exp 1 Flashcards

1
Q

MAIN GOAL of the experiment

A

isolation of ALKALOIDS form GROUND ARABICA COFFEE BEANS

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2
Q

2 types of extraction used in this experiment

A
  1. solid-liquid extraction
  2. liquid-liquid extraction
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3
Q

Alkaloids are derivatives of?

A

ammonia

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4
Q

Alkaloids are characterized by the presence of ____ in a ____

A

basic nitrogen

heterocyclic ring

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5
Q

Alkaloids usually occur as… (2)

A

Tertiary or secondary amines

due to being derivatives of NH3

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6
Q

EXAMPLES of alkaloids (5)

A
  1. pyrrole
  2. pyridine
  3. pyrrolidine
  4. quinoline
  5. isoquinoline
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7
Q

What makes alkaloids basic?

A

The nag-i-isang LONE PAIR present in Nitrogen in the alkaloid

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8
Q

What is the alkaloid of interest for the experiment?

A

CAFFEINE

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9
Q

Does caffeine have HIGH or LOW polarity?

A

LOW polarity = does not readily dissolve in water at room temperature

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10
Q

Major alkaloid found in tea leaves?

and how much?

A

Caffeine : 3-5% by weight

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11
Q

Once alkaloids is isolated as a solid, what will be its color?

A

white / off-white

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12
Q

How to ascertain if the isolated solid from the extraction procedure?

A

WAGNER’S TEST (a chemical test)

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13
Q

Will caffeine be the only alkaloid will be/possible to be isolated?

A

NO, there are many other alkaloids aside from caffeine

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14
Q

What other alkaloids can be separated from coffee beans?

A

Theobromine, theophylline, trigonelline

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15
Q

Why is it possible to isolate alkaloids from such compounds?

A

Solubility of alkaloids in organic solvents (low polarity) and water

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16
Q

How much CAFFEINE is present in Arabica coffee bean?

A

1.2 - 1.5% by weight

17
Q

What is solubility?

A

Ability of 2 or more to form a HOMOGENOUS mixture spontaneously without a chemical reaction

18
Q

Which interaction is higher:

solute-solvent

or

solute-solute

A

solute-solvent (meaning mas mag ddisolve)

19
Q

Guiding principle of solubility

A

“like dissolves like”

20
Q

Solubility is based on what process?

and what does THIS process require and why?

A

separation of solute molecules from each other to spread amongst solvent molecules

  • requires energy to overcome the intermolecular forces in the solute
21
Q

If alkaloids are not that soluble in water @ room temp, what temperature are they soluble?

A

high temperature -> hot water

22
Q

Examples of ORGANIC SOLVENTS (3)

A
  1. dichloromethane
  2. chloroform
  3. ethyl acetate
23
Q

How can solubility of alkaloids in water be enhanced?

and why can this happen?

A

addition of dilute strong acids (gives H+ to alkaloids)

results to a stronger interaction: ion-dipole forces

wherein: ion = positively charged alkaloids
dipole = polar water

24
Q

What is extraction

A

laboratory technique for transferring a solute from a solid or liquid into a different solvent or phase

25
Q

SOLID-LIQUID EXTRACTION vs LIQUID-LIQUID EXTRACTION

A

SLE: separation of solute from a solid material (coffee bean) by CONTACT WITH low-boiling solvent (water)

LLE: transfer of solute from one liquid component to an EXTRACTING SOLVENT (wherein the solute should have a higher solubility in para makuha ni E.S. yung solute)

26
Q

What extracting solvent will we use in the experiment?

A

ethyl acetate

27
Q

In liquid-liquid extraction, what should you remember?

A

the 2 liquids that you will use should be IMMISCIBLE

28
Q

How does the liquid-liquid extraction exactly work?

A
  • mix the container where you will perform the LLE
  • the solute should become distributed in the two layers
  • a higher amount of solute will go to the layer where it is more soluble in
29
Q

What is the funnel used in liquid-liquid extraction?

A

Separatory funnel

30
Q

What test to determine if what composition is at the bottom layer of the separatory funnel?

A

WATER TEST

  • if nag dissolve: it’s water
  • if di nag dissolve: it’s organic solvent
31
Q

LIQUID-LIQUID EXTRACTION

Aqueous layer VS Organic layer

A

AL: water

OL: organic solvent

32
Q

Purpose of DILUTE STRONG ACID and DILUTE WEAK BASE in this experiment

A

DSA: mixed with water to extract the alkaloids from the coffee bean. thus, alkaloids will become + charged

DWB: used to neutralize the alkaloids

33
Q

In Wagner’s Test, what confirms the presence of alkaloid in the sample?

A

reddish brown precipitate

34
Q

WASTE DISPOSAL

  • Filtrate & Decantate (Liquids)
  • Wagner’s Test
  • Tira-tirang solids
A
  • G704 (non-halogenated org solvents)
  • E501
  • Dissolve with water + E501