (you keep the first void)
This urine specimen involves voiding the second specimen after fasting to be used for glucose monitoring and glucose testing
Fasting (fasting second morning)
T or F: You void the first sample in the toilet for a fasting urine sample because it has remnants of the food taken from the last meal
True
This urine specimen is collected at specific times or collected and pooled throughout a specific time period
Timed
This subtype of timed urine specimen is collected serially at specific times that correspond with the timing of blood collection
Tolerance test specimen
T or F: The interval for tolerance test specimen collection is: fasting, 1 hour, and 2 hours
False (fasting, 1/2 hour, 1 hour, 2 hours)
T or F: Glucose levels before a meal should be higher than 100mg/dL as urine is more concentrated
False (lower than 100mg/dL)
How many mg/dL of glucose is expected after 2 hours of collecting a tolerance test specimen?
140
This subtype of timed urine specimen is collected 2 hours after a meal and tested for glucose to monitor insulin therapy of patients with diabetes mellitus
2-hours postprandial
T or F: When conducting a 2-hour postprandial urine test, the patient must start with an empty bladder after consuming a meal
False (void shortly BEFORE consuming a meal then collect 2 hours later)
T or F: You are to compare glucose results on fasting urine and fasting blood specimen
True
This subtype of timed urine specimen is used for quantitative analysis (volume) and collected and pooled for an entire day
24-hour specimen
T or F: 24-hour specimen can only be collected in a wide-mouthed container provided by the hospital
False (patients may use large clean soda bottles as an alternative)
T or F: There are no preservatives present in the wide-mouthed containers prior to 24-hour urine specimen collection
False (preservatives are added prior to collection)
T or F: 24-hour urine specimens are not to be refrigerated throughout collection period
False (refrigerate)
T or F: You void the first sample into a container for a 24-hour urine specimen
False (void in a toilet as this will not be counted within the 24 hours due to it being the urine that was building up in the body the night before)
T or F: Collect urine before anticipated bowel movement
True