EXAM 4- METHODS: CELLS Flashcards
What are the 3 types of microscopes
- light
- confocal
- electron
what microscope would give you a 3D image
scanning electron microscope
what microscope would give you a 2D image
transmission electron microscope
what microscope would you use to look at a X section
transmission electron microscope
what kind of microscope gives you a picture of the surface of a sample
scanning electron microscope
what is the resolution of a light microscope
0.2 um appart
what are the 2 types of light microscopes
- light
- confocal
what are the 2 lenses in a light compound microscope
- ocular
- objective
what kind of microscope: reduction of background for high quality images
confocal
A confocal microscope collects serial ____ _______ from _____ specimens
- optical sections
- thick
what is used to visualize molecules in light microscope
- antibodies
- radioisotopes
- GFP
- probe
what does GFP stand for
green fluorescence protein
what is the 2 problems with GFP
- the sample will start to degrade over time and fade
- only dead samples can be used
true or false: the secondary antibody will be the one to permanently stain the sample
true
what kind of microscope: for looking at organelles and molecules
electron microscope