EXAM 4- METHODS: PROTEINS

Question 1

What process is used to separate proteins

Answer 1

centrifugation

Question 2

what process is used to purify proteins

Answer 2

chromatography

Question 3

what kind of chromatography uses the charge of protein and beads with opposite charge

Answer 3

ion exchange chromatography

Question 4

what is a disadvantage with ion exchange chromatography

Answer 4

not pure

Question 5

what kind of chromatography deals with the size of proteins

Answer 5

gel filtration chromatography

Question 6

what is a disadvantage with gel filtration chromatography

Answer 6

not pure

Question 7

what kind of chromatography is based off of affinity

Answer 7

affinity chromatography

Question 8

what is the advantage with affinity chromatography

Answer 8

pure

Question 9

what kind of chromatography increases purity with each chromatography step

Answer 9

serial chromatography

Question 10

• short chain of aa
• in front of target
• use tag to purify target

Answer 10

epitope tag

Question 11

what does gel electrophoresis do

Answer 11

separate and visualize proteins/nucleic acids

Question 12

true or false: gel electrophoresis uses electricity

Answer 12

true

Question 13

in a gel electrophoresis big molecules move ____ and small molecules move ____

Answer 13

• slow

- fast

Question 14

what type of gel is used for proteins in a gel electrophoresis

Answer 14

SDS PAGE

Question 15

what type of gel is used for nucleic acids in a gel electrophoresis

Answer 15

agarose

Question 16

run samples 2x at diff pH

Answer 16

two-dimensional gel electrophoresis

Question 17

what does the western blot/immunoblotting determine

Answer 17

ID proteins after SDS PAGE gel electrophoresis

Question 18

what does the eastern blot determine

Answer 18

ID protein post translational modifications

Question 19

what does X-ray crystallography determine

Answer 19

protein structure

Question 20

what does protein sequence analysis determine

Answer 20

structure related to function

Question 21

what measures conc

Answer 21

spectrometer

Question 22

what would you apply to proteins to make them have a negative charge

Answer 22

SDS