EXAM 4- METHODS: PROTEINS Flashcards

You may prefer our related Brainscape-certified flashcards:
1
Q

What process is used to separate proteins

A

centrifugation

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
2
Q

what process is used to purify proteins

A

chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
3
Q

what kind of chromatography uses the charge of protein and beads with opposite charge

A

ion exchange chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
4
Q

what is a disadvantage with ion exchange chromatography

A

not pure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
5
Q

what kind of chromatography deals with the size of proteins

A

gel filtration chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
6
Q

what is a disadvantage with gel filtration chromatography

A

not pure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
7
Q

what kind of chromatography is based off of affinity

A

affinity chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
8
Q

what is the advantage with affinity chromatography

A

pure

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
9
Q

what kind of chromatography increases purity with each chromatography step

A

serial chromatography

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
10
Q
  • short chain of aa
  • in front of target
  • use tag to purify target
A

epitope tag

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
11
Q

what does gel electrophoresis do

A

separate and visualize proteins/nucleic acids

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
12
Q

true or false: gel electrophoresis uses electricity

A

true

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
13
Q

in a gel electrophoresis big molecules move ____ and small molecules move ____

A
  • slow

- fast

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
14
Q

what type of gel is used for proteins in a gel electrophoresis

A

SDS PAGE

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
15
Q

what type of gel is used for nucleic acids in a gel electrophoresis

A

agarose

How well did you know this?
1
Not at all
2
3
4
5
Perfectly
16
Q

run samples 2x at diff pH

A

two-dimensional gel electrophoresis

17
Q

what does the western blot/immunoblotting determine

A

ID proteins after SDS PAGE gel electrophoresis

18
Q

what does the eastern blot determine

A

ID protein post translational modifications

19
Q

what does X-ray crystallography determine

A

protein structure

20
Q

what does protein sequence analysis determine

A

structure related to function

21
Q

what measures conc

A

spectrometer

22
Q

what would you apply to proteins to make them have a negative charge

A

SDS