EXAM 4- METHODS: NUCLEIC ACIDS

Question 1

process of purifying DNA from samples using physical and chemical methods

Answer 1

extraction of DNA

Question 2

are restriction enzymes endonuclease or exonuclease

Answer 2

endonuclease

Question 3

what cuts double stranded DNA, are bacterial enzymes that protect against viral DNA

Answer 3

restriction enzymes

Question 4

cutting with enzymes

Answer 4

digest DNA

Question 5

technique that examines digested DNA fragment patterns from diff sources since each organism has unique DNA

Answer 5

RFLP/ DNA fingerprinting

Question 6

what does the southern blot ID

Answer 6

specific DNA fragment

Question 7

northern blot

Answer 7

uses probe to find mRNA of a gene

Question 8

copy target gene sequence millions of times starting w/ minimum one molecule

Answer 8

PCR

Question 9

phases of PCR

Answer 9

1. denaturation
2. Hybridization
3. polymerization

Question 10

what technique is used to identify nucleotides

Answer 10

DNA sequencing

Question 11

what is modern DNA sequencing based on

Answer 11

the Sanger method

Question 12

rapid detection of thousands of genes at once

Answer 12

DNA microarray

Question 13

fragment of DNA synthesized and inserted into a plasmid RE and ligation

Answer 13

cassette mutagenesis

Question 14

PCR with primer and mutation site toward on of the ends of the fragment

Answer 14

PCR site directed mutagenesis