EXAM 4- METHODS: CELLS Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What are the 3 types of microscopes

A
  • light
  • confocal
  • electron
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what microscope would give you a 3D image

A

scanning electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what microscope would give you a 2D image

A

transmission electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what microscope would you use to look at a X section

A

transmission electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what kind of microscope gives you a picture of the surface of a sample

A

scanning electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is the resolution of a light microscope

A

0.2 um appart

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what are the 2 types of light microscopes

A
  • light

- confocal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what are the 2 lenses in a light compound microscope

A
  • ocular

- objective

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what kind of microscope: reduction of background for high quality images

A

confocal

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q

A confocal microscope collects serial ____ _______ from _____ specimens

A
  • optical sections

- thick

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what is used to visualize molecules in light microscope

A
  • antibodies
  • radioisotopes
  • GFP
  • probe
How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

what does GFP stand for

A

green fluorescence protein

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

what is the 2 problems with GFP

A
  • the sample will start to degrade over time and fade

- only dead samples can be used

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

true or false: the secondary antibody will be the one to permanently stain the sample

A

true

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what kind of microscope: for looking at organelles and molecules

A

electron microscope

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

what are the 2 types of electron microscopes

A
  • scanning

- transmission

17
Q

would you use a scanning or transmission electron microscope to look at structures

A

transmission

18
Q

describe the samples for an electron microscope

A
  • fixed

- very thin

19
Q

what allows surface features to be examined at high resolution by negative staining

A

immunogold electron microscopy metal shadowing

20
Q

separate individual cells from whole tissue

A

cell extraction

21
Q

what does the tissue get treated with in cell extraction

A

protease and other enzymes

22
Q

how are cells ID in cell extraction

A
  • fluorescently- labeled antibodies

- fluorescence- activated cell sorter

23
Q

antibodies tag cell surface markers

A
  • fluorescently- labeled antibodies
24
Q

separates cells according to fluorescence antibody tag

A
  • fluorescence- activated cell sorter
25
Q

what is the purpose of culturing cells

A
  • control environment
  • sterile
  • mimic growing requirements
26
Q

term? embryonic stem cells that can differentiate into anything

A

pluripotent

27
Q

true or false: adult stem cells are limited to what they can become

A

true

28
Q

describe cloned animals lifespan

A

they have a shorter lifespan than the animal they were cloned from

29
Q

what does in situ hybridization determine

A

presence of genes on tissue

30
Q

what is the abbreviation for terminal deoxynucleotidyl transferase dUTP nick end labeling

A

TUNEL

31
Q

what does the TUNEL ASSAY determine

A

locates DNA fragmentation caused by cell death