EXAM 4: QUIZ- METHODS, ENZYMES, EPHORESIS, PCR, CLONING Flashcards
The purpose of PCR is to create
billions of copies of a segment of DNA.
_____ uses an electrical field to separate DNA fragments based upon their length.
Gel electrophoresis
In DNA fingerprinting the products of PCR are cut by restriction enzymes into unique lengths;
these fragments are then
separated according to the lengths of the fragments.
DNA ligase that is used to seal the piece of foreign DNA into the vector is
naturally occurring in both humans and bacteria and functions in DNA replication.
What structure is used to seal the DNA into an opening created by the restriction enzyme during recombinant DNA technology?
DNA ligase
What is the substance required to cleave the vector DNA during recombinant DNA technology?
restriction enzymes
PCR is often used in forensic analysis since
very small samples are frequently all that are available.
What technique is used to create billions of copies of DNA in short amount of time?
polymerase chain reaction
true or false regarding restriction enzymes: They are used during the manufacture of proteins
false
true or false: RNA E. plasmids are used in the production of recombinant DNA (rDNA)?
false
A method used to determine if the DNA found at a crime scene belongs to a particular individual is short tandem repeat (STR). This method employs
repeated sequences found at multiple sites in the DNA.
what are the 3 uses of DNA fingerprinting
- paternity test
- forensic analysis
- determining a predisposition to cancer
Why can engineered DNA polymerase be used in PCR and not the one that occurs naturally?
It must work on single-stranded DNA at high temperatures and attach to a primer.
A piece of DNA that contains sequences from two or more different organisms is __ DNA.
recombinant
When human DNA is inserted into bacterial plasmid, the resulting bacterium will
produce the products of the gene that has been inserted into the bacteria.
