EXAM 4- METHODS: PROTEINS Flashcards
What process is used to separate proteins
centrifugation
what process is used to purify proteins
chromatography
what kind of chromatography uses the charge of protein and beads with opposite charge
ion exchange chromatography
what is a disadvantage with ion exchange chromatography
not pure
what kind of chromatography deals with the size of proteins
gel filtration chromatography
what is a disadvantage with gel filtration chromatography
not pure
what kind of chromatography is based off of affinity
affinity chromatography
what is the advantage with affinity chromatography
pure
what kind of chromatography increases purity with each chromatography step
serial chromatography
- short chain of aa
- in front of target
- use tag to purify target
epitope tag
what does gel electrophoresis do
separate and visualize proteins/nucleic acids
true or false: gel electrophoresis uses electricity
true
in a gel electrophoresis big molecules move ____ and small molecules move ____
- slow
- fast
what type of gel is used for proteins in a gel electrophoresis
SDS PAGE
what type of gel is used for nucleic acids in a gel electrophoresis
agarose