EXAM 4- METHODS: CELLS Flashcards
What are the 3 types of microscopes
- light
- confocal
- electron
what microscope would give you a 3D image
scanning electron microscope
what microscope would give you a 2D image
transmission electron microscope
what microscope would you use to look at a X section
transmission electron microscope
what kind of microscope gives you a picture of the surface of a sample
scanning electron microscope
what is the resolution of a light microscope
0.2 um appart
what are the 2 types of light microscopes
- light
- confocal
what are the 2 lenses in a light compound microscope
- ocular
- objective
what kind of microscope: reduction of background for high quality images
confocal
A confocal microscope collects serial ____ _______ from _____ specimens
- optical sections
- thick
what is used to visualize molecules in light microscope
- antibodies
- radioisotopes
- GFP
- probe
what does GFP stand for
green fluorescence protein
what is the 2 problems with GFP
- the sample will start to degrade over time and fade
- only dead samples can be used
true or false: the secondary antibody will be the one to permanently stain the sample
true
what kind of microscope: for looking at organelles and molecules
electron microscope
what are the 2 types of electron microscopes
- scanning
- transmission
would you use a scanning or transmission electron microscope to look at structures
transmission
describe the samples for an electron microscope
- fixed
- very thin
what allows surface features to be examined at high resolution by negative staining
immunogold electron microscopy metal shadowing
separate individual cells from whole tissue
cell extraction
what does the tissue get treated with in cell extraction
protease and other enzymes
how are cells ID in cell extraction
- fluorescently- labeled antibodies
- fluorescence- activated cell sorter
antibodies tag cell surface markers
- fluorescently- labeled antibodies
separates cells according to fluorescence antibody tag
- fluorescence- activated cell sorter
what is the purpose of culturing cells
- control environment
- sterile
- mimic growing requirements
term? embryonic stem cells that can differentiate into anything
pluripotent
true or false: adult stem cells are limited to what they can become
true
describe cloned animals lifespan
they have a shorter lifespan than the animal they were cloned from
what does in situ hybridization determine
presence of genes on tissue
what is the abbreviation for terminal deoxynucleotidyl transferase dUTP nick end labeling
TUNEL
what does the TUNEL ASSAY determine
locates DNA fragmentation caused by cell death
